Preface ......................................................... v
Contributors ................................................... ix
Part I. Imaging Morphogenesis
1 Probing Regional Mechanical Properties of Embryonic Tissue
Using Microindentation and Optical Coherence Tomography ...... 3
Benjamen A. Filas, Gang Xu, and Larry A. Taber
2 Hemodynamic Flow Visualization of Early Embryonic Great
Vessels Using μPIV .......................................... 17
Selda Goktas, Chia-Yuan Chen, William J. Kowalski, and
Kerem Pekkan
3 Using Correlative Light and Electron Microscopy to Study
Zebrafish Vascular Morphogenesis ............................ 31
Jacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien
Vermot
4 Micro/Nano-Computed Tomography Technology for Quantitative
Dynamic, Multi-scale Imaging of Morphogenesis ............... 47
Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan
T. Butcher
5 Imaging the Dorsal-Ventral Axis of Live and Fixed
Drosophila melanogaster Embryos ............................. 63
Sophia N. Carrell and Gregory T. Reeves
6 Light Sheet-Based Imaging and Analysis of Early
Embryogenesis in the Fruit Fly .............................. 79
Khaled Khairy, William C. Lemon, Fernando Amat, and
Philipp J. Keller
7 Quantitative Image Analysis of Cell Behavior and Molecular
Dynamics During Tissue Morphogenesis ........................ 99
Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez
8 A Multiplex Fluorescent In Situ Hybridization Protocol for
Clonal Analysis of Drosophila Oogenesis .................... 115
Lily S. Cheung and Stanislav Shvartsman
9 Active Cell and ECM Movements During Development ........... 123
Anastasiia Aleksandrova, Brenda J. Rongish, Charles
D. Little, and András Czirók
Part II. Culture Models of Morphogenesis
10 3D Culture Assays of Murine Mammary Branching Morphogenesis
and Epithelial Invasion ..................................... 135
Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner,
Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald
11 Culture of Mouse Embryonic Foregut Explants ................. 163
Felicia Chen and Wellington V. Cardoso
12 Investigating Human Vascular Tube Morphogenesis and
Maturation Using Endothelial Cell-Pericyte Co-cultures and
a Doxycycline-Inducible Genetic System in 3D Extracellular
Matrices .................................................... 171
Stephanie L.K. Bowers, Chun-Xia Meng, Matthew T. Davis,
and George E. Davis
13 Three-Dimensional Traction Force Microscopy of Engineered
Epithelial Tissues .......................................... 191
Alexandra S. Piotrowski, Victor D. Varner, Nikolce
Gjorevski, and Celeste M. Nelson
Part III. Manipulating Cells and Tissues In Vivo
14 Probing Cell Mechanics with Subcellular Laser Dissection
of Actomyosin Networks in the Early Developing Drosophila
Embryo ..................................................... 209
M. Rauzi and P.-F. Lenne
15 UV Laser Ablation to Measure Cell and Tissue-Generated
Forces in the Zebrafish Embryo In Vivo and Ex Vivo ......... 219
Michael Smutny, Martin Behrndt, Pedro Campinho, Verena
Ruprecht, and Carl-Philipp Heisenberg
16 Measurement of Intercellular Cohesion by Tissue Surface
Tensiometry ................................................ 237
Ramsey A. Foty
17 Quail-Chick Chimeras and Eye Development ................... 255
Sinu Jasrapuria Agrawal and Peter Y. Lwigale
Part IV. Emerging Models of Tissue Morphogenesis
18 Studying Epithelial Morphogenesis in Dictyostelium ......... 267
Daniel J. Dickinson, W. James Nelson, and William I. Weis
19 Primary Cell Cultures of Regenerating Holothurian Tissues .. 283
Samir A. Bello, Ricardo J. Abreu-Irizarry,
and José E. García-Arrarás
Part V. Computational Models of Tissue Morphogenesis
20 Large-Scale Parameter Studies of Cell-Based Models of
Tissue Morphogenesis Using CompuCell3D or Virtual Leaf ..... 301
Margriet M. Palm and Roeland M.H. Merks
21 Simulating Tissue Morphogenesis and Signaling .............. 323
Dagmar Iber, Simon Tanaka, Patrick Fried, Philipp Germann,
and Denis Menshykau
22 Elasticity-Based Targeted Growth Models of Morphogenesis ... 339
Patrick W. Alford
Index ......................................................... 35i
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