Preface ......................................................... v
Contributors ................................................... ix
Section I: Reminiscences
1 On the Discovery of Precursor Processing ..................... 3
Donald F. Steiner
2 The Prohormone Theory and the Proprotein Convertases: It
Is All About Serendipity .................................... 13
Michel Chrétien
Section II: Biochemistry and Cell Biology
3 The Proprotein Convertases, 20 Years Later .................. 23
Nabil G. Seidah
4 Insights from Bacterial Subtilases into the Mechanisms
of Intramolecular Chaperone-Mediated Activation of Furin .... 59
Ujwal Shinde and Gary Thomas
5 The Novel Role of Cathepsin L for Neuropeptide Production
Illustrated by Research Strategies in Chemical Biology
with Protease Gene Knockout and Expression ................. 107
Lydiane Funkelstein and Vivian Hook
6 In Vitro Assay for Protease Activity of Proprotein
Convertase Subtilisin Kexins (PCSKs): An Overall Review
of Existing and New Methodologies .......................... 127
Ajoy Basak, Andrew Chen, Swapan Majumdar, and Heather
Palmer Smith
7 Inhibitor Screening of Proprotein Convertases Using
Positional Scanning Libraries .............................. 155
Iris Lindberg and Jon R. Appel
8 Analyses of PCSK9 Post-translational Modifications Using
Time-of-Flight Mass Spectrometry ........................... 167
Thilina Dewpura and Janice Mayne
Section III: Molecular Biology and Genetics
9 The Molecular Biology of Furin-Like Proprotein Convertases
in Vascular Remodelling .................................... 191
Philipp Stawowy and Kai Kappert
10 Identification of the Myosin Heavy Polypeptide 9 as
a Downstream Effector of the Proprotein Convertases in
the Human Colon Carcinoma HT-29 Cells ...................... 207
Nathalie Scamuffa, Peter Metrakos, Fabien Calvo, and
Abdel-Majid Khatib
11 Regulation of 7B2 mRNA Translation: Dissecting the Role
of Its 5'-Untranslated Region .............................. 217
Haidy Tadros, Gunther Schmidt, Francine Sirois, and
Majambu Mbikay
12 Analysis of Epigenetic Alterations to Proprotein
Convertase Genes in Disease ................................ 231
Yang Xin Fu and Mark W. Nachtigal
13 Genetic and Functional Characterization of PCSK1 ........... 247
Hélène Choquet, Pieter Stijnen, and John W.M. Creemers
Section IV: Experimental Models
14 Prohormone Processing in Zebrafish ......................... 257
Michael G. Morash, Kelly Soanes, and Yonnes Anini
15 Use of Zebrafish and Knockdown Technology to Define
Proprotein Convertase Activity ............................. 273
Babykumari P. Chitramuthu and Hugh P.J. Bennett
16 Characterization of Impaired Processing of Neuropeptides
in the Brains of Endoprotease Knockout Mice ................ 297
Margery C. Beinfeld
17 Quantitative Peptidomics of Mice Lacking Peptide-
Processing Enzymes ......................................... 307
Jonathan Wardman and Lloyd D. Fricker
18 A Proteomic Protocol to Identify Physiological
Substrates of Pro-protein Convertases ...................... 325
Guiying Nie and Andrew N. Stephens
19 Neurophenotyping Genetically Modified Mice for Social
Behavior ................................................... 343
Ramona M. Rodriguiz, Jennifer S. Colvin, and William
C. Wetsel
Index ......................................................... 365
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