17th IBS International Scientific Committee ..................... v
17th IBS Local Organizing Committee ............................ vi
Preface ....................................................... vii
Honouring James A. Brierley - A Leading Biohydrometallurgist ... ix
IN MEMORIAM Gregorii I. Karavaiko .............................. xi
I. Honorary Lecture
Biohydrometallurgy - This Microbiologist's Perspective
J.A. Brierley ................................................ 3
II. Bioleaching Technology and Biobenefication
(edited by F. Glombitza)
Keynote Paper
Status and Challenges of the Bioleaching Technologies
D.H.R. Morin ................................................ 13
Extended Abstracts
Optimization of ROM Sulfide Leaching at Anglo American Chile
Los Bronces Division
E. Saldívar and G. Zárate ................................... 21
Development of a Biotechnological Process for Reprocessing
Flotation Tailings from Almalyk Mining and Metallurgical
Complex, Rep. of Uzbekistan
M.G. Sagdieva, S.I. Borminskiy, K.S. Sanakulov and
O.P. Vasilenok .............................................. 26
Talvivaara Black Schist Bioheapleaching Demonstration Plant
M. Riekkola-Vanhanen ........................................ 30
Comparison of the Bioleaching Effect of Mesophilic (35°C)
and Thermophilic (45°C) Bacteria on the TIZAPA Tailings
R.E. Rivera-Santillán and V. Becerril-Reyes ................. 34
Application Potential of Biohydrometallurgy in the Iranian
Mining Industry
M. Ranjbar, M. Schaffie, M. Pazouki, R. Ghazi, A. Akbary,
S. Zanddevakili, S.A. Seied Baghery and Z. Manafi ........... 38
The Bioshale Project: Search for a Sustainable Way of
Exploiting Black Shale Ores Using Biotechnology
P. D'Hugues, A. Grotowski, A. Luszczkiewicz, Z. Sadowski,
T. Farbiszewska, A. Sklodowska, K. Loukola-Ruskeeniemi,
J. Langwaldt, J. Palma, P.R. Norris, F. Glombitza,
S. Groudev, J. Pasava and D.B. Johnson ...................... 42
Removal of Organically Bound Sulfur From Oil Shale by
Iron(III)-Ion Generated-Regenerated from Pyrite by the Action
of Acidithiobacillus ferrooxidans
V.P. Beskoski, J. Milic, B. Mandic, M. Takic and
M.M. Vrvic .................................................. 46
Mobilization of Silver, Gold, and Platinum from Solid
Materials by HCN-Forming Microorganisms
H. Brandl, S. Lehmann and M.A. Faramarzi .................... 50
High-Rate Fluidized-Bed Ferric Sulfate Generation for
Hydrometallurgical Applications
J.A. Puhakka, P.H.M. Kinnunen, T. van der Meer, B. Özkaya,
E. Sahinkaya, A.H. Kaksonen and P. Nurmi .................... 54
Development of a Tank Bioleaching Process for the Treatment
of Complex Cu-Polymetallic Concentrates
M. Gericke, H.H. Muller, P.J. van Staden and A. Pinches ..... 58
Continuous Bioleaching of a Cobaltiferous Pyrite in Stirred
Reactors: Population Dynamics and EPS Production
vs. Bioleaching Performances
P. D'Hugues, C. Joulian, P. Spolaore, C. Michel,
F. Garrido and D. Morin ..................................... 62
Characterisation of Factors in the Bacterial Leaching of
Nickel Laterites Using Statistical Design of Experiments
G.S. Simate and S. Ndlovu ................................... 66
Modeling the Auto-Thermal Performance of a Thermophilic
Chalcopyrite Bioleaching Heap Employing Mesophilic and
Thermophilic Microbes
J. Vilcaez, K. Suto and C. Inoue ............................ 70
Biological Transformation of Kimberlite Ores
M. Gericke, B. Benvie and L. Krüger ......................... 75
Bioleaching of a Zinc Sulfide Ore by Thermophilic Consortia
Isolated from Copahue Volcano
A. Giaveno and E. Donati .................................... 79
Electrochemical Noise Analysis of Chalcopyrite Carbon Paste
Electrodes by Acidithiobacillus ferrooxidans
D.G. Horta, D. Bevilaqua, H.A. Acciari, О. Garcia Jr.
