Preасе .......................................................... v
Contributors ................................................... iх
1 Interfollicular Epidermal Stem Cells: Boosting and
Rescuing from Adult Skin ..................................... 1
Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, and
Alexandra P. Marques
2 Isolation and Cultivation of Human Scalp Interfollicular
Epidermal Stem Cells ........................................ 11
Longmei Zhao and Basil M. Hantash
3 Isolation of Hair Follicle Bulge Stem Cells from YFP-
Expressing Reporter Mice .................................... 21
Kerry-Ann Nakrieko, Timothy S. Irvine, and Lina Dagnino
4 Analysis of Bulge Stem Cells from the Epidermis
Using Flow Cytometry ........................................ 33
Rehan M. Villani, Mehmet Deniz Akyuz, Michaela T. Niessen,
and Carien M. Niessen
5 Lineage Tracing of Hair Follicle Stem Cells in Epidermal
Whole Mounts ................................................ 45
Monika Petersson, Daniela Frances, and Catherin Niemann
6 Isolation and Characterization of Cutaneous Epithelial
Stem Cells .................................................. 61
Uffe B. Jensen, Soosan Ghazizadeh, and David M. Owens
7 Identification and Analysis of Epidermal Stem Cells
from Primary Mouse Keratinocytes ............................ 71
Youliang Wang and Xiao Yang
8 Monitoring the Cycling Activity of Cultured Human
Keratinocytes Using a CFSE-Based Dye Tracking Approach ...... 83
Loubna Chadli, Emmanuelle Cadio, Pierre Vaigot, Michèle
T. Martin, and Nicolas O. Fortunel
9 Quantification of Cyclobutane Pyrimidine Dimers in Human
Epidermal Stem Cells ........................................ 99
M. Ruetze, S. Gallinat, H. Wenck, and A. Knott
10 Analysis of Gene Expression in Skin Using Laser Capture
Microdissection ............................................ 109
Briana Lee, Mikhail Geyfman, Bogi Andersen, and Xing Dai
11 Qualitatively Monitoring Binding and Expression of the
Transcription Factor Spl as a Useful Tool to Evaluate
the Reliability of Primary Cultured Epithelial Stem Cells
in Tissue Reconstruction ................................... 119
Manon Gaudreault, Danielle Larouche, Lucie Germain, and
Sylvain L. Guérin
12 Genetic Manipulation of Keratinocyte Stem Cells with
Lentiviral Vectors ......................................... 143
Masahito Yasuda, David J. Claypool, Erika Guevara,
Dennis R. Roop, and Jiang Chen
13 Using 3D Culture to Investigate the Role of Mechanical
Signaling in Keratinocyte Stem Cells ....................... 153
Lee Wallace and Julia Reichelt
14 In Vivo Transplantation Assay at Limiting Dilution to
Identify the Intrinsic Tissue Reconstitutive Capacity of
Keratinocyte Stem Cells and Their Progeny .................. 165
Holder Schlüter and Pritinder Kaur
15 Melanoblasts as Multipotent Cells in Murine Skin ........... 183
Tsutomu Motohashi and Takahiro Kunisada
16 Generation of Human Melanocytes from Induced Pluripotent
Stem Cells ................................................. 193
Shigeki Ohta, Yoichi Imaizumi, Wado Akamatsu, Hideyuki
Okano, and Yutaka Kawakami
17 Cellular Populations Isolated from Newborn Mouse Skin
Including Mesenchymal Stem Cells ........................... 217
Lauren Kimlin and Victoria Virador
18 Isolation, Characterization, and Differentiation of Human
Multipotent Dermal Stem Cells .............................. 235
Ling Li, Mizubo Fukunaga-Kalabis, and Meenhard Herlyn
19 Isolation and Differentiation of Hair Follicle-Derived
Dermal Precursors .......................................... 247
Andrew Hagner and Jeff Biernaskie
20 Isolation of Mesenchymal Stem Cells from Human Dermis ...... 265
Tsutomu Soma, Jiro Kishimoto, and David Fisher
21 Skin-Derived Mesenchymal Stem Cells: Isolation, Culture,
and Characterization ....................................... 275
M. Orciani and R. Di Primio
22 Isolation and Establishment of Hair Follicle Dermal
Papilla Cell Cultures ...................................... 285
Karl Gledhill, Aaron Gardner, and Colin A.B. Jahoda
23 Enrichment of Oral Mucosa and Skin Keratinocyte
Progenitor/Stem Cells ...................................... 293
Kenji Izumi, Cynthia L. Marcelo, and Stephen E. Feinberg
24 Skin Stem Cells ............................................ 305
Keita Inoue and Kotaro Yoshimura
Index ......................................................... 315
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