Preface ......................................................... v
Contributors ................................................... xi
Part I Review
1 Ribosome Display: A Perspective .............................. 3
Andreas Plückthun
Part II Translation Extract Preparation
2 Preparation and Testing of E. coli S30 In Vitro
Transcription Translation Extracts .......................... 31
James F. Zawada
Part III Basic Ribosome Display and Related Selection Methods
3 Eukaryotic Ribosome Display Selection Using Rabbit
Reticulocyte Lysate ......................................... 45
Julie A. Douthwaite
4 Stabilized Ribosome Display for In Vitro Selection .......... 59
Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li,
and Yoshibiro Ito
5 Eukaryotic Ribosome Display with In Situ DNA Recovery ....... 75
Mingyue He, Bryan M. Edwards, Damjana Kastelic, and
Michael J. Taussig
6 mRNA Display Using Covalent Coupling of mRNA to Translated
Proteins .................................................... 87
Kong Wang, Steve W. Gotten, and Rihe Liu
7 SNAP Display: In Vitro Protein Evolution in Microdroplets .. 101
Miriam Kaltenbach and Florian Hollfelder
8 cDNA Display: Rapid Stabilization of mRNA Display .......... 113
Shingo Ueno and Naoto Nemoto
Part IV Applications of Ribosome Display Methods Using
Natural Amino Acids
9 Optimisation of Antibody Affinity by Ribosome Display
Using Error-Prone or Site-Directed Mutagenesis ............. 139
Leeanne Lewis and Chris Lloyd
10 Affinity Maturation of Phage Display Antibody Populations
Using Ribosome Display ..................................... 163
Maria A. Groves and Adrian A. Nickson
11 Evolution of protein Stability Using Ribisome Display ...... 191
Andrew Buchanan
12 Selection of Lead Antibodies from Naive Ribosome
Display Antibody Libraries ................................. 213
Peter Ravn
13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA
Display .................................................... 237
Yuki Mochizuki and Naoto Nemoto
14 Peptide Screening Using PURE Ribosome Display .............. 251
Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang
K. Akbar, and Takuya Ueda
15 Rapid Selection of High-Affinity Binders Using Ribosome
Display .................................................... 261
Birgit Dreier and Andreas Plückthun
16 mRNA Display-Based Selections Using Synthetic Peptide
and Natural Protein Libraries .............................. 287
Steve W. Gotten, Jianwei Zou, Rong Wang, Bao-cheng Huang,
and Rihe Liu
17 Identification of Candidate Vaccine Genes Using Ribosome
Display .................................................... 299
Liancheng Lei
18 Ribosome Display for the Selection of Sac7d Scaffolds ...... 315
Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-
Laurance, Stéphane Colinet, and Frédéric Pecorari
Part V Incorporation of Non-Natural Amino Acids for
Selection by Ribosome Display and Related Methods
19 Charging of tRNAs Using Ribozymes and Selection of
Cyclic Peptides Containing Thioethers ...................... 335
Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki
Suga
20 Update on Pure Translation Display with Unnatural Amino
Acid Incorporation ......................................... 349
R. Edward Watts and Anthony C. Forster
21 In Vitro Selection of Unnatural Cyclic Peptide Libraries
via mRNA Display ........................................... 367
Zhong Ma and Matthew C.T. Hartman
Part VI Case Studies
22 Optimization of CAT-354, a Therapeutic Antibody Directed
Against Interleukin-13, Using Ribosome Display ............. 393
George Thom and Ralph Minter
23 Affinity Maturation and Functional Dissection of
a Humanised Anti-RAGE Monoclonal Antibody by Ribosome
Display .................................................... 403
Simon E. Hufton
Index ......................................................... 423
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