and A.V. Benedetti .......................................... 83
Influence of Chelators on Iron Solubilization from Quartz and
Feldspars by Bioleaching
I. Štyriakoví ............................................... 87
Biooxidation and Cyanidation for Gold and Silver Recovery
from Acid Mine Drainage Generating Tailings (Ticapampa, Peru)
A.A. Nagy, E.D. Gock, F. Melcher, T. Atmaca, L. Hahn and
A. Schippers ................................................ 91
A View on the Organic Matter and Metalloporphyrins
Biodegradation as Characteristic Components of Black Shale
Ores
Z. Sadowski, A. Szubert, I.H. Maliszewska and E. Jazdzyk .... 95
Microbial Leaching of Metals from Printed Circuit Boards
S. Willscher, M. Katzschner, K. Jentzsch, S. Matys and
H. Pöllmann ................................................. 99
Bioleaching of a Rich-In-Carbonates Copper Ore at Alkaline pH
V. Groudeva, K. Krumova and S. Groudev ..................... 103
Cobalt and Nickel Recoveries from Laterite Tailings by
Organic and Inorganic Bioacids
O. Coto, F. Galizia, E. González, I. Hernápndez,
J. Marrero and E. Donati ................................... 107
Bioleaching of a High Iron Content Kaolin by Aspergillus
niger; The Effects of Organic Acids Biosynthesis
M. Pazouki, M.R. Hosseini, M. Ranjbar and F. Ghavipanjeh ... 111
Optimization of Kaolin Bioleaching by Aspergillus niger
M. Ranjbar, E. Aghaie, M.R. Hosseini, M. Pazouki and
F. Ghavipanjeh ............................................. 115
Application of Mesophilic Mixed Micro-Organisms for Recovery
of Valuable Metals from Spent Refinery Catalysts
D.J. Kim, D. Mishra, D.E. Ralph, J.G. Ahn and Y.H. Rhee .... 119
Biological Purification of Silicate Minerals
I. Štyriaková and M. Lovás ................................. 126
Deferrization of Kaolinic Sand by Iron Oxidizing and Iron
Reducing Bacteria
D. Kupka, M. Lovás and V. Šepelák .......................... 130
Sulfur Product Layer in Sphalerite Biooxidation: Evidences
for a Mechanism of Formation
D.M. Zapata, M.A. Márquez and D.M.H. Ossa .................. 134
Monitoring of Hydrocarbon Gaseous Compounds formed During
Bioleaching of Copper Sulphide Minerals with Mesophilic
Microorganisms
R. Luca, T. Vargas, B. Escobar and B. Townley .............. 139
Bioleaching of a Mixed Copper Dust Emanating from Copper
Smelters
F. Bakhtiari, H. Atashi, M. Zivdar and S.A. Seied Baghery .. 143
Investigation of Viscosity and Thermodynamic Properties on
the Bioleaching Solution with and without Mesophilic Bacteria
M.L. Liu, R.M. Ruan, J.K. Wen and D.Z. Wang ................ 149
Process Enhancement of Bioleaching of Covellite Concentrate
through Modification of Solution Potential
E. Zhou, J.K. Wen, R.M. Ruan and D.Z. Wang ................. 152
The Effect of Aluminium and Magnesium Sulphate on the Rate
of Ferrous Iron Oxidation by Leptospirillum ferriphilum in
Continuous Culture
T.V. Ojumu, J. Petersen and G.S. Hansford .................. 156
Kinetic Measurement of Biological Oxidation of Ferrous Iron
at Low Ferric to Ferrous Ratios in a Controlled Potential
Batch Reactor
Т.К. Kazadi and J. Petersen ................................ 160
Short Abstracts
Bioleaching of Multimetal Black Shale by Thermophilic
Micro-Organisms
J. Langwaldt ............................................... 167
Effect of Temperature on Bioleaching of Chalcopyrite
Concentrates containing High Concentrations of Silver:
Opposite Rules Apply
L. Yajie, N. Okibe and D.B. Johnson ........................ 168
Microbial Populations in a 110 Ton-Scale Column for the
Recovery of Metals from Black Schist Ores
K.B. Hallberg, D.B. Johnson, J. Langwaldt and C. Joulian ... 170
Combined Test for Bioleaching Activities
T. Rohwerder and W. Sand ................................... 171
