Dedication ...................................................... v
Preface ....................................................... vii
Contributors ................................................... xi
Part I. Fundamental Aspects and Freezing Technology
1 Principles of Cryopreservation ............................... 3
David E. Pegg
2 Principles of Cryopreservation by Vitrification ............. 21
Gregory M. Fahy and Brian Wowk
3 Modeling and Optimization of Cryopreservation ............... 83
James D. Benson
4 The Principles of Freeze-Drying ............................ 121
Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward
Part II. Methods to Study Freezing and Drying Processes
5 Use of In Situ Fourier Transform Infrared Spectroscopy
to Study Freezing and Drying of Cells ...................... 147
Willem F. Wolkers and Harriette Oldenhof
6 Calorimetric Analysis of Cryopreservation and Freeze-
Drying Formulations ........................................ 163
Wendell Q. Sun
7 Measurement of Intracellular Ice Formation Kinetics by
High-Speed Video Cryomicroscopy ............................ 181
Jens O.M. Karlsson
8 Laser Scanning Microscopy in Cryobiology ................... 229
Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann
9 Low-Temperature Electron Microscopy: Techniques and
Protocols .................................................. 243
Roland A. Fleck
Part III Cryopreservation Protocols
10 Cryopreservation of Semen from Domestic Livestock .......... 277
Harald Sieme and Harriëtte Oldenhof
11 Cryopreservation of Mammalian Oocytes ...................... 289
Victoria Keros and Barry J. Fuller
12 Vitrification: A Simple and Successful Method for
Cryostorage of Human Blastocysts ........................... 305
Juergen Liebermann
13 Efficient Cryopreservation of Human Pluripotent Stem Cells
by Surface-Based Vitrification ............................. 321
Julia C. Neubauer, Axel F. Beier, Niels Geijsen, and
Heiko Zimmermann
14 Cryopreservation of Greenshell™ Mussel (Perna canaliculus)
Sperm ...................................................... 329
Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay
T. McGowan, and H. Robin Tervit
15 Membrane Modification Strategies for Cryopreservation ...... 337
Phillip H. Purdy and James K. Graham
16 Sperm Cleanup and Centrifugation Processing for
Cryopreservation ........................................... 343
Harald Sieme and Harriette Oldenhof
17 Cryopreservation of Red Blood Cells ........................ 353
Johan W. Lagerberg
18 Cord Blood Clinical Processing, Cryopreservation, and
Storage .................................................... 369
Heidi Elmoazzen and Jelena L. Holovati
19 Directional Freezing for Large Volume Cryopreservation ..... 381
Joseph Saragusty
20 Vitrification of Heart Valve Tissues ....................... 399
Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene,
and Lia H. Campbell
21 Cryopreservation of Plant Cell Lines ....................... 423
Heinz Martin Schumacher, Martina Westphal, and Elke
Heine-Dobbernack
22 Writing Standard Operating Procedures (SOPs) for
Cryostorage Protocols: Using Shoot Meristem
Cryopreservation as an Example ............................. 431
Keith Harding and Erica E. Benson
Part IV Freeze-Drying Protocols
23 Freeze-Drying of Proteins .................................. 459
Baolin Liu and Xinli Zhou
24 Freeze-Drying of Lactic Acid Bacteria ...................... 477
Fernanda Fonseca, Stéphanie Cenard, and Stéphanie Passot
25 Freeze-Drying of Mammalian Sperm ........................... 489
Levent Keskintepe and AH Eroglu
26 Freeze-Drying of Decellularized Heart Valve Tissues ........ 499
Willem F. Wolkers and Andres Hilfiker
Index ......................................................... 507
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