Preface ....................................................... vii
Contributors ................................................. xiii
1 The Tomato Leaf as a Model System for Organogenesis .......... 1
Yogev Burko and Naomi Ori
2 Usefulness of Physcomitrella patens for Studying Plant
Organogenesis ............................................... 21
Sandrine Bonhomme, Fabien Nogué, Catherine Rameau, and
Didier G. Schaefer
3 The Dicot Root as a Model System for Studying
Organogenesis ............................................... 45
Julien Lavenus, Mikaël Lucas, Laurent Laplaze, and Soazig
Guyomarc'h
4 Genetic Control of Root Organogenesis in Cereals ............ 69
Caroline Mar con, Anja Paschold, and Frank Hochholdinger
5 Gene Expression Analysis of Aquatic Angiosperms
Podostemaceae to Gain Insight into the Evolution of Their
Enigmatic Morphology ........................................ 83
Satoshi Koi and Natsu Katayama
6 Brown Algae as a Model for Plant Organogenesis .............. 97
Kenny A. Bogaert, Alok Arun, Susana M. Coelho, and Olivier
De Clerck
7 Microscopic Analysis of Ovule Development in Arabidopsis
thaliana ................................................... 127
Balaji Enugutti, Maxi Oelschner, and Kay Schneitz
8 Imaging of Phenotypes, Gene Expression, and Protein
Localization During Embryonic Root Formation in
Arabidopsis ................................................ 137
Cristina Llavata-Peris, Annemarie Lokerse, Barbara
Möller, Bert De Rybel, and Dolf Weijers
9 Inducible System for Lateral Roots in Arabidopsis
thaliana and Maize ......................................... 149
Leen Jansen, Boris Parizot, and Tom Beeckman
10 Adventitious Root Induction in Arabidopsis thaliana as
a Model for In Vitro Root Organogenesis .................... 159
Inge Verstraeten, Tom Beeckman, and Danny Geelen
11 High-Throughput, Noninvasive Imaging of Root Systems ....... 177
Anjali S. Iyer-Pascuzzi, Paul R. Zurek, and Philip
N. Benfey
12 Small-Molecule Screens to Study Lateral Root Development ... 189
Dominique Audenaert, Bert De Rybel, Long Nguyen, and Tom
Beeckman
13 Cell Lineage Analyses in Living Tissues .................... 197
John Runions and Smita Kurup
14 Protein Immunolocalization in Maize Tissues ................ 207
Cristian Forestan, Nicola Carraro, and Serena Varotto
15 Auxin Immunolocalization in Plant Tissues .................. 223
Cristian Forestan and Serena Varotto
16 Gene Expression Analysis of Shoot Apical Meristem Cell
Types ...................................................... 235
Ram Kishor Yadav, Stephen Snipes, Thomas Girke, and
G. Venugopala Reddy
17 Kinematic Analysis of Cell Division in Leaves of Mono-
and Dicotyledonous Species: A Basis for Understanding
Growth and Developing Refined Molecular Sampling
Strategies ................................................. 247
Hilde Nelissen, Bart Rymen, Frederik Coppens, Stijn
Dhondt, Fabio Fiorani, and Gerrit T.S. Beemster
18 Regeneration in Arabidopsis Tissue Culture ................. 265
Kaoru Sugimoto and Elliot M. Meyerowitz
19 Isolation and Analysis of mRNAs from Specific Cell Types
of Plants by Ribosome Immunopurification ................... 277
Angelika Mustroph, M. Eugenia Zanetti, Thomas Girke, and
Julia Bailey-Serres
20 Analyzing Small and Long RNAs in Plant Development Using
Non-radioactive In Situ Hybridization ...................... 303
Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier,
Christine Lelandais-Brière, and Martin Crespi
21 Analyzing Protein Distribution in Plant Tissues Using
"Whole-Mount" Immunolocalization ........................... 317
Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier,
Christine Lelandais-Brière, and Martin Crespi
22 Culture Methods and Mutant Generation in the Filamentous
Brown Algae Ectocarpus siliculosus ......................... 323
Aude Le Bail and Benedicte Charrier
23 Building Simulation Models of Developing Plant Organs
Using VirtualLeaf .......................................... 333
Roeland M.H. Merks and Michael A. Guravage
Index ......................................................... 353
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