Study on Selective Depression of Pyrite during Copper
Bioleaching
R.M. Ruan, G.Y. Zhou and J.K. Wen .......................... 172
III. Environmental Problems
(edited by S. Willscher)
Keynote Paper
Biogeochemical Processes in Mine Tailings with Special Focus
on Marine Shore Tailings Deposits and their Remediation
B. Dold .................................................... 177
Extended Abstracts
Modelling of Contaminant Release from Mining Waste Deposits
L. Moreno .................................................. 189
Environmental Toxicity Testing in the Risk Assessment of
a Metal Contaminated Abandoned Mining Site in Hungary
K. Gruiz, E. Vaszita and Z. Siki ........................... 193
Application of Sulfate Reduction for the Biological
Conversion of Anglesite to Galena
A. Wolthoorn, S. Kuitert, H. Dijkman and J.L. Huisman ...... 197
First Evidence of Active Sulphate Reduction in a Lignite
Mine Dump Site at Low pH Values - the Plessa Site
A. Storch, K. Tröger, N. Hoth and K. Knöller ............... 201
Treatment of Uranium-Containing Drainage Water in a Pilot-
Scale Plant by Means of Microbial Reduction
F. Glombitza, S. Kutschke, G. Kiessig and A. Zellmer ....... 205
Development and Potential Application of a Method for the
Prediction and Assessment of the Seepage Water Quality
F. Glombitza, J. Pinka, S. Kutschke, E. Janneck and
R. Kahnt ................................................... 209
Accumulation of Heavy Metals in Anaerobic Sludge and its
Disposal by Landspreading
A. Arora and S. Saxena ..................................... 213
Microbial and Geochemical Characterization of an Acid-
Generating Hard Coal Tailings Dump in Saxonia/ Germany
S. Willscher, S. Starke, M. Katzschner and M. Felix ........ 217
Environmental Risk Management of an Abandoned Mining Site in
Hungary
K. Gruiz, E. Vaszita, Z. Siki and V. Feigl ................. 221
New Xochiquetzalli Method for Sulfide Ion Determination in
Aqueous Media. Application to Tailings Analysis.
L. Juárez-García and R.E. Rivera-Santillán ................. 226
A Study of the Operating Parameters of a Sulphate-Reducing
Fixed-Bed Reactor for the Treatment of Metal-Bearing
Wastewater
P. Kousi, E. Remoudaki, A. Hatzikioseyian and M. Tsezos .... 230
Short Abstracts
Iron Isotope Fractionation by Biogeochemical Processes in
Mine Tailings
R.B. Herbert and A. Schippers .............................. 237
Biodegradation of Organic Matter and Release of Heavy Metals
from the Copper Bearing Black Shale of Fore Sudetic
Monocline (Poland)
R. Matlakowska and A. Sklodowska ........................... 238
IV. (Bio)-Remediation
(edited by W. Sand)
Keynote Papers
The Wismut Uranium Mining Rehabilitation Project Running for
15 Years - Lessons Learned and Achievements
D.M. Hagen ................................................. 243
Bioremediation of Acid Mine Drainage in an Uranium Deposit
S. Groudev, P. Georgiev, I. Spasova and M. Nicolova ........ 248
Extended Abstracts
Sustainable Approach for the Immobilization of Metals in the
Saturated Zone: In Situ Bioprecipitation
K. Vanbroekhoven, S. Van Roy, L. Diels, J. Gemoets,
P. Verkaeren, L. Zeuwts, K. Feyaerts and F. van den
Broeck ..................................................... 261
Development of a Novel Biological System for Removing
Manganese from Contaminated Waters
R. Mariner, D.B. Johnson and K.B. Hallberg ................. 267
In-Lake Bioreactors for the Treatment of Acid Mine Water in
Pit Lakes
V. Preuss, M. Horn, M. Koschorreck, G. Luther,
K. Wendt-Potthoff and W. Geller ............................ 271
Biosorption as Main Uranium Accumulation Mechanism in
Wetlands
A. Schöner, G. Büchel and M. Sauter ........................ 275
Integrated System to Biological Solubilization and
Precipitation of Heavy Metals
