Preface ......................................................... v
Contributors ................................................... ix
1 Cell Senescence as Both a Dynamic and a Static Phenotype ..... 1
Andrew R.J. Young, Masako Narita, and Masashi Narita
2 Senescence Regulation by mTOR ............................... 15
Vjekoslav Dulic
3 Senescence Regulation by the p53 Protein Family ............. 37
Yingjuan Qian and Xinbin Chen
4 Markers of Cellular Senescence .............................. 63
Amancio Carnero
5 Biomarkers of Cell Senescence Assessed by Imaging
Cytometry ................................................... 83
Hong Zhao and Zbigniew Darzynkiewicz
6 Cytofluorometric Assessment of Cell Cycle Progression ....... 93
Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier
Metivier, Maria Castedo, and Guido Kroemer
7 Quantification of Cell Cycle-Arresting Proteins ............ 121
Oliver Kepp, Isabelle Martins, Laurie Menger, Mickael
Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo
Galluzzi, and Guido Kroemer
8 Colorimetric Detection of Senescence-Associated
(3 Galactosidase ........................................... 143
Koji Itahana, Yoko Itahana, and Goberdhan P. Dimri
9 Chemiluminescent Detection of Senescence-Associated p
Galactosidase .............................................. 157
Vinicius Bassaneze, Ayumi Aurea Miyakawa, and José
Eduardo Krieger
10 Detection of the Senescence-Associated Secretory
Phenotype (SASP) ........................................... 165
Francis Rodier
11 Unbiased Characterization of the Senescence-Associated
Secretome Using SILAC-Based Quantitative Proteomics ........ 175
Juan Carlos Acosta, Ambrosius P. Snijders, and Jesús Gil
12 Detection of Senescence-Associated Heterochromatin Foci
(SAHF) ..................................................... 185
Katherine M. Aird and Rugang Zhang
13 Monitoring DNA Damage During Cell Senescence ............... 197
Glyn Nelson and Thomas von Zglinicki
14 Assessment and Quantification of Telomerase Enzyme
Activity ................................................... 215
Michelle F. Maritz, Laura A. Richards, and Karen
L. MacKenzie
15 Methods for the Assessment of Telomere Status .............. 233
Asako J. Nakamura
16 Detection of Nuclear Envelope Alterations in Senescence .... 243
Clea Bárcena, Fernando G. Osorio, and Jose Maria Pérez
Freije
17 Measuring Reactive Oxygen Species in Senescent Cells ....... 253
João F. Passes, Satomi Miwa, and Thomas von Zglinicki
18 Quantification of Protein Carbonylation .................... 265
Nancy B. Wehr and Rodney L. Levine
19 Assays for the Measurement of Lipid Peroxidation ........... 283
Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic,
Suzana Borovic Sunjic, and Neven Zarkovic
20 Raman Spectroscopy for the Detection of AGEs/ALEs .......... 297
J. Renwick Beattie, John J. McGarvey, and Alan W. Stitt
21 Monitoring Oncogenic B-RAF-Induced Senescence in
Melanocytes ................................................ 313
Sieu L. Tran and Helen Rizos
22 Methods to Investigate the Role of SIRT1 in Endothelial
Senescence ................................................. 327
Bo Bai and Yu Wang
23 Monitoring Nutrient Signaling Through the Longevity
Protein p66SHCI ............................................ 341
Sofia Chiatamone Ranieri and Giovambattista Pani
24 Profiling the Metabolic Signature of Senescence ............ 355
Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand
M. Leroi, and Jacob G. Bundy
25 Genome-Wide RNAi Screening to Identify Regulators of
Oncogene-Induced Cellular Senescence ....................... 373
Narendra Wajapeyee, Sara K. Deibler, and Michael
R. Green
26 An Integrated Approach for Monitoring Cell Senescence ...... 383
Tatiana V. Pospelova, Zhanna V. Chitikova, and Valery
A. Pospelov
27 Robust Multiparametric Assessment of Cellular Senescence ... 409
Clara Correia-Melo, Diana Jurk, and João F. Passes
28 Assessing Chronological Aging in Bacteria .................. 421
Stavros Gonidakis and Valter D. Longo
29 Assessing Organismal Aging in the Filamentous Fungus
Podospora anserina ......................................... 439
Heinz D. Osiewacz, Andrea Hamann, and Sandra Zintel
30 Assessing Chronological Aging in Saccharomyces cerevisiae .. 463
Jia Нu, Min Wei, Mario G. Mirisola, and Valter D. Longo
31 Assessing Aging and Senescent Decline in Caenorhabditis
elegans: Cohort Survival Analysis .......................... 473
Eirini Lionaki and Nektarios Tavernarakis
32 High-Throughput and Longitudinal Analysis of Aging and
Senescent Decline in Caenorhabditis elegans ................ 485
Eirini Lionaki and Nektarios Tavernarakis
33 Assessing Senescence in Drosophila Using Video Tracking .... 501
Reza Ardekani, Simon Tavaré, and John Tower
34 Assessing Vascular Senescence in Zebrafish ................. 517
Sandra Donnini, Antonio Giachetti, and Marina Ziche
Index ......................................................... 533
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