Preface ......................................................... v
Contributors ................................................... xi
1 Purification of Human NK Cell Developmental Intermediates
from Lymph Nodes and Tonsils ................................. 1
Aharon G. Freud and Michael A. Caligiuri
2 In Vitro Development of Human Killer-Immunoglobulin
Receptor-Positive NK Cells .................................. 15
Frank Cichocki and Jeffrey S. Miller
3 Subset Analysis of Human and Mouse Mature NK Cells .......... 27
Yoshihiro Hayakawa, Daniel M. Andrews, and Mark J. Smyth
4 Assessing Licensing of NK Cells ............................. 39
A. Helena Jonsson and Wayne M. Yokoyama
5 Use of Stem Cell Radiation Chimeras to Analyze How Domains
of Specific Proteins Impact on Murine NK Cell Development
In Vivo ..................................................... 51
Rebecca H. Lian and Vinay Kumar
6 Use of Transfected Drosophila S2 Cells to Study NK Cell
Activation .................................................. 67
Michael E. March, Catharina C. Gross, and Eric O. Long
7 Natural Killer Cell Conjugate Assay Using Two-Color Flow
Cytometry ................................................... 89
Deborah N. Burshtyn and Chelsea Davidson
8 Studying NK Cell/Dendritic Cell Interactions ................ 97
Mathias Lucas, Cedric Vonarbourg, Peter Aichele, and
Andreas Diefenbach
9 Analysis of the NK Cell Immunological Synapse .............. 127
Keri B. Sanborn, Gregory D. Rak, Ashley N. Mentlik,
Pinaki P. Banerjee, and Jordan S. Orange
10 Measuring Intracellular Calcium Signaling in Murine NK
Cells by Flow Cytometry .................................... 149
Alexander W. MacFarlane IV, James F. Oesterling, and
Kerry S. Campbell
11 Intracellular Staining for Analysis of the Expression and
Phosphorylation of Signal Transducers and Activators of
Transcription (STATs) in NK Cells .......................... 159
Takuya Miyagi, Seung-Hwan Lee, and Christine A. Biron
12 A Model System for Studying NK Cell Receptor Signaling ..... 177
Lukasz K. Chlewicki and Vinay Kumar
13 Expression of cDNAs in Human Natural Killer Cell Lines
by Retroviral Transduction ................................. 199
S.M. Shahjahan Miah and Kerry S. Campbell
14 Lentiviral Gene Transduction in Human and Mouse NK Cell
Lines ...................................................... 209
Ram Savan, Tim Chan, and Howard A. Young
15 Introduction of shRNAs into Human NKTike Cell Lines with
Retrovirus ................................................. 223
Amanda K. Purdy and Kerry S. Campbell
16 Introduction of shRNAs into Primary NK Cells with
Lentivirus ................................................. 233
Sam K.P. Kung
17 Methods to Identify and Characterize Different NK Cell
Receptors and Their Ligands ................................ 249
Dikla Lankry, Roi Gazit, and Ofer Mandelboim
18 Generating NK Cell Receptor-Fc Chimera Proteins from ....... 293
T Cells and Considerations of Appropriate Glycosylation .... 275
Alon Zilka, Michal Mendelson, Benyamin Rosental, Oren
Hershkovitz, and Angel Porgador
19 Identification of NK Cell Receptor Ligands Using
a Signaling Reporter System ................................ 285
Yoshie-Matsubayashi Iizuka, Nikunj V. Somia, and Koho
Iizuka
20 Determining Ligand Specificity of Ly49 Receptors ........... 299
Kerry J. Lavender and Kevin P. Kane
21 Probing the Interactions of NK Cell Receptors with Ligand
Expressed in trans and cis ................................. 313
Jonathan Back, Leonardo Scarpellino, and Werner Held
22 A Simple Method to Measure NK Cell Cytotoxicity In Vivo .... 325
Aurore Saudemont, Shannon Burke, and Francesco Colucci
23 Functional Analysis of Human NK Cells by Flow Cytometry .... 335
Yenan T. Bryceson, Cyril Fauriat,Joao M. Nunes, Stephanie
M. Wood, Niklas K. Björkstrom, Eric O. Long, and
Hans-Gustaf Ljunggren
24 Analysis of the KIR Repertoire in Human NK Cells by Flow
Cytometry .................................................. 353
Niklas K. Björkstrom, Cyril Fauriat, Yenan T. Bryceson,
Johan K. Sandberg, Hans-Gustaf Ljunggren, and Karl-Johan
Malmberg
25 KIR Genotyping by Multiplex PCR-SSP ........................ 365
Smita Kulkarni, Maureen P. Martin, and Mary Carrington
26 Identification and Analysis of Novel Transcripts and
Promoters in the Human Killer Cell Immunoglobulin-like
Receptor (KIR) Genes ....................................... 377
Hongchuan Li, Paul W. Wright, and Stephen K. Anderson
27 Use of Inbred Mouse Strains to Map Recognition Receptors
of MCMV Infected Cells in the NK Cell Gene Locus ........... 393
Nassima Fodil-Cornu, Michal Pyzik, and Silvia M. Vidal
28 Studying NK Cell Responses to Ectromelia Virus Infections
in Mice .................................................... 411
Min Fang and Luis Sigal
29 Activation of Human NK Cells by Malaria-Infected Red
Blood Cells ................................................ 429
Amir Horowitz and Eleanor M. Riley
30 Natural Killer Cells in Human Pregnancy .................... 447
Victoria Male, Anita Trundley, Lucy Gardner, Jacquie
Northfield, Chiwen Chang, Richard Apps, and Ashley
Moffett
31 Analysis of Uterine Natural Killer Cells in Mice ........... 465
B. Anne Croy, Jianhong Zhang, Chandrakant Tayade,
Francesco Colucci, Hakim Tadi, and Aureo T. Yamada
32 Isolation of NK Cells and NK-Like Cells from the
Intestinal Lamina Propria .................................. 505
Stephanie L. Sanos and Andreas Diefenbach
Appendix ...................................................... 519
Index ......................................................... 545
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