Preface ....................................................... xi
Acknowledgments ............................................. xiii
On the CD ..................................................... xv
1 Image Processing Basics ...................................... 1
1.1 Introduction ............................................. 1
1.2 Definition of Image Processing ........................... 2
1.3 Introduction to ImageJ .................................. 10
1.4 Grayscale Image Processing Basics ....................... 13
1.5 Spatial Image Processing Basics ......................... 30
1.6 The Five Classes of Image Processing .................... 32
1.7 Good Practice Considerations ............................ 34
1.8 Chapter Summary ......................................... 35
1.9 Feedback on the Self-Assessment Questions ............... 35
Reference ................................................... 38
2 Segmentation and Classification ............................. 39
2.1 Introduction ............................................ 39
2.2 Segmentation ............................................ 40
2.3 Classification .......................................... 49
2.4 Good Practice Considerations ............................ 57
2.5 Chapter Summary ......................................... 57
2.6 Feedback on the Self-Assessment Questions ............... 57
References .................................................. 59
3 Spatial Domain Filtering .................................... 61
3.1 Introduction ............................................ 61
3.2 Spatial Filtering Operations ............................ 62
3.3 Adaptive Filtering ...................................... 74
3.4 Good Practice Considerations ............................ 74
3.5 Chapter Summary ......................................... 75
3.6 Feedback on the Self-Assessment Questions ............... 75
References .................................................. 76
4 Frequency Domain Filtering .................................. 77
4.1 Introduction ............................................ 77
4.2 The Spatial Domain and the Frequency Domain ............. 78
4.3 Frequency Domain Filtering .............................. 80
4.4 Good Practice Considerations ............................ 95
4.5 Chapter Summary ......................................... 96
4.6 Feedback on the Self-Assessment Questions ............... 96
Reference .................................................. 100
5 Image Analysis Operations .................................. 101
5.1 Introduction ........................................... 101
5.2 Image Arithmetic ....................................... 102
5.3 Binary Image Operations ................................ 112
5.5 Good Practice Considerations ........................... 118
5.6 Chapter Summary ........................................ 118
5.7 Feedback on Self-Assessment Questions .................. 119
6 Image Data Formats and Image Compression ................... 121
6.1 Introduction ........................................... 121
6.2 Image Data Formats ..................................... 122
6.3 Image Compression ...................................... 130
6.4 Good Practice Considerations ........................... 137
6.5 Chapter Summary ........................................ 137
6.6 Feedback on Self-Assessment Questions .................. 138
References ................................................. 139
7 Image Restoration .......................................... 141
7.1 Introduction ........................................... 141
7.2 Blurring Arising from the Imaging System Itself ........ 142
7.3 Geometrical Distortion ................................. 148
7.4 Gray-Level Inhomogeneity ............................... 151
7.5 Good Practice Considerations ........................... 156
7.6 Chapter Summary ........................................ 156
7.7 Feedback on Self-Assessment Questions .................. 156
References ................................................. 159
8 Image Registration ......................................... 161
8.1 Introduction ........................................... 161
8.2 Image Registration ..................................... 162
8.3 Dimensionality and Number of Modalities ................ 176
8.4 Visualization of Registered Images ..................... 177
8.5 Applications ........................................... 179
8.6 Good Practice Considerations ........................... 183
8.7 Chapter Summary ........................................ 183
8.8 Feedback on Self-Assessment Questions .................. 183
References ................................................. 185
9 Visualization and 3-D Methods .............................. 187
9.1 Introduction ........................................... 187
9.2 Taxonomy for Visualization ............................. 188
9.3 Scene-Based Visualization .............................. 188
9.4 Object-Based Visualization ............................. 200
9.5 Other Visualization Methods ............................ 201
9.6 Good Practice Considerations ........................... 202
9.7 Chapter Summary ........................................ 202
9.8 Feedback on Self-Assessment Questions .................. 204
References ................................................. 206
10 Good Practice .............................................. 207
10.1 Introduction .......................................... 207
10.2 Scientific Rigor ...................................... 207
10.3 Ethical Practice ...................................... 210
10.4 Human Factors ......................................... 211
10.5 Information Technology ................................ 213
10.8 Chapter Summary ....................................... 223
10.9 Feedback on Self-Assessment Questions ................. 223
References ................................................. 224
11 Case Studies ............................................... 225
11.1 Introduction .......................................... 225
11.2 Image Enhancement ..................................... 225
11.3 Segmentation .......................................... 230
11.4 Image Compression ..................................... 241
11.5 Image Registration .................................... 250
11.6 Visualization ......................................... 256
11.7 Chapter Summary ....................................... 264
References ................................................. 264
12 For Instructors ............................................ 267
12.1 Introduction .......................................... 267
12.2 Writing Macros and Plugins for ImageJ ................. 267
12.3 Article-Based Case Studies ............................ 269
12.4 Extensions to Material in Preceding Chapters .......... 270
12.5 Publicly Available Data ............................... 278
12.6 Chapter Summary ....................................... 278
References ................................................. 279
Index ......................................................... 281
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