Preface ......................................................... V
Contributors ................................................ ix
1 Generating Primary Cultures of Murine Cardiac Myocytes
and Cardiac Fibroblasts to Study Viral Myocarditis ........... 1
Barbara Sherry
2 Enrichment of Cardiomyocytes in Primary Cultures of Murine
Neonatal Hearts ............................................. 17
Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna
Vidyasekar, and Rama S. Verma
3 Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes
in Clinical and Experimental Cardiomyopathy ................. 27
Scot J. Matkovich and Gerald W. Dorn II
4 Next-Generation Sequencing Technology in the Genetics
of Cardiovascular Disease ................................... 51
Cecilia Vecoli
5 Computational Cardiac Electrophysiology: Implementing
Mathematical Models of Cardiomyocytes to Simulate Action
Potentials of the Heart ..................................... 65
Michael M. Bell and Elizabeth M. Cherry
6 Methods of Myofibrillogenesis Modeling ...................... 75
Nancy K. Drew and Anna Grosberg
7 Using the Mechanical Bidomain Model to Analyze the
Biomechanical Behavior of Cardiomyocytes .................... 93
Bradley J. Roth
8 Fabrication of a Myocardial Patch with Cells Differentiated
from Human-Induced Pluripotent Stem Cells .................. 103
Lei Ye, Joydeep Basu, and Jianyi Zhang
9 Efficient Differentiation of Cardiomyocytes from Human
Pluripotent Stem Cells with Growth Factors ................. 115
Rajneesh Jha, Ren-He Xu, and Chunhui Xu
10 Isolation, Culturing, and Characterization of Cardiac
Muscle Cells from Nonhuman Primate Heart Tissue ............ 133
Steven M. Hoynowski and John W. Ludlow
11 Mouse Embryonic Stem Cell-Derived Cardiac Myocytes
in a Cell Culture Dish ..................................... 145
Carley Glass, Reetu Singla, Anshu Arora, and Dinender
K. Singla
12 Cryopreservation of Neonatal Cardiomyocytes ................ 153
Adam C. Vandergriff, M. Taylor Hensley, and Ke Cheng
13 Evaluation of Sarcomeric Organization in Human
Pluripotent Stem Cell-Derived Cardiomyocytes ............... 161
Chrishan J.A. Ramachandra and Winston Shim
14 Electro tonic Coupled Metabolic Purification of Chick
Cardiomyocytes ............................................. 167
Winston Shim, Haiyang Yu, K.P. Myu Mai Ja, Muhammad
Parasuram, Kee Pah Lim, and Philip Wong
15 Gene Transfer into Cardiac Myocytes ........................ 177
Sarah E. Lang and Margaret V. Westfall
16 Analysis of 4D Myocardial Wall Motion During Early Stages
of Chick Heart Development ................................. 191
Madeline Midgett and Sandra Rugonyi
Index ......................................................... 213
|
