1 Membrane Protein Production for Structural Analysis .......... 1
Isabelle Mus-Veteau, Pascal Demange and Francesca Zito
2 Membrane Protein Quality Control in Cell-Free Expression
Systems: Tools, Strategies and Case Studies ................. 45
Davide Proverbio, Erik Henrich, Erika Orbán, Volker
Dötsch and Frank Bernhard
3 Bacterial Expression and Stabilization of GPCRs ............. 71
Jean-Louis Banères
4 Membrane Protein Production in Escherichia coli: Overview
and Protocols ............................................... 87
Georges Hattab, Annabelle Y.T. Suisse, Oana Ilioaia,
Marina Casiraghi, Manuela Dezi, Xavier L. Warnet, Dror
E. Warschawski, Karine Moncoq, Manuela Zoonens and Bruno
Miroux
5 Lactococcus lactis: Recent Developments in Functional
Expression of Membrane Proteins ............................ 107
Sana Bakari, François André, Daphné Seigneurin-Berny,
Marcel Delaforge, Norbert Rolland and Annie Frelet-
Barrand
6 Overexpression of Membrane Proteins in Saccharomyces
cerevisiae for Structural and Functional Studies: A Focus
on the Rabbit Ca2+-ATPase Sercala and on the Yeast Lipid
"Flippase" Complex Drs2p/Cdc50p ............................ 133
Cédric Montigny, Hassina Azouaoui, Aurore Jacquot, Marc
le Maire, Christine Jaxel, Philippe Champeil and
Guillaume Lenoir
7 Amphipols: A General Introduction and Some Protocols ....... 173
Manuela Zoonens, Francesca Zito, Karen L. Martinez and
Jean-Luc Popot
8 New Amphiphiies to Handle Membrane Proteins: "Ménage à
Trois" Between Chemistry, Physical Chemistry, and
Biochemistry ............................................... 205
Grégory Durand, Maher Abla, Christine Ebel and Cécile
Breyton
9 Building Model Membranes with Lipids and Proteins: Dangers
and Challenges ............................................. 253
James N. Sturgis
10 Analytical Ultracentrifugation and Size-Exclusion
Chromatography Coupled with Light Scattering for the
Characterization of Membrane Proteins in Solution .......... 267
Aline Le Roy, Cécile Breyton and Christine Ebel
11 Lipidic Cubic Phase Technologies for Structural Studies
of Membrane Proteins ....................................... 289
Andrii Ishchenko, Enrique Abola and Vadim Cherezov
12 Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or
Intact Cells: The Hitchhiker's Guide to the Study of
Membrane Proteins by NMR ................................... 315
Laurent J. Catoire, Xavier L. Warnet and Dror E.
Warschawski
13 Foundations of Biomolecular Simulations: A Critical
Introduction to Homology Modeling, Molecular Dynamics
Simulations, and Free Energy Calculations of Membrane
Proteins ................................................... 347
Marc Baaden, Jérôme Hénin and Antoine Taly
14 Structural Studies of TSPO, a Mitochondrial Membrane
Protein .................................................... 393
Jean-Jacques Lacapere, Soria Iatmanen-Harbi, Lucile
Senicourt, Olivier Lequin, Piotr Tekely, Rudra N.
Purasottam, Petra Hellwig, Sebastien Kriegel, Stephanie
Ravaud, Celine Juillan-Binard, Eva Pebay Peyroula and
Vassilios Papadopoulos
Index ......................................................... 423
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