G. Cabrera, J.M. Gómez and D. Cantero ...................... 279
Remediation of Acid Mine Drainage by Means of Biological and
Chemical Methods
A. Luptakova, T. Spaldon and M. Balintova .................. 283
Bioremediation In Situ of an Alkaline Soil Polluted with
Heavy Metals
V. Groudeva, A. Doycheva, K. Krumova and S. Groudev ........ 287
Mine Waste Stabilization with Biosludge and Ca Carbonate
Residues: Column Experiments
R.B. Herbert, L. Höckert, M. von Brömssen, H. Friis and
G. Jacks ................................................... 291
Application Possibility of Bentonite and Zeolite in
Bioremediation
K. Jablonovská and I. Štyriaková ........................... 295
Biohydrometallurgical Processing of Flotation Tailings from
Different Copper Mills
M.G. Sagdieva, S.I. Borminskiy, Z.E. Rakhmatullaeva,
A.K. Tonkikh, K.S. Sanakulov and B. Scott .................. 299
Enhancing Reductive Processes by Autochthonic Sulphate-
Reducing Bacteria in Lignite Overburden Dumps
A. Simon, N. Hoth and M. Dilbat ............................ 304
Bioremediation of Dredged Sediments Polluted by Heavy Metals
F. Beolchini, S. Ubaldini, B. Passariello, N. Gül,
D. Türe, F. Vegliò, R. Danovaro and A. Dell'Anno .......... 307
Laboratory Investigations on the Interactions of Soil, Water
and Microorganisms with Manganese
C. Lorenz, D. Merten, G. Haferburg and G. Büchel ........... 311
Chemical Stabilisation Combined with Phytostabilisation
Applied to Mine Waste Contaminated Soils in Hungary
V. Feigl, Á. Atkári, A. Anton and K. Gruiz ................. 315
Regulation of PCE-Dechlorinating Activity by Electrochemical
Cultivation
S.I. Hirano, N. Matsumoto and N. Ohmura .................... 319
Short Abstracts
Sulfate Reduction under Acidic Conditions in High Rate
Bioreactor Systems for Treatment of Mining and Metallurgical
Waste and Process Water
M.F.M. Bijmans, C.J.N. Buisman and P.N.L. Lens ............. 324
Hydrolysed Cellulose Material as Sulfate Reduction Electron
Donor to Treat Metal- and Sulfate Containing Waste Water
A.M. Lakaniemi, L.M. Nevatalo, A.H. Kaksonen and
J.A. Puhakka ............................................... 326
V. Interfacial Processes
(edited by W. Sand)
Keynote Paper
Recent Advances in Electron Transfer Between Biofilms and
Metals
A. Bergel .................................................. 329
Extended Abstracts
Investigations on the Sorting of Very Fine Particles by
Biocoagulation
H.Z. Kuyumcu, J. Pinka and T. Bielig ....................... 337
Effect of LPS Removal on Electrophoretic Softness of
Acidithiobacillus ferrooxidans Cells
M.N. Chandraprabha, J.M. Modak and K.A. Natarajan .......... 341
Is the Quorum Sensing Type AI-1 System of Acidithiobacillus
ferrooxidans Involved in its Attachment to Mineral Surfaces?
L.M. Ruiz, A. Gonzalez, M. Frezza, L. Soulere, Y. Queneau,
A. Doutheau, T. Rohwerder, W. Sand, C.A. Jerez and
N. Guiliani ................................................ 345
Pyrite Surface Alteration of Synthetic Single Crystals as
Effect of Microbial Activity and Crystallographic
Orientation
K. Etzel, H. Huber, R. Rachel, G. Schmalz, M. Thomm and
W. Depmeier ................................................ 350
The Catalytic Influence of Sulfolobus metallicus in the
Bioleaching of Chalcopyrite: Role of Attached and Planktonic
Population
V. Gautier, B. Escobar and T. Vargas ....................... 354
Bioflotation of a Black Shale Copper Ore
I. Spasova, M. Nicolova and S. Groudev ..................... 358
Surface Hydrophobicity of an Acidophilic Heterotrophic
Bacterium of Mine Origin under Metal Stress
K. Pakshirajan ............................................. 362
Selective Coagulation in Chalcopyrite/Pyrite Mineral System
Using Acid Uhiobacillus Group Bacteria
