Preface ......................................................... v
Contributors ................................................... ix
Part I Culture of Zygotic Embryos: Fundamental Studies
1 Zygotic Embryo Culture: Ал Overview .......................... 3
Tegan M. Haslam and Edward C. Yeung
2 In vitro Fertilization with Rice Gametes: Production
of Zygotes and Zygote and Embryo Culture .................... 17
Takashi Okamoto
3 Canola Zygotic Embryo Culture ............................... 29
Nicole S. Ramesar-Fortner and Edward C. Yeung
4 Immature Seeds and Embryos of Medicago truncatula Cultured
In vitro .................................................... 39
Sergio J. Ochatt
5 In vitro Culture and Germination of Terrestrial Asian
Orchid Seeds ................................................ 53
Yung-I. Lee
6 In vitro Culture of Coconut (Cocos nucifera L.) Zygotic
Embryos ..................................................... 63
Florent Engelmann, Bernard Malaurie, and Oulo N'Nan
Part II Application of Embryo Culture Techniques: Embryo
Rescue
7 Immature Embryo Rescue and Culture .......................... 75
Xiuli Shen, Fred G. Gmitter, Jr., and Jude W. Grosser
8 Chickpea Hybridization Using In vitro Techniques ............ 93
Nalini Mallikarjuna and Fred J. Muehlbauer
9 Muskmelon Embryo Rescue Techniques using In vitro Embryo
Culture .................................................... 107
Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón,
and Neftalí Ochoa-Alejo
10 Phaseolus Immature Embryo Rescue Technology ................ 117
Pascal Geerts, André Toussaint, Guy Mergeai, and
Jean-Pierre Baudoin
11 Wide Crossing in Lentil through Embryo Rescue .............. 131
Richard Fratini and Maria L. Ruiz
12 Generation of Interspecific Hybrids of Trifolium Using
Embryo Rescue Techniques ................................... 141
Ajoy Kumar Roy, Devendra Ram Malaviya, and Pankaj Kaushal
Part III Cryopreservation of Embryos
13 Cryopreservation of Embryos: An Overview ................... 155
Florent Engelmann
14 Cryogenic Technologies for the Long-Term Storage of Citrus
Germplasm .................................................. 185
Anna De Carlo, Maurizio Lambardi, and Elif Aylin Ozudogru
15 Cryopreservation of Zygotic Embryonic Axes and Somatic
Embryos of European Chestnut ............................... 201
Ana M. Vieitez, M. Carmen San-José, and Elena Corredoira
16 Cryopreservation of Ilex Immature Zygotic Embryos .......... 215
Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia
Luna, Ana Gonzalez, and Hebe Rey
Part IV The Use of Zygotic Embryos as Explants for Somatic
Embryogenesis and/or Organogenesis
17 The Use of Zygotic Embryos as Explants for In Vitro
Propagation: An Overview ................................... 229
Mohamed Elhiti and Claudio Stasolla
18 Somatic Embryogenesis and Plant Regeneration in the
Culture of Arabidopsis thaliana (L.) Heynh. Immature
Zygotic Embryos ............................................ 257
Malgorzata D. Gaj
19 Pine Somatic Embryogenesis Using Zygotic Embryos as
Explants ................................................... 267
Gerald S. Pullman and Kylie Bucalo
20 Micropropagation of Phalaenopsis Orchids via Protocorms
and Protocorm-Like Bodies .................................. 293
Kee Yoeup Paek, Eun Joo Hahn, and So Young Park
Part V Transformation Using Zygotic Embryos as Explants
21 Genetic Transformation Protocols Using Zygotic Embryos
as Explants: An Overview ................................... 309
Muhammad Tahir, Ejaz A. Waraich, and Claudio Stasolla
22 Genetic Transformation Using Maize Immature Zygotic
Embryos .................................................... 327
Bronwyn Frame, Marcy Main, Rosemarie Schick, and Kan Wang
23 Biolistic-Mediated Transformation Protocols for Maize and
Pearl Millet Using Pre-Cultured Immature Zygotic Embryos
and Embryogenic Tissue ..................................... 343
Martha M. O'Kennedy, Hester C. Stark, and Nosisa Dube
24 Agrobacterium tumefaciens-Mediated Genetic Transformation
of Cereals Using Immature Embryos .......................... 355
Ashok K. Shrawat and Allen G. Good
Index ......................................................... 373
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