P-type ATPases: methods and protocols / ed. by M.Bublitz. - New York: Humana Press: Springer Science+Business Media LCC New York, 2016. - xiv, 544 p.: ill. - (Methods in molecular biology; 1377) (Springer protocols). - Bibliogr. at the end of the chapters. - Ind.: p.541-544. - ISBN 978-1-4939-3178-1; ISSN 1064-3745 Шифр: (Pr 852/1377) 02  Место хранения:   02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi 1 An Introduction to P-type ATPase Research .................... 1 Poul Nissen Part I. Protein Production, Isolation, Purification, and Stabilization 2 Purification of Na, K-ATPase from Pig Kidney ................. 5 Natalya U. Fedosova 3 Preparation of Ca2+-ATPasela Enzyme from Rabbit Sarcoplasmic Reticulum ...................................... 11 Jesper V. Møller and Claus Olesen 4 Isolation of H+, K+-ATPase-enriched Membrane Fraction from Pig Stomachs ........................................... 19 Kazuhiro Abe and Claus Olesen 5 Overproduction of PIB-Type ATPases .......................... 29 Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon 6 Coordinated Overexpression in Yeast of a P4-ATPase and Its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex ......................... 37 Hassina Azouaoui, Cédric Montigny, Aurore Jacquot, Raphaëlle Barry, Philippe Champeil, and Guillaume Lenoir 7 The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein ..................................... 57 Verena Niggli and Ernesto Carafoli 8 Expression of Na, K-ATPase and H, K-ATPase Isoforms with the Baculovirus Expression System ........................... 71 Jan B. Koenderink and Herman G.P. Swarts 9 Time-Dependent Protein Thermostability Assay ................ 79 Ilse Vandecaetsbeek and Peter Vangheluwe Part II. Activity Assays 10 Colorimetric Assays of Na, K-ATPase ......................... 89 Kathleen J. Sweadner 11 ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay ................................... 105 Pankaj Sehgal, Claus Olesen, and Jesper V. Møller 12 Antimony-Phosphomolybdate ATPase Assay ..................... 111 Gianluca Bartolommei and Francesco Tadini-Buoninsegni 13 ATPase Activity Measurements Using Radiolabeled ATP ........ 121 Herman G.P. Swarts and Jan B. Koenderink 14 Assaying P-Type ATPases Reconstituted in Liposomes ......... 127 Hans-Jürgen Apell and Bojana Damnjanovic 15 Coupling Ratio for Ca2+ Transport by Calcium Oxalate Precipitation .............................................. 157 Pankaj Sehgal, Claus Olesen, and Jesper V. Møller 16 Calcium Uptake in Crude Tissue Preparation ................. 161 Philip A. Bidwell and Evangelia G. Kranias 17 Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems ......................... 171 Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Günther-Pomorski, and Bo Højen Justesen 18 Assay of Flippase Activity in Proteoliposomes Using Fluorescent Lipid Derivatives .............................. 181 Magdalena Marek and Thomas Günther-Pomorski Part III. In Vitro Functional Studies 19 The Use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases .................................................... 195 Stefania J. Danko and Hiroshi Suzuki 20 Phosphorylation/Dephosphorylation Assays ................... 211 Hiroshi Suzuki 21 Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function ................................................... 227 Pankaj Sehgal, Claus Olesen, and Jesper V. Møller Part IV. Ligand Binding Studies 22 Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [γ-32P]TNP-8N3-ATP Photolabeling ........................... 233 Johannes D. Clausen, David B. Mcintosh, David G. Woolley, and Jens Peter Andersen 23 Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+ ................................................. 261 Pankaj Sehgal, Claus Olesen, and Jesper V. Møller 24 Assay of Copper Transfer and Binding to PIB-ATPases ........ 267 Teresita Padilla-Benavides and José M. Argüello Part V. Electrophysiology 25 Voltage Clamp Fluorometry of P-Type ATPases ................ 281 Robert E. Dempski 26 Electrophysiological Measurements on Solid Supported Membranes .................................................. 293 Francesco Tadini-Buoninsegni and Gianluca Bartolommei 27 Electrophysiological Characterization of Na, K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping ........................................... 305 Florian Hilbers and Hanne Poulsen Part VI. Functional Studies by Cell Culture and Transgenic Animals 28 Functional Studies of Na+,K+-ATPase Using Transfected Cell Cultures .............................................. 321 Elena Arystarkhova and Kathleen J. Sweadner 29 HPLC Neurotransmitter Analysis ............................. 333 Thomas Hellesøe Holm, Toke Jost Isaksen, and Karin Lykke-Hartmann 30 Behavior Test Relevant to α2/α3Na+/K+-ATPase Gene Modified Mouse Models ...................................... 341 Toke Jost Isaksen, Thomas Hellesøe Holm, and Karin Lykke- Hartmann 31 Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning .............................................. 353 Canan Doganli, Jens Randel Nyengaard, and Karin Lykke- Hartmann 32 Whole-Mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 Days Post Fertilization (dpf)) ...... 365 Canan Doganli, Lucas Bukata, and Karin Lykke-Hartmann 33 Cell-Based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives .......................................... 371 Maria S. Jensen, Sara Costa, Thomas Günther-Pomorski, and Rosa L. López-Marqués 34 Transient Expression of P-type ATPases in Tobacco Epidermal Cells ............................................ 383 Lisbeth R. Poulsen, Michael G. Palmgren, and Rosa L. López-Marqués Part VII. Lipid Techniques 35 Lipid Exchange by Ultracentrifugation ...................... 397 Nikoіaj During Drachmann and Claus Olesen 36 Reconstitution of Na+, K+-ATPase in Nanodiscs .............. 403 Jonas Lindholt Gregersen, Natalya U. Fedosova, Poul Nissen, and Thomas Boesen Part VIII. Crystallization 37 Crystallization of P-type ATPases by the High Lipid- Detergent (HiLiDe) Method .................................. 413 Oleg Sitsel, Kaituo Wang, Xiangyu Liu, and Pontus Gourdon 38 Two-Dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography ............................... 421 John Paul Glaves, Joseph O. Primeau, and Howard S. Young 39 Two-Dimensional Crystallization of Gastric H+, K*-ATPase for Structural Analysis by Electron Crystallography ........ 443 Kazuhiro Abe Part IX. Computational Approaches in Analyzing P-Type ATPases 40 MD Simulations of P-Type ATPases in a Lipid Bilayer System ..................................................... 459 Henriette Elisabeth Autzen and Maria Musgaard 41 Computational Classification of P-Type ATPases ............. 493 Dan Sondergaard, Michael Knudsen, and Christian Norgaard Storm Pedersen 42 Molecular Modeling of Fluorescent SERCA Biosensors ......... 503 Bengt Svensson, Joseph M. Autry, and David D. Thomas 43 How to Compare, Analyze, and Morph Between Crystal Structures of Different Conformations: The P-Type ATPase Example .................................................... 523 Jesper L. Karlsen and Maike Bublitz Index ......................................................... 541