Phytoplasma: methods and protocols / ed. by M.Dickinson, J.Hodgetts. - New York: Humana Press, 2013. - xiii, 421 p.: ill. - (Methods in molecular biology; 938) (Springer protocols). - Incl. bibl. ref. - Ind.: p.417-421. - ISBN 978-1-62703-088-5; ISSN 1064-3745  Место хранения:   05 | Сибирский институт физиологии и биохимии растений СО РАН | Иркутск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi 1 The Phytoplasmas: An Introduction ............................ 1 Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts 2 Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus ...................................................... 15 Jennifer Hodgetts, David Crossley, and Matt Dickinson 3 Micropropagation and Maintenance of Phytoplasmas in Tissue Culture ..................................................... 33 Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo 4 Dodder Transmission of Phytoplasmas ......................... 41 Jaroslava Pribylovd and Josef Spak 5 Insect Maintenance and Transmission ......................... 47 Heather Kingdom 6 Capturing Insect Vectors of Phytoplasmas .................... 61 Phyllis Weintraub and Jürgen Gross 7 Insect Vector Transmission Assays ........................... 73 Domenico Bosco and Rosemarie Tedeschi 8 Molecular Identification of Phytoplasma Vector Species ...... 87 Sabrina Bertin and Domenico Bosco 9 Dienes'Staining and Light Microscopy for Phytoplasma Visualization .............................................. 109 Rita Musetti 10 DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas ............................................... 115 Nancy M. Andrade and Nolberto L. Arismendi 11 Visualization of Phytoplasmas Using Electron Microscopy .... 123 B. Jean Devonshire 12 Automated DNA Extraction for Large Numbers of Plant Samples .................................................... 139 Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia 13 DNA Extraction from Arborescent Monocots and How to Deal with Other Challenging Hosts ............................... 147 Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick 14 Nested PCR and RPLP Analysis Based on the 16S rRNA Gene .... 159 Bojan Duduk, Samanta Paltrinieri, Ing-Ming Lee, and Assunta Bertaccini 15 PCR and RFLP Analyses Based on the Ribosomal Protein Operon ..................................................... 173 Marta Martini and Ing-Ming Lee 16 Tuf and secY VCR Amplification and Genotyping of Phytoplasmas ............................................... 189 Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová, and Milan Navrátil 17 PCR Analysis of Phytoplasmas Based on the secA Gene ........ 205 Matt Dickinson and Jennifer Hodgetts 18 Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains ........................ 217 Martina Šеruga Musić and Dijana Škorić 19 Microarrays for Universal Detection and Identification of Phytoplasmas ............................................... 223 Mogens Nicolaisen, Henrietta Nyskjold, and Assunta Bertaccini 20 T-RFLP for Detection and Identification of Phytoplasmas in Plants .................................................. 233 Jennifer Hodgetts and Matt Dickinson 21 Real-Time PCR for Universal Phytoplasma Detection and Quantification ............................................. 245 Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen 22 A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA ................................................. 253 Nataša Mehle, Nina Prezelj, Matjaž Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia 23 Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group ............ 269 Nataša Mehle, Petra Nikolić, Kristina Gruden, Maja Ravnikar, and Marina Dermastia 24 Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions ............................ 283 Paolo Margaria and Sabrina Palmano 25 In-Field Diagnostics Using Loop-Mediated Isothermal Amplification .............................................. 291 Jenny Tomlinson 26 DNA Bar-Coding for Phytoplasma Identification .............. 301 Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, and Mogens Nicolaisen 27 Phylogenetic Analyses of Phytoplasmas Based on Whole- Genome Comparison .......................................... 319 Hiromi Nishida 28 The i'PhyClassifier, an Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment ........ 329 Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, and Robert E. Davis 29 Phytoplasma Proteomic Analysis ............................. 339 Xianling Ji and Yingping Gai 30 Preparation of Phytoplasma Membrane Recombinant Proteins ... 351 Luciana Galetto, Majid Siampour, and Cristina Marzachi 31 Phytoplasma Plasmid DNA Extraction ......................... 371 Mark T. Andersen and Lia W. Liefting 32 Cesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA .................................. 381 Lucy T.T. Tran-Nguyen and Bernd Schneider 33 Pulsed-Field Gel Electrophoresis for Isolation of Full- Length Phytoplasma Chromosomes from Plants ................. 395 Carmine Marcone 34 Mapping the Phytoplasma Chromosome ......................... 405 Sylvie Malembic-Maher and Patricia Carle Index ......................................................... 417