Histology protocols / ed. by T.D.Hewitson, I.A.Darby. - New York: Humana Press, 2010. - x, 229 p.: ill. - (Methods in molecular biology; 611) (Springer protocols). - Incl. bibl. ref. and index. - ISBN 978-1-60327-344-2; ISSN 1064-3745  Место хранения:   07 | Центральная библиотека Красноярского научного центра СО РАН | Красноярск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix Part I: Tissue Processing 1 Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy ........ 3 Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker 2 An Optimized RNA Extraction Method from Archival Formalin- Fixed Paraffin-Embedded Tissue .............................. 19 Joon-Yong Chung and Stephen M. Hewitt 3 Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus ..... 29 Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly Part II: Staining Techniques 4 Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells ........... 43 Josiane Smith-Clerc and Boris Hinz 5 Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems .................. 59 Vincent Sarrazy and Alexis Desmoulière 6 Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections ..... 73 Emilio Badoer 7 High-Pressure Freezing, Chemical Fixation and Freeze- Substitution for Immuno-electron Microscopy ................. 87 Christian Mühlfeld 8 Lectin Histochemistry for Light and Electron Microscopy .... 103 Su Ее Wong, Catherine E. Winbanks, Chrishan S. Samuel, and Tim D. Hewitson 9 Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain ...................... 115 Raphael Pinaud and Jin K. Jeong 10 Special Stains for Extracellular Matrix .................... 131 Andrea Monte-Alto-Costa and Luís Cristóvão Porto 11 Active Staining of Mouse Embryos for Magnetic Resonance Microscopy ................................................. 141 Alexandra Petiet and G. Allan Johnson 12 Immunohistochemical Detection of Tumour Hypoxia ............ 151 Richard J. Young and Andreas Möller 13 In Situ Localization of Apoptosis Using TUNEL .............. 161 Tim D. Hewitson and Ian A. Darby Part III: Imaging Techniques 14 Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord .............................. 173 Martin Stebbing, Simon Potocnik, Pinglu Yе, and Emilio Badoer 15 High-Resolution Confocal Imaging in Tissue ................. 183 Verena C. Wimmer and Andreas Möller 16 Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography ........ 193 Jörg Piper 17 Image Analysis and Quantitative Morphology ................. 211 Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes- Santos, and Marcia Barbosa Aguila Subject Index ................................................. 227