PCR detection of microbial pathogens / ed. by M.Wilks. - 2nd ed. - New York: Humana Press, 2013. - xi, 317 p.: ill. - (Methods in Molecular Biology; 943) (Springer protocols). - Incl. bibl. ref., ind. - ISBN 978-1-60327-352-7; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 Real-Time Quantitative PCR, Pathogen Detection and MIQE ...... 1 Gemma Johnson, Tania Nolan, and Stephen A. Bustin 2 Overcoming Inhibition in Real-Time Diagnostic PCR ........... 17 Johannes Hedman and Peter Bådström 3 Quality in the Molecular Microbiology Laboratory ............ 49 Paul S. Wallace and William G. MacKay 4 Pre-analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood ................................................. 81 Wendy L.J. Hansen, Cathrien A. Bruggeman, and Petra F.G. Wolffs 5 Detection of Bacterial Contamination in Platelet Concentrates Using Flow Cytometry and Real Time PCR Methods ..................................................... 91 Tanja Vollmer, Knut Kleesiek, and Jens Dreier 6 Multiplex Real-Time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton- Valentine Leukocidin from Clinical Samples ................. 105 Lynne Renwick, Anne Holmes, and Kate Templeton 7 PCR Detection of Haemophilus influenzae from Respiratory Specimens .................................................. 115 Guma M.K. Abdeldaim and Bjorn Herrmann 8 Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-Time PCR ................................. 125 Clare L. Ling and Timothy D. McHugh 9 Utilization of Multiple Real-Time PCR Assays for the Diagnosis of Bordetella spp. in Clinical Specimens ......... 135 Kathleen M. Tatti and Maria Lucia Tondella 10 Detection of Mycoplasma pneumoniae by Real Time PCR ........ 149 Jonas M. Winchell and Stephanie L. Mitchell 11 Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia ........................................ 159 Judith Fillaux and Antoine Berry 12 Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens ............. 171 Wing-Cheong Yam and Kit-Hang Gilman Siu 13 Direct Detection of Mycobacterium ulcerans in Clinical Specimens and Environmental Samples ........................ 201 Caroline J. Lavender and Janet A.M. Fyfe 14 Detection of Bartonella spp. DNA in Clinical Specimens Using an Internally Controlled Real Time PCRAssay .......... 217 Anneke M.C. Bergmans and John W.A. Rossen 15 Simultaneous Direct Identification of Genital Microorganisms in Voided Urine Using Multiplex PCR-Based Reverse Line Blot Assays ................................... 229 Michelle L. McKechnie, Fanrong Kong, and Gwendolyn L. Gilbert 16 Diagnosis of Clostridium difficile Infection Using Real- Time PCR ................................................... 247 Renate Johanna van den Berg, Dennis Bakker, and Ed J. Kuijper 17 Detection of Pathogenic Leptospira spp. Through Real-Time PCR (qPCR) Targeting the LipL32 Gene ....................... 257 Robyn Anne Stoddard 18 Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli Using Loop-Mediated Isothermal Amplification .............................................. 267 Wataru Yamazaki 19 PCR Detection of Helicobacter pylori in Clinical Samples ... 279 Emiko Rimbara, Masanori Sasatsu, and David Y. Graham 20 Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-Time PCR .................... 289 Andrée F. Maheux, Luc Bissonnette, and Michel G. Bergeron 21 Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic ........ 307 Francesca Barletta, Theresa J. Ochoa, and Thomas G. Cleary Index ......................................................... 315