Transgenic mouse methods and protocols / ed. by M.H.Hofker, J.M. van Deursen. - 2nd ed. - New York: Humana Press, 2011. - xi, 352 p.: ill. - (Methods in molecular biology; vol.693) (Springer protocols). - Incl. bibl. ref. - Ind.: p.345-352. - ISBN 978-1-60761-973-4; ISSN 1064-3745  Место хранения:   021 | Институт цитологии и генетики CO РАН | Новосибирск | Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 Introduction: Strategies for Developing Genetically Modified Mice ................................................ 1 Marten H. Hofker 2 Genetic Modification of the Mouse: General Technology - Pronuclear and Blastocyst Injection ......................... 11 Jan Willem Voncken 3 Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos .............. 37 Jérôme Artus and Anna-Katerina Hadjantonakis 4 Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization ............................................... 57 Naomi Nakagata 5 Autopsy and Histological Analysis of the Transgenic Mouse ... 75 Marion J.J. Gijbels and Menno P.J. de Winther 6 Transgene Design ............................................ 89 Bart van de Sluis and Jan Willem Voncken 7 Inducible Transgenic Mouse Models .......................... 103 Thomas L. Saunders 8 Lentiviral Transgenesis .................................... 117 Terunaga Nakagawa and Casper C. Hoogenraad 9 Transgenesis in Mouse Embryonic Stem Cells ................. 143 Janine van Ree, Wei Zhou, Ming Li, and Jan M. van Deursen 10 Engineering ВАС Reporter Gene Constructs for Mouse Transgenesis ............................................... 163 Yu Fu and Peter Maye 11 Targeting Vector Construction Through Recombineering ....... 181 Liviu A. Malureanu 12 Generating Conditional Knockout Mice ....................... 205 Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers, and Ralf Kühn 13 Hypomorphic Mice ........................................... 233 Darren J. Baker 14 MICER Targeting Vectors for Manipulating the Mouse Genome .. 245 Chunhong Liu, Paul F. Szurek, and Y. Eugene Yu 15 Knock-In Approaches ........................................ 257 Anton J.M. Roebroek, Philip L.S.M. Gordts, and Sara Reekmans 16 Generation of a Series of Knock-In Alleles Using RMCE in ES Cells ................................................... 277 Anton J.M. Roebroek, Philip L.S.M. Gordts, and Sara Reekmans 17 Selection of Targeted Mutants from a Library of Randomly Mutagenized ES Cells ....................................... 283 Kyoji Horie, George Gaitanaris, and Alexander Gragerov 18 Generation of Genetically Modified Rodents Using Random ENU Mutagenesis ............................................ 295 Ruben van Boxtel and Edwin Cuppen 19 Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells ..................................... 309 Menno P.J. de Winther and Peter Heeringa 20 Adenovirus-Mediated Gene Transfer .......................... 321 Ко Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux, and Jurre Hageman Index ......................................................... 345