Preface ........................................................ ix
Acknowledgments ................................................ xi
Editors ...................................................... xiii
Contributors ................................................... xv
Part I Devices for Chromosome Handling
Chapter 1 Mechanical Approaches to Elucidate Mechanisms of
Chromosome Condensation at the Nano- and Microlevels ............ 1
Kazushige Kawabata, Keisuke Nomura, Kensuke Ikeda, Osamu
Hoshi, Daisuke Fukushi, Hisashi Haga and Tatsuo Ushiki
Chapter 2 Development of Novel AFM Probes for Chromosome
Manipulation ................................................... 15
Masato Saito, Gen Hashiguchi and Eiichi Tamiya
Chapter 3 Microchamber Array-Based Sequence-Specific DNA
Detection from a Single Chromosome via Trace Volume PCR ........ 31
Masato Saito and Eiichi Tamiya
Chapter 4 On-Chip Chromosome Sorter Using Electric and
Magnetic Fields ................................................ 43
Takahito Inoue, Yasuyuki Fujita, Susumu Uchiyama, Tomoyuki
Doi, Kiichi Fukui and Hiroshi Yokoyama
Part II Visualization of Chromosomes at the Nano- and
Microlevels
Chapter 5 Fluorescence Microscopy for Analysis of
Chromosome Dynamics ............................................ 53
Sachihiro Matsunaga
Chapter 6 Fluorescence In Situ Hybridization (FISH) as
a Tool for Comparative Genomics: Application of FISH to
Studies of Chromosome Evolution in Vertebrates ................. 63
Yoichi Matsuda, Mami Shibusawa, Kazumi Matsubara and Chizuko
Nishida-Umehara
Chapter 7 Immunocytochemistry for Analyzing Chromosomes ....... 81
Osamu Hoshi, Toru Hirota, Eiji Kimura, Nae Komatsubara and
Tatsuo Ushiki
Chapter 8 Transmission and Scanning Electron Microscopy
of Mammalian Metaphase Chromosomes ............................. 93
Sumire Inaga, Keiichi Tanaka and Tatsuo Ushiki
Chapter 9 Atomic Force Microscopy of Human Chromosomes in
Relation to Their Higher-Order Structure ...................... 105
Osamu Hoshi, Daisuke Fukushi and Tatsuo Ushiki
Chapter 10 Mechanism of Higher-Order Chromatin Folding
Revealed by AFM Observation of In Vitro Reconstituted
Chromatin ..................................................... 119
Kohji Hizume, Toshiro Kobori, Shige H. Yoshimura and Kunio
Takeyasu
Chapter 11 Scanning Near-Field Optical/Atomic Force
Microscopy as a Tool for Simultaneous Specification of
Chromosome Topography and Particular Gene Location on the
Nanometer Scale ............................................... 137
Motoharu Shichiri, Tomoyuki Yoshino, Daisuke Fukushi, Shoji
Hagiwara, Kohei Akazawa, Toshio Ohtani and Shigeru Sugiyama
Part III Chromosomes as Nanomaterials
Chapter 12 Isolation of Human and Plant Chromosomes as
Nanomaterials ................................................. 155
Susumu Uchiyama, Tomoyuki Doi and Kiichi Fukui
Chapter 13 Proteome Analysis of Human Metaphase Chromosomes ... 167
Kiichi Fukui and Susumu Uchiyama
Chapter 14 Antipeptide Antibodies for Examining the
Conformation and Molecular Assembly of an Intracellular
Protein ...................................................... 189
Masatoshi Nakagawa, Nobuko Ohmido, Katsumi Ishikawa,
Susumu Uchiyama, Kiichi Fukui and Takachika Azuma
Chapter 15 Structure and Interactions of the Imitation
SWI-Type Chromatin-Remodeling Complex, ATP-Dependent
Chromatin-Assembly Factor ..................................... 203
Tadayasu Ohkubo, Mariana Petkova-Andonova, Yukiko Kojima,
Shota Nakamura, Hiroko Fujita, Yoshinori Nishi, Hiroaki
Nakano, Susumu Uchiyama, Kiichi Fukui and Yuji Kobayashi
Chapter 16 Dynamic and Functional Analysis of Chromosomal
Proteins ...................................................... 217
Nobuko Ohmido, Susumu Uchiyama, Sachihiro Matsunaga,
Masatoshi Nakagawa, Takachika Azuma and Kiichi Fukui
Part IV Informatics of Chromosome Imaging
Chapter 17 Development of a Sustainable Chromosome Imaging
Database ...................................................... 231
Kenji Taniguchi, Chiharu Mitsueda, Naho Okumura and Wakako
Miyazaki
Chapter 18 Image Database and Image Analysis of Chromosome
Information ................................................... 245
Shin-ichi Toyabe, Takayuki Matsuto, Tatsuo Ushiki and Kohei
Akazawa
Index ......................................................... 259
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