A. Vilinska, K. Hanumantha Rao and K.S.E. Forssberg ........ 366
Combined Atomic Force and Fluorescence Microscopy of Biofilms
formed by Leaching Bacteria on Opaque Substrata
S. Mangold, K. Harneit and W. Sand ......................... 371
Novel Steel Corrosion Protection by Microbial Extracellular
Polymeric Substances (EPS) -Biofilm-Induced Corrosion
Inhibition
M. Grooters, K. Harneit, M. Wöllbrink, W. Sand,
R. Stadler and W. Fürbeth .................................. 375
Inhibition of Microbiologically Influenced Corrosion of Mild
Steel and Stainless Steel 316 by an Organic Inhibitor
X.X. Sheng, Y.P. Ting and S.O. Pehkonen .................... 379
Short Abstracts
Influence of Growth Substrate and Attachment Substratum on
EPS and Biofilm Formation by Acidithiobacillus ferrooxidans
K. Harneit and W. Sand ..................................... 385
Study of the Attachment Behaviour of Different Strains of
Acidithiobacillus spp. to Pyrite
K.Z. Mafanya, T. Rohwerder and W. Sand ..................... 386
VI. Molecular and Microbiology
(edited by A. Schippers)
Keynote Paper
Biomining in the Post-Genomic Age: Advances and
Perspectives
C.A. Jerez ................................................. 389
Extended Abstracts
Concentrate Mineralogy Dictates the Composition of
Bioleaching Microbial Consortia
D.B. Johnson, L. Yajie, N. Okibe, K. Coupland and
K.B. Hallberg .............................................. 403
Geographical Distribution and Diversity of Moderately
Thermophilic Members of the Thermoplasmatales
R. Hawkes, G. O'Hara, P. Franzmann and J.J. Plumb .......... 405
Microbial Ecology of Leptospirillum spp. in Rio Tinto,
a Model of Interest to Biohydrometallurgy
A. Garcнa-Moyano, E. González-Toril, M. Moreno-Paz,
V. Parro and R. Amils ...................................... 409
Molecular Assessment of Microbial Diversity and Community
Structure at Uranium Mines of Jaduguda, India
P. Sar, P.K. Dhal, E. Islam and S.K. Kazy .................. 413
Related Arsenic Resistance Transposons Occur in
Leptospirilli Isolated from Different Continents
A. Kloppers, S. Deane and D.E. Rawlings .................... 417
Interactions of Acidithiobacillus ferrooxidans with Heavy
Metals, Various Forms of Arsenic and Pyrite
S.R. Dave and K.H. Gupta ................................... 423
Regulation of the Arsenic Oxidation Encoding Genes of
a Moderately Acidophilic, Facultative Chemolithoautotrophic
Thiomonas sp.
D. Slyemi, J. Ratouchniak and V. Bonnefoy .................. 427
Ferrous Iron Oxidation by Salt-Tolerant "Thiobacillus
prosperus"
C.S. Davis-Belmar, J.L.C. Nicolle and P.R. Norris .......... 431
Differential Genetic Expression in Heap Bioleaching of
Low-Grade Copper Sulphide Ore at Escondida Mine, Chile
P. Galleguillos, V. Zepeda, F. Galleguillos, D. Castillo,
N. Guiliani, F. Remonsellez, E. Ortiz and C. Demergasso .... 435
Insights into the Metabolism and Ecophysiology of Three
Acidithiobacilli by Comparative Genome Analysis
J. Valdйs, I. Pedroso, R. Quatrini, K.B. Hallberg,
P.D.T. Valenzuela and D.S. Holmes .......................... 439
Involvement of Iron Oxidation Enzyme System in Sulfur
Oxidation of Acidithiobacillus ferrooxidans ATCC 23270
T.M. Taha, T. Kanao, F. Takeuchi and T. Sugio .............. 443
The Effect of Total Iron Concentration and Iron Speciation
on the Rate of Ferrous Iron Oxidation Kinetics of
Leptospirillum ferriphilum in Continuous Tank Systems
J. Petersen and T.V. Ojumu ................................. 447
Identification, Some Characterizations and Bioleaching
Application of an Iron-Oxidizing Bacterium,
Acidithiobacillus ferrooxidans KLipis-3-1 Isolated from
Malaysia
S.C. Yee, C.P. Lim and K.Y. Ng ............................. 453
Quantification of Biomining Microorganisms Using
