Preface ....................................................... vii
Contributors ................................................. xiii
Part I General Aspects
1 Using Folding Promoting Agents in Recombinant Protein
Production: A Review ......................................... 3
Beatrix Fahnert
2 Routine Identity Confirmation of Recombinant Proteins
by MALDI-TOF Mass Spectrometry .............................. 37
Brett J. Savary and Prasanna Vasu
3 A Matter of Packaging: Influence of Nucleosome Positioning
on Heterologous Gene Expression ............................. 51
Maria de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano,
and Sebastián Chávez
4 Tools of the Trade: Developing Antibody-Based Detection
Capabilities for Recombinant Proteins ....................... 65
Maureen C. Dolan, Giuliana Medrano, Anthony McMickle, and
Carole L. Cramer
Part II Prokaryotes
5 Heat-Shock Protein Fusion Vectors for Improved Expression
of Soluble Recombinant Proteins in Escherichia coli ........ 109
Christos A. Kyratsous and Christos A. Panagiotidis
6 The Use of a Flagellar Export Signal for the Secretion
of Recombinant Proteins in Salmonella ...................... 131
Ferenc Vonderviszt, Ráchel Sajó, József Dobó, and Péter
Závodszky
7 Optimization of Purification Protocols Based on the
Step-by-Step Monitoring of the Protein Aggregates in
Soluble Fractions .......................................... 145
Ario de Marco
8 Heterologous Protein Expression by Lactococcus lactis ...... 155
Julio Villatoro-Hernández, Oscar P. Kuipers, Odila
Saucedo-Cárdenas, and Roberto Montes-de-Oca-Luna
9 An Extended Suite of Genetic Tools for Use in Bacteria
of the Halomonadaceae: An Overview ......................... 167
Montserrat Argandoña, Carmen Vargas, Mercedes
Reina-Bueno, Javier Rodríguez-Moya, Manuel Salvador, and
Joaquín J. Nieto
10 Regulated Recombinant Protein Production in the Antarctic
Bacterium Pseudoalteromonas haloplanktis TAC125 ............ 203
Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia
Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino,
Gennaro Marino, and Angela Duilio
11 A Novel Strategy for the Construction of Genomic Mutants
of the Antarctic Bacterium Pseudoalteromonas haloplanktis
TAC125 ..................................................... 219
Maria Giuliani, Ermenegilda Parrilli, Cinzia Pezzella,
Valentina Rippa, Angela Duilio, Gennaro Marino, and Maria
Luisa Tutino
12 A New Bacterial Co-expression System for Over-expressing
Soluble Protein and Validating Protein-Protein
Interaction ................................................ 235
Jumei Zeng and Zheng-Guo He
13 Heterologous High-Level Gene Expression in the
Photosynthetic Bacterium Rhodobacter capsulatus ............ 251
Nadine Katzke, René Bergmann, Karl-Erich Jaeger, and
Thomas Drepper
14 Plasmid DNA Production for Therapeutic Applications ........ 271
Alvaro R. Lara and Octavio T. Ramírez
Part III Lower Eukaryotes
15 Recombinant Protein Production in the Eukaryotic
Protozoan Parasite Leishmania tarentolae: A Review ......... 307
Tomoaki Niimi
16 Expression of Multisubunit Proteins in Leishmania
tarentolae ................................................. 317
Marisa Sugino and Tomoaki Niimi
Part IV Fungi
17 Recombinant Protein Production in Yeasts ................... 329
Diethard Mattanovich, Paola Branduardi, Laura Dato,
Brigitte Gasser, Michael Saner, and Danilo Porro
18 Yeasts as a Tool for Heterologous Gene Expression .......... 359
Raja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia
Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak
Borchani-Chabchoub, and All Gargouri
19 The Cre/Lox System: A Practical Tool to Efficiently
Eliminate Selectable Markers in Fungal Endophytes .......... 371
Simona Florea, Caroline Machado, Kalina Andreeva, and
Christopher L. Schardl
20 Aptamer-Regulated Expression of Essential Genes in Yeast ... 381
Beatrix Suess, Karl-Dieter Entian, Peter Kötter, and
Julia E. Weigand
21 Cloning and Expression of Hemicellulases from Aspergillus
nidulans in Pichia pastoris ................................ 393
Prasanna Vasu, Stefan Bauer, and Brett J. Savary
22 A Thiamine-Regulatable Epitope-Tagged Protein Expression
System in Fission Yeast .................................... 417
Tiina Tamm
23 Heterologous Gene Expression by Chromosomal Integration
in Fission Yeast ........................................... 433
Akihisa Matsuyama and Minoru Yoshida
24 Genetic Engineering of Industrial Strains of
Saccharomyces cerevisiae ................................... 451
Sylvie Le Borgne
Part V Plants and Plant Cells
25 Recombinant Protein Production in Plants: Challenges and
Solutions .................................................. 469
Elizabeth E. Hood and Deborah V. Vicuna Requesens
26 A Novel Plant Cell Bioproduction Platform for High-Yield
Secretion of Recombinant Proteins .......................... 483
Jianfeng Xu and Marcia J. Kieliszewski
27 Super-promoter:TEV, a Powerful Gene Expression System
for Tobacco Hairy Roots .................................... 501
Luis Ñopo, Bonnie J. Woffenden, Deborah G. Reed, Scott
Buswell, Chenming Zhang, and Fabricio Medina-Bolivar
28 Bioseparation of Recombinant Proteins from Plant