Series Preface ................................................. ix
Foreword: Britton Chance - Appreciation ........................ xi
Preface ...................................................... xvii
Editors ....................................................... xix
Contributors .................................................. xxi
Abbreviations ............................................... xxiii
SECTION I Biochemical, Biological, and Biophysical Background
1 Mitochondria and Energy Metabolism: Networks, Mechanisms,
and Control .................................................. 3
Ilmo E. Hassinen
2 Intracellular Autofluorescent Species: Structure,
Spectroscopy, and Photophysics .............................. 41
Nobuhiro Ohta and Takakazu Nakabayashi
SECTION II Autofluorescence Imaging Techniques: Fundamentals
and Applications
3 One-Photon Autofluorescence Microscopy ...................... 67
Narasimhan Rajaram and Nirmala Ramanujam
4 Autofluorescence Lifetime Imaging ........................... 77
Michael G. Nichols, Kristina Ward, Lyandysha V. Zholudeva,
Heather Jensen Smith, and Richard Hallworth
5 Polarization Imaging of Cellular Autofluorescence .......... 107
Harshad D. Vishwasrao, Qianru Yu, Kuravi Hewawasam, and
Ahmed A. Heikal
6 Real-Time In Vivo Monitoring of Cellular Energy
Metabolism ................................................. 137
Avraham Mayevsky and Efrat Barbiro-Michaely
7 Tryptophan as an Alternative Biomarker for Cellular
Energy Metabolism .......................................... 157
Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy
8 Alternative Approaches to Optical Sensing of the Redox
State ...................................................... 179
Yi Yang
SECTION III Natural Biomarkers for Biochemical and Biological
Studies
9 Spatiotemporal Detection of NADH-Linked Enzyme Activities
in Single Cell Metabolism .................................. 201
V. Krishnan Ramanujan
10 NAD(P)H and FAD as Biomarkers for Programmed Cell Death .... 215
Hsing-Wen Wang
11 Monitoring Stem Cell Differentiation in Engineered
Tissues .................................................... 225
Kyle P. Quinn and Irene Georgakoudi
SECTION IV Autofluorescence as a Diagnostic Tool in Medicine
and Health
12 Autofluorescence-Assisted Examination of Cardiovascular
System Physiology and Pathology ............................ 245
Atebeta Marcek Chorvatova
13 Autofluorescence Perspective of Cancer Diagnostics ......... 273
Lin Z. Li and Nannan Sun
14 Dynamic Imaging of Intracellular Coenzyme Autofluorescence
in Intact Pancreatic Islets ................................ 299
Alan K. Lam and Jonathan V. Rocheleau
15 Autofluorescence Diagnostics of Ophthalmic Diseases ........ 317
Dietrich Schweitzer
16 Pathogen Effects on Energy Metabolism in Host Cells ........ 347
Márta Szaszák and Jan Rupp
Index ......................................................... 373
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