Preface ......................................................... V
Contributors ................................................... XI
1 Immobilization of Enzymes as the 21 st Century Begins:
An Already Solved Problem or Still an Exciting Challenge?
Jose M. Guisan ............................................... 1
2 Immobilization of Enzymes: A Literature Survey
Beatriz M. Brena and Francisco Batista-Viera ................ 15
3 Cross-Linked Enzyme Aggregates
Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen .... 31
4 Immobilization-Stabilization of Enzymes by Multipoint
Covalent Attachment on Supports Activated With Epoxy
Groups
Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente,
Benevides С.C. Pessela, Valeria Grazu, Jose M. Guisan,
and Roberto Fernandez-Lafuente .............................. 47
5 Glutaraldehyde in Protein Immobilization: A Versatile
Reagent
Lorena Betancor, Fernando López-Gallego, Noelia
Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto
Fernandez-Lafuente, and Jose M. Guisan ..................... 57
6 Practical Protocols for Lipase Immobilization Via Sol-Gel
Techniques
Manfred T. Reetz ........................................... 65
7 Encapsulation of Enzymes Using Polymers and Sol-Gel
Techniques
Monica Campus and Jean-Louis Marty ......................... 77
8 Design of Smart Biocatalysts: Immobilization of Enzymes
on Smart Polymers
Ipsita Roy and Munishwar N. Gupta ........................... 87
9 Affinity Immobilization of Tagged Enzymes
Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty ....... 97
10 Bioaffinity Immobilization
Ipsita Roy and Munishwar N. Gupta .......................... 107
11 One-Step Purification, Immobilization, and Stabilization
of Poly-Histidine-Tagged Enzymes Using Metal Chelate-
Epoxy Supports
Cesar Mateo, Benevides С.C. Pessela, Valeria Grazu,
Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan,
and Roberto Fernandez-Lafuente ............................. 117
12 Stabilization of Multimeric Enzymes Via Immobilization
and Further Cross-Linking With Aldehyde-Dextran
Cesar Mateo, Benevides С.C. Pessela, Manuel Fuentes,
Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo,
Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente,
and Jose M. Guisan ......................................... 129
13 Purification, Immobilization, Hyperactivation, and
Stabilization of Lipases by Selective Adsorption on
Hydrophobic Supports
Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo,
Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-
Lafuente, and Jose M. Guisan ............................... 143
14 Immobilization and Stabilization of Proteins by
Multipoint Covalent Attachment on Novel Amino-Epoxy-
Sepabeads®
Cesar Mateo, Benevides С.C. Pessela, Valeria Grazu,
Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes,
Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor,
Gloria Fernández-Lorente, Claudia Ortiz, Olga Abian,
Jose M. Guisan, and Roberto Fernandez-Lafuente ............ 153
15 Improved Stabilization of Chemically Aminated Enzymes
Via Multipoint Covalent Attachment on Glyoxyl Supports
Tamara Montes, Fernando López-Gallego, Manuel Fuentes,
Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose
M. Guisan, and Roberto Fernandez-Lafuente .................. 163
16 Stabilization of New Imprint Property of Glucose Oxidase
in Pure Aqueous Medium by Cross-Linked-lmprinting
Approach
Alankar Vaidya and Lutz Fischer ............................ 775
17 Reversible Covalent Immobilization of Enzymes Via Their
Thiol Groups
Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta .. 185
18 Very Strong But Reversible Immobilization of Enzymes on
Supports Coated With Ionic Polymers
Cesar Mateo, Benevides С.C. Pessela, Manuel Fuentes,
Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego,
Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan,
and Roberto Fernandez-Lafuente ............................. 205
19 Immobilization of Enzymes on Magnetic Particles
Martina Konerackd, Peter Kopčanský, Milan Timko, Chenyl
Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan,
and Anil de Sequeira ....................................... 217
20 Immobilization of Proteins on Gold Surfaces
José M. Abad, Marcos Pita, and Victor M. Fernández ......... 229
21 Immobilization of Enzymes on Electrodes Gilvanda Silva
Nunes and Jean-Louis Marty ................................. 239
22 Immobilization of Enzymes for Use in Organic Media
Patrick Adlercreutz ........................................
23 Immobilization of Enzymes for Use in Ionic Liquids
Pedro Lozano, Teresa de Diego, and José L. Iborra .......... 257
24 Immobilization of Enzymes for Use in Supercritical
Fluids
Pedro Lozano, Teresa de Diego, and José L. Iborra .......... 269
25 Immobilized Enzymes for Biomedical Applications
Amaia Esquisabel, Rosa María Hernández, Alicia Rodríguez
Gascón, and José Luis Pedraz ............................... 283
26 Characterization of Immobilized Enzymes by
Microcalorimetry Ezio Battistel and Giovanni Rialdi ........ 295
27 Use of Immobilized Biocatalysts in Fluidized Bed Format
Ipsita Roy and Munishwar N. Gupta .......................... 311
28 Taylor-Couette Vortex Flow in Enzymatic Reactors
Roberto Campos Giordano and Raquel de Lima Camargo
Giordano ................................................... 321
29 A Novel Immobilization Method for Entrapment: LentiKats®
Marc Schlieker and Klaus-Dieter Vorlop ..................... 333
30 Encapsulation of Cells in Alginate Gels
Gorka Orive, Rosa María Hernández, Alicia Rodríguez
Gascón, and José Luis Pedraz ............................... 345
31 Immobilization of Cells on Polyurethane Foam
Ignacio de Ory, Gema Cabrera, Martin Ramirez, and Ana
Blandino ................................................... 357
32 Immobilization of Cells With Transition Metal
Pedro Fernandes ............................................ 367
33 Immobilization of Microalgae
Nirupama Mallick ........................................... 373
34 Bioluminescence in Immobilized Cells for Biomass
Detection and Biosensor Applications
Marián Navratil, Juraj Svitel, Peter Gemeiner .............. 393
35 A Proteomic Approach to Biofilm Cell Physiology
Laurent Coquet, Sébastien Vilain, Pascal Cosette, Thierry
Jouenne, and Guy-Alain Junter .............................. 403
36 Encapsulation of Bacteria for Biodegradation
of Gasoline Hydrocarbons
Peyman Moslemy, Serge R. Guiot, and Ronald J. Neufeld ...... 415
37 Biomedical Applications of Immobilized Cells
Gorka Orive, Rosa María Hernández, Alicia Rodríguez
Gascón, and José Luis Pedraz ............................... 427
Index ......................................................... 439
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