Protein downstream processing: desing, development and application of high and low-resolution methods / ed. by N.E.Labrou. - New York: Humana press/Springer Science+Business Media, 2014. - xv, 555 p.: ill. - (Methods in molecular biology; vol.1129) (Springer protocols). - Bibliogr. at the end of the chapters. - Ind.: p.547-555. - ISBN 978-1-62703-976-5; ISSN 1064-3745 Шифр: (Pr 852/1129) 02  Место хранения:   02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents

Preface ......................................................... v Intributors .................................................... xi Part I Screening and Design Purification Strategies 1 Protein Purification: An Overview ............................ 3 Nikolaos E. Labrou 2 Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE ............................ 11 Ignacio Amadeo, Laura Mauro, Eduardo Orti, and Guillermina Forno 3 High-Throughput Process Development: I. Process Chromatography .............................................. 29 Anurag S. Rathore and Rahul Bhambure 4 High-Throughput Process Development: II. Membrane Chromatography .............................................. 39 Anurag S. Rathore and Sampath Muthukumar 5 Media Selection in Ion-Exchange Chromatography in a Single Microplate .................................................. 45 Charlotte Cabanne and Xavier Santarelli 6 High-Throughput Screening of Dye-Ligands for Chromatography .............................................. 53 Sunil Kumar and Narayan S. Punekar 7 Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips ......... 67 Sunil Chhatre and Daniel G. Bracewell Part II Low-Resolution Protein Purification Methods 8 Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline .................. 77 Zhengrong Gu 9 Aqueous Two-Phase System Strategies for the Recovery of Proteins from Plants ..................................... 89 Oscar Aguilar and Marco Rito-Palomares 10 Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins .................................. 101 Anurag S. Rathore and Rahul Bhambure 11 Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro (Affinity Ligand)- Facilitated Three Phase Partitioning (MLFTPP) .............. 111 Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta 12 Co-expression and Co-purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm ............. 125 Ario de Marco 13 Immunoglobulin Purification by Caprylic Acid ............... 137 Victor Morais, Patricia Berasain, and Hugo Massaldi Part III. High-Resolution Protein Purification Methods 14 Affinity Tags in Protein Purification and Peptide Enrichment: An Overview ........................ 147 Ana Sofia Pina, Íris L. Batalha, and Ana Cecília A. Roque 15 Expression and Purification of Large Active GST Fusion Enzymes .................................................... 169 Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa 16 Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins ......... 181 Sharon Williams, Phil Morton, and Dev Baines 17 Zbasic: A Purification Tag for Selective Ion-Exchange Recovery ................................................... 197 My Hedhammar, Johan Nilvebrant, and Sophia Hober 18 An Orthogonal Fusion Tag for Efficient Protein Purification ............................................... 205 Johan Nilvebrant, Mikael Åstrand, and Sophia Hober 19 Phage Display of Engineered Binding Proteins ............... 211 Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servé W.M. Kengen, and John van der Oost 20 Biomimetic Affinity Ligands for Protein Purification ....... 231 Isabel T. Sousa and M. Ângela Taipa 21 Synthesis and Application of Dye-Ligand Affinity Adsorbents ................................................. 263 Evangelia Chronopoulou and Nikolaos E. Labrou 22 Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification ........................ 277 Silvia Andrea Camperi, María Camila Martínez-Ceron, Silvana Laura Giudicessi, Mariela Mirta Marani, Fernando Albericio, and Osvaldo Cascone 23 Affinity Chromatography of Proteins on Monolithic Columns .. 303 E.G. Vlakh, G.A. Platonova, and T.B. Tennikova 24 Sample Displacement Batch Chromatography of Proteins ....... 325 Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter Part IV Assessing Protein Structural Integrity, Purity, and Stabilization 25 Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches ........................................ 341 Catalin E. Doneanu and Weibin Chen 26 Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications ................................ 351 Jens Sproß and Andrea Sinz 27 Proteomic Analysis of Complex Protein Samples by MALDI-TOF Mass Spectrometry. ............................ 365 Cosima Damiana Calvano, Cristina De Ceglie, and Carlo G. Zambonin 28 Modern Bioanalysis of Proteins by Electrophoretic Techniques ................................................. 381 Sona Krizkova, Marketa Ryvolova, Michal Masarik, Ondrej Zitka, Vojtech Adam, Jaromir Hubalek, Tomas Eckschlager, and Rene Kizek 29 Protein Structure Validation and Analysis with X-Ray Crystallography ............................................ 397 Anastassios C. Papageorgiou and Jesse Mattsson 30 Measuring Binding Constants of His-Tagged Proteins Using Affinity Chromatography and Ni-NTA-Immobilized Enzymes .................................................... 423 Annette C. Moser, Benjamin White, and Frank A. Kovacs 31 Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview ....................... 435 Ken-ichi Izutsu 32 Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin ...................... 443 Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda Dráberová Part V Applications/Case Studies 33 G-Protein-Coupled Receptor Expression and Purification ..... 461 Karolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos 34 (Hyper)thermophilic Enzymes: Production and Purification ... 487 Pierpaolo Falcicchio, Mark Levisson, Servé W.M. Kengen, Sotirios Koutsopoulos, and John van der Oost 35 Affinity Chromatography for Antibody Purification .......... 497 Sushrut Arora, B. Vijayalakshmi Ayyar, and Richard O'Kennedy 36 Screening and Purification of Recombinant Lignocellulolytic Enzymes .................................. 517 Evangelos Topakas and Paul Christakopoulos 37 Purification of PEGylated Proteins, with the Example of PEGylated Lysozyme and PEGylated scFv ................... 527 Anna Moosmann, Egbert Müller, and Heiner Böttinger 38 One-Step Purification of Glutamate Decarboxylase from E. coli Using Aqueous Two-Phase System ..................... 539 Wanying Yao, Jun Zhu, and Bo Sun Index ......................................................... 547