Quantitative Real-Time Polymerase Chain Reaction
C.M. Zammit, L.A. Mutch, H.R. Watling and E.L.J. Watkin .... 457
Chalcopyrite and Bornite Differentially Affect Protein
Synthesis in Planktonic Cells of Acidithiobacillus
ferrooxidans
A.P. Felício, E. de Oliveira, M.A. Odean, O. Garcia Jr.,
M.C Bertolini, L.F.C. Ferraz, F.C. Reis, L.M.M. Ottoboni
and M.T.M. Novo ............................................ 461
Temperature Effects on the Iron Oxidation Kinetics of
a Leptospirillum ferriphilum Dominated Culture at pH Below
One
B. Özkaya, P. Nurmi, E. Sahinkaya, A.H. Kaksonen and
J.A. Puhakka ............................................... 465
Metabolism Peculiarities of Bacteria of the Genus
Sulfobacillus
A.E. Zhuravleva, L.A. Tsaplina, A.D. Ismailov,
L.M. Zakharchuk and G.I. Karavaiko ......................... 469
Isolation, Characterization and Phylogenetic Analysis of
Acidiphilium-hike Bacteria from Acid Mine Drainage
Y.F. Zhang, Y. Yang, J.S. Liu and G.Z. Qiu ................. 473
Bacterial Oxidation of Elemental Sulfur: Changes in
Oxidation Kinetics
M. Mandl, B. Pokorna and P. Gavlasova ...................... 477
Quantification of Microorganisms Involved in Cemented Layer
Formation in Sulfidic Mine Waste Tailings (Freiberg, Saxony,
Germany)
D. Коек, T. Graupner, D. Rammlmair and A. Schippers ........ 481
Analysis of the Microbial Community in the Leachate
Collected from an Experimental Bioleaching Column by Cloning
and RFLP
L.A. Mutch, E.L.J. Watkin and H.R. Watling ................. 485
The Diversity of Benthic Microorganisms in Acidic Mine Lake
Sediments
H.A. Pham, C.E. Oldham and J.J. Plumb ...................... 489
Microbial Diversity in an Iron Oxidation Tank of an AMD
Treatment Plant at an Abandoned Sulphur Mine
K. Suto, H. Bacosa, C. Inoue and E. Matsushima ............. 493
Reduction of Soluble and Solid Ferric Iron by Acidiphilium
SJH
A. Vašková and D. Kupka .................................... 497
Development of an Extremophilic Iron Oxidizing Consortium
and a Fixed Film Bioreactor for Generation of Ferric
Lixivient
S.R. Dave, T.J. Shah and D.R. Tipre ........................ 501
Enrichment of Chromium-Reducing Bacteria Using Potential-
Controlled Electrochemical Cultivation
N. Matsumoto, S.I. Hirano and N. Ohmura ....................... 505
Research on isc Operon in Acidiphiobacillus ferrooxidans ATCC
23270
J.S. Liu, Y.F. Zhang, M.M. Geng, J. Zeng and G.Z. Qiu ...... 509
Effect of Sulphate Concentration on the Community Structure
and Activity of Sulphate Reducing Bacteria
O.O. Oyekola, R.P. van Hille and S.T.L. Harrison ........... 513
Kinetics of Ferrous Iron Oxidation by Moderately
Thermophilic Microorganisms
P.S. Pina, V.A. Leão, C.A. Silva, S.N. Medrício and
J. Frenay .................................................. 517
High-Level Resistance to Cobalt and Nickel in Cuban Serratia
marcescens Strains Isolated from Serpentine Deposits
J. Marrero, G. Auling, O. Coto and D. Nies ................. 521
Microbial Diversity in a Pilot Plant for Producing Iron
Hydroxysulfates
E. Heinzel, S. Hedrich, J. Seifert and M. Schlömann ........ 527
Iron Homeostasis Strategies in Acidophilic Iron Oxidizers:
Comparative Genomic Analyses
R. Quatrini, V. Martinez, H. Osorio, F.A. Veloso,
I. Pedroso, J. Valdés, E. Jedlicki and D.S. Holmes ......... 531
Discovery of Small Regulatory RNAs Extends Our Understanding
of Gene Regulation in the Acidithiobacillus Genus
A. Shmaryahu and D.S. Holmes ............................... 535
Monitoring of Microbial Community Inhabiting a Low-Grade
Copper Sulphide Ore by Quantitative Real-Time PCR Analysis
of 16S rRNA Genes
F. Remonsellez, F. Galleguillos, S. Janse van Rensburg,
G.F. Rautenbach, P. Galleguillos, D. Castillo and
C. Demergasso .............................................. 539
