Metastasis research protocols / ed. by M.Dwek, S.A.Brooks, U.Schumacher. - 2nd ed. - New York: Humana Press/Springer, 2012. - xii, 274 p.: ill. - (Methods in molecular biology; 878). - Incl. bibl. ref. - Ind.: p.273-274. - ISBN 978-1-61779-853-5; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 Basic Immunocytochemistry for Light Microscopy ............... 1 Susan A. Brooks 2 Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues ........................................... 31 Susan A. Brooks and Debbie M.S. Hall 3 Histopathological Assessment of Metastasis .................. 51 Derek E. Roskell and Ian D. Buley 4 Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells ..................... 63 Stephan Braun and Klaus Pantel 5 The Polymerase Chain Reaction ............................... 71 Hazel M. Welch 6 Sodium Dodecyl Sulphate-Polyacrylamide Denaturing Gel Electrophoresis and Western Blotting Techniques ............. 89 Christine Blancher and Rob Mc Cormick 7 2-DE-Based Proteomics for the Analysis of Metastasis- Associated Proteins ........................................ 111 Miriam Dwek and Diluka Peiris 8 Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography ................................................. 121 Marta Toth, Anjum Sohail, and Rafael Fridman 9 Determination of Cell-Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer ........................................ 137 Michael R. Dashwood and Marilena Loizidou 10 Fluorescence In Situ Hybridization for Cancer-Related Studies .................................................... 149 Lyndal Kearney and Janet Shipley 11 Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells ................ 175 Julien Saint-Guirons and Björn Ingemarsson 12 Cell Separations by Flow Cytometry ......................... 185 Derek Davies 13 Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cell Cultures .......... 201 Matthias Christyen, Matthias Ballmaier, Ulrich Lehmann, and Hans Kreipe 14 Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours ............................................ 217 Caroline Schreiber, Kirsten Vormbrock and Ulrike Ziebold 15 High-resolution Quantitative Methylation Analysis of microRNA Genes using PyrosequencingTM ...................... 229 Ulrich Lehmann, Cord Albat, and Hans Kreipe 16 RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example .......................... 241 Daniel Wicklein 17 GaIectin-3 Binding and Metastasis .......................... 251 Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz 18 Lectin Array-Based Strategies for Identifying Metastasis- Associated Changes in Glycosylation ........................ 267 Simon Fry, Babak Afrough, Anthony Leathern, and Miriam Dwek Index ......................................................... 273