Series Preface ................................................ vii
Foreword ....................................................... ix
Preface ...................................................... xiii
Editors ........................................................ xv
Contributors ................................................. xvii
SECTION I Principles of SHG Imaging
1 Theoretical Framework for SHG Microscopy: A Matrix-Based
Approach ..................................................... 3
Christopher M. Dettmar and Garth J. Simpson
2 How to Build an SHG Apparatus ............................... 21
Riccardo Cicchi, Leonardo Sacconi, Francesco Vanzi, and
Francesco S. Pavone
3 THG Microscopy of Cells and Tissues: Contrast Mechanisms
and Applications ............................................ 51
Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire
4 SHG Microscopy and Its Comparison with THG, CARS, and
Multiphoton Excited Fluorescence Imaging .................... 81
Xiyi Chen and Paul J. Campagnola
SECTION II Biomedical Imaging Using SHG
5 Molecular Structure and Order with Second-Harmonic
Generation Microscopy ...................................... 103
Francesco Vanzi, Leonardo Sacconi, Riccardo Cicchi,
Chiara Stringari, and Francesco S. Pavone
6 3D SHG Imaging and Analysis of Fibrillar Collagen
Organization ............................................... 125
Paul J. Campagnola
7 Second-Harmonic Generation Imaging of Microtubules ......... 151
Alex C. Kwan
8 SHG and Optical Clearing ................................... 169
Oleg Nadiarnykh and Paul J. Campagnola
9 Holographic SHG Imaging .................................... 191
Etienne Shaffer and Christian Depeursinge
10 Imaging Membrane Potential with SHG ........................ 229
Mutsuo Nuriya and Rafael Yuste
SECTION III Applications of SHG
11 Second-Harmonic Generation Imaging of Self-Assembled
Collagen Gels .............................................. 245
Christopher В. Raub, Bruce J. Tromberg, and Steven
C. George
12 SHG and Multiphoton Fluorescence Imaging of the Eye ........ 271
Chiu-Mei Hsueh, Po-Sheng Hu, Wen Lo, Shean-Jen Chen,
Hsin-Yuan Tan, and Chen-Yuan Dong
13 Multiphoton Excitation Imaging of the Arterial Vascular
Bed ........................................................ 289
Edward B. Neufeld, Bertrand M. Lucotte, and Robert
S. Balaban
14 Combined SHG/THG Imaging ................................... 309
Szu-Yu Chen and Chi-Kuang Sun
15 SHG Imaging of Collagen and Application to Fibrosis
Quantization ............................................... 349
Marie-Claire Schanne-Klein
16 Quantitative Approaches for Studying the Role of Collagen
in Breast Cancer Invasion and Progression .................. 373
Caroline A. Schneider, Carolyn A. Pehlke, Karissa
Tilbury, Ruth Sullivan, Kevin W. Eliceiri, and Patricia
J. Keely
17 SHG in Tumors: Scattering and Polarization ................. 391
Seth W. Perry, Xiaoxing Han, and Edward B. Brown
18 SHG Imaging for Tissue Engineering Applications ............ 409
Annika Enejder and Christian Brackmann
Index ......................................................... 427
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