Bacterial Activity at Low Temperature in Cultures Derived
from a Low-Grade Copper Sulphide Bioleaching Heap at the
Escondida Mine, Chile
V. Zepeda, F. Galleguillos, D. Castillo, M. Lastra and
C. Demergasso .............................................. 543
Isolation and Molecular Characterization of Sulfate Reducing
Bacteria in a Brazilian Acid Mine Drainage
L.R. Rampinelli, R.D. Azevedo, R. Guerra-Sá,
M.C. Teixeira and V.A. Leao ................................ 547
C-di-GMP Pathway in Acidithiobacillus ferrooxidans: Analysis
of Putative Diguanylate Cyclases (DGCs) and
Phosphodiesterases (PDEs) Bifunctional Proteins
L.M. Ruiz, W. Sand, C.A. Jerez and N. Guiliani ............. 551
Searching for Useful Bacteria on Chalcopyrite Leaching from
Japanese Abandoned Mines
S.J. Joe, M. Sakoda, T. Chida, Y. Kida, H. Nakamura and
M. Tamura .................................................. 557
Comparative Bioreduction of Fe(III) with Geobacter
metallireducens and Bacillus infernus
J. Crespo, J.A. Muñoz, F. González, M.L. Blázquez and
A. Ballester ............................................... 561
The Use of CARD-FISH to Evaluate the Quantitative Microbial
Ecology Involved in the Continuous Bioleaching of
a Cobaltiferrous Pyrite
E. Díaz, E. González-Toril, С. Joulian and R. Amils ........ 565
Short Abstracts
The Effect of Multiple Stresses on Sulfolobus-like Cultures
in Bioleaching Systems: Super-Imposing Stress Responses to
Metal Concentration and Solids Loading
S.T.L. Harrison, N.J. Coram-Uliana, K. Ntshabele and
R.P. van Hille ............................................. 571
A New Acid-Stable, Fe-Oxidizing/O2-Reducing Supercomplex
Spanning Both Inner and Outer Membranes, Isolated from the
Extremophile Acidithiobacillus
C. Castelle, M. Guiral and M.T. Giudici-Orticoni ........... 572
Transporter Protein Genes are Up-Regulated by Covellite in
Acidithiobacillus thiooxidans
F. C. Reis, D.J. Madureira, C. Carlos, D.M.H. Ossa,
S.F.S. Tada and L.M.M. Ottoboni ............................ 573
Up- and Down-Regulated Genes in Acidithiobacillus
ferrooxidans Exposed to Chalcopyrite
L.F.C. Ferraz, F.C. Reis, S.F.S. Tada, D.M.H. Ossa,
A.P. Felнcio, M.T.M. Novo, O. Garcia Jr. and
L.M.M. Ottoboni ............................................ 574
Isolation and Characterization of a Psychrotolerant
Arsenite-Oxidizing Bacterium from a Gold Mine in Zloty
Stok, Poland
L. Drewniak and A. Sklodowska .............................. 575
Arsenic Hypertolerant Bacteria Isolated from Gold Mine Rocks
Biofilms
L. Drewniak, A. Styczek and A. Sklodowska .................. 576
Microbial Communities in Acid Mine Water from Two Different
Copper Mines in China
X.H. Xie, S.M. Xiao, Z.G. He, J.S. Liu and G.Z. Qiu ........ 577
Iron Oxidation and Bioleaching Potential at Low Temperatures
M. Dopson, D. Kupka, A.K. Halinen, N. Rahunen, B. Özkaya,
E. Sahinkaya, O.I. Rzhepishevska, A.H. Kaksonen,
O.V. Karnachuk, O.H. Tuovinen and J.A. Puhakka ............. 578
Microbial Community of the Talvivaara Demonstration-Scale
Bioheap
A.K. Halinen, N. Rahunen, K. Määttä, A.H. Kaksonen,
M. Riekkola-Vanhanen and J.A. Puhakka ...................... 579
Isolation and Characterisation of Microorganisms from Copper
Bearing Black Shale of Lubin Mine (Poland)
R. Matlakowska, K.B. Hallberg and A. Sklodowska ............ 580
Analysis of the Microbial Community in an Acid Ground Water
Characterized by Sulfate Reduction Activity
K. Gumnior, N. Hoth, M. Schlömann and J. Seifert ........... 581
Characterization of New Iron Oxidizing Bacteria from an Acid
Mine Water Treatment Plant
S. Hedrich, E. Heinzel, J. Seifert, K.B. Hallberg,
D.B. Johnson and M. Schlömann .............................. 582
Elemental Sulfur Oxidation in Acidiphilium spp.
