Preface ......................................................... v
Contributors ................................................... ix
1 A High-Throughput Yeast Two-Hybrid Protocol to Determine
Virus-Host Protein Interactions .............................. 1
Hannah Striebinger, Manfred Koegl, and Susanne M. Bailer
2 Analysis of Protein-Protein Interactions Using LUMIER
Assays ...................................................... 17
Sonja Blasche and Manfred Koegl
3 Detection of Protein Interactions During Virus Infection
by Bimolecular Fluorescence Complementation ................. 29
Stefan Becker and Jens von Einem
4 Discovery of Host-Viral Protein Complexes During Infection .. 43
Daniell L. Rowles, Scott S. Terbune, and Ileana M. Cristea
5 Screening for Host Proteins with Pro- and Antiviral
Activity Using High-Throughput RNAi ......................... 71
Samantha J. Griffiths
6 High Resolution Gene Expression Profiling of RNA
Synthesis, Processing, and Decay by Metabolic Labeling of
Newly Transcribed RNA Using 4-Thiouridine ................... 91
Lars Dölken
7 Isolation and Characterization of Pathogen-Bearing
Endosomes Enable Analysis of Endosomal Escape and
Identification of New Cellular Cofactors of Infection ...... 101
Konstanze D. Scheffer, Ruth Popa-Wagner, and Luise Florin
8 Computational Analysis of Virus-Host Interactomes .......... 115
Caroline C. Friedel
9 Interspecies Heterokaryon Assay to Characterize the
Nucleocytoplasmic Shuttling of Herpesviral Proteins ........ 131
Shuai Wang, Kezheng Wang, and Chunfu Zheng
10 Detection of Integrated Herpesvirus Genomes by
Fluorescence In Situ Hybridization (FISH) .................. 141
Benedikt B. Kaufer
11 Fast Generation of Stable Cell Lines Expressing
Fluorescent Marker Molecules to Study Pathogen Induced
Processes .................................................. 153
Jens Bernhard Bosse, Jessica Rogues, and Harald Wodrich
12 Determination of HSV-1 Infectivity by Plaque Assay and
a Luciferase Reporter Cell Line ............................ 171
Diana Lieber and Susanne M. Bailer
13 Generation of a Stable Cell Line for Constitutive miRNA
Expression ................................................. 183
Diana Lieber
14 Applications for a Dual Fluorescent Human Cytomegalovirus
in the Analysis of Viral Entry ............................. 201
Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger
15 High Temporal Resolution Imaging Reveals Endosomal
Membrane Penetration and Escape of Adenoviruses in Real
Time ....................................................... 211
Ruben Martinez, Andrew M. Burrage, Christopher
M. Wiethoff, and Harald Wodrich
16 Three-Dimensional Visualization of Virus-Infected Cells
by Serial Sectioning: An Electron Microscopic Study Using
Resin Embedded Cells ....................................... 227
Martin Schauflinger, Clarissa Villinger, and Paul
Walther
17 3D-Tissue Model for Herpes Simplex Virus-1 Infections ...... 239
Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer
18 In Vivo Visualization of Encephalitic Lesions in Herpes
Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic
Resonance Imaging (MRI) .................................... 253
Wali Hafezi and Verena Hoerr
19 Detection of Antigen-Specific T Cells Based on
Intracellular Cytokine Staining Using Flow-Cytometry ....... 267
Tina Schmidt and Martina Sester
20 Functional NK Cell Cytotoxicity Assays Against Virus
Infected Cells ............................................. 275
Rebecca J. Aicheler and Richard J. Stanton
21 Assessment of Natural Killer Cell Responses to Human
Cytomegalovirus-Infected Macrophages ....................... 289
Zeguang Wu, Giada Frascaroli, and Thomas Mertens
22 Analysis of Cell Migration During Human Cytomegalovirus
(HCMV) Infection ........................................... 299
Stefania Varani and Giada Frascaroli
23 Gene Targeting in Mice: A Review ........................... 315
Hicham Bouabe and Klaus Okkenhaug
24 A Protocol for Construction of Gene Targeting Vectors
and Generation of Homologous Recombinant Embryonic Stem
Cells ...................................................... 337
Hicham Bouabe and Klaus Okkenhaug
25 Measurement of Mouse Cytomegalovirus-Induced Interferon-β
with Immortalized Luciferase Reporter Cells ................ 355
Fvgenia Scheibe, Stefan Lienenklaus, Tobias May,
Vladimir Gonçalves Magalhães, Siegfried Weiss, and Melanie
M. Brinkmann
Index ......................................................... 367
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