1 Introduction ................................................. 1
Norma M. Allewell, Linda О. Narhi, and Ivan Rayment
2 Structural, Physical, and Chemical Principles ............... 17
Norma M. Allewell, Linda О. Narhi, and Ivan Rayment
Part I The Experimental Tools of Molecular Biophysics
3 Optical Spectroscopic Methods for the Analysis
of Biological Macromolecules ................................ 33
Linda O. Narhi, Cynthia H. Li, Ranjini Ramachander, Juraj
Svitel, and Yijia Jiang
4 Diffraction and Scattering by X-Rays and Neutrons ........... 91
Ivan Rayment
5 Nuclear Magnetic Resonance Spectroscopy .................... 113
Thomas C. Pochapsky and Susan Sondej Pochapsky
6 Electron Paramagnetic Resonance Spectroscopy ............... 175
John H. Golbeck and Art van der Est
7 Mass Spectrometry .......................................... 215
Igor A. Kaltashov and Cedric E. Bobst
8 Single-Molecule Methods .................................... 257
Paul J. Bujalowski, Michael Sherman, and Andres
F. Oberhauser
Part II Biological Macromolecules as Molecular Machines:
Three Examples
9 Helicase Unwinding at the Replication Fork ................. 291
Divya Nandakumar and Smita S. Patel
10 Rotary Motor ATPases ....................................... 313
Stephan Wilkens
11 Biophysical Approaches to Understanding the Action
of Myosin as a Molecular Machine ........................... 341
Mihály Kovács and Andrвs Málnási-Csizmadia
Part III Future Prospects
12 Future Prospects ........................................... 365
Norma M. Allewell, Igor A. Kaltashov, Linda O. Narhi, and
Ivan Rayment
Index ......................................................... 381
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