T. Rohwerder, C. Janosch and W. Sand ....................... 583
Sulfur Oxidation and Coupled Iron Reduction at Low
Temperatures
D. Kupka, M. Dopson and O.H. Tuovinen ...................... 584
Comparison of Microbiological Populations of Mineral Heaps
and Mine Wastes of Differing Ages in Active and Abandoned
Copper Mines
C.G. Bryan, K.B. Hallberg and D.B. Johnson ................. 585
Proteomic Studies in Acidthiobacillus ferrooxidans Cells
Induced by Synthetic Acyl-Homoserine Lactones
A. Gonzalez, M.J. Gallardo, M. Frezza, L. Soulere,
Y. Queneau, A. Doutheau, C.A. Jerez and N. Guiliani ......... 586
VII. Biosorption and -Accumulation
(edited by S. Willscher)
Keynote Paper
Biological Removal of Ions: Principles and Applications
M. Tsezos .................................................. 589
Extended Abstracts
Lead and Gold Removal Using Sugar-Beet Pectin Gels with and
without Immobilized Fucus Vesiculosus
Y.N. Mata, E. Torres, M.L. Blázquez, A. Ballester,
F. González and J.A. Muñoz ................................. 599
A Novel Hydrogenation and Hydrogenolysis Catalyst
Using Palladized Biomass of Gram-negative and
Gram-positive Bacteria
N.J. Creamer, LP. Mikheenko, K. Deplanche, P. Yong,
J. Wood, K. Pollmann, S. Selenska-Pobell and L.E. Macaskie .. 603
Sorption of Co Ions on Biogenic Mn Oxides Produced by
a Mn-Oxidizing Fungus, Paraconiothyrium sp.-like Strain
K. Sasaki, M. Matsuda, T. Urata, T. Hirajima and
H. Konno ................................................... 607
The Role of Carotenoid Pigments in Cr(VI) Tolerance,
Biosorption and Bioaccumulation by Rhodotorula mucilaginosa
UCM Y-1776 and its Mutants
O.G. Mameeva, T.P. Kasatkina and V.S. Podgorsky ............ 611
Biosorption of Heavy Metals by Pseudomonas aeruginosa
Isolated from a Petroleum Contaminated Site
R.M. Pérez, A. Abalos, J.M. Gómez and D. Cantero ........... 615
Waste Biomass Characterization and Metal-Biomass Interactions Study
L. Svecova, M. Kubal and E. Guibal ......................... 619
Waste Fungal Biomass for Mercury Biosorption - Column Studies
L. Svecova, M. Kubal and E. Guibal ......................... 623
Removal of Copper and Uranium from Contaminated Waters in
Biosorption Columns
K. Steudel, G. Horak, S. Willscher, W. Pompe and
P. Werner .................................................. 627
Sorption of Copper Ions by Biogenic Iron Sulphides
A. Luptakova and E. Macingova .............................. 631
Mercury Sorption on Chitosan
K. Campos, E. Guibal, F. Peirano, M. Ly and H. Maldonado ... 635
Heavy Metal Tolerance and Copper Uptake in Yeast Isolated
from Patagonia/Argentina
L. Lavalle, M. Portillo, P. Chiacchiarini and E. Donati .... 639
Characterization of Cellulosic Biomass Loaded with Iron and
Arsenic Using High Energy X-Rays
G. Cordeiro Silva, R. Pinto de Carvalho, M.S.S. Dantas
and V.S.T. Ciminelli ....................................... 643
Biorecovery of Gold from Jewellery Wastes by Escherichia
Coli and Biomanufacture of Active Au-Nanomaterial
K. Deplanche, G.A. Attard and L.E. Macaskie ................ 647
Biorecovery of Platinum Group Metals from Secondary Sources
A.J. Murray, I.P. Mikheenko, E. Goralska, N.A. Rowson and
L.E. Macaskie .............................................. 651
A Novel Fuel Cell Catalyst for Clean Energy Production Based
on a Bionanocatalyst
P. Yong, I.P. Mikheenko and L.E. Macaskie .................. 655
Author Index .................................................. 659
Keyword Index ................................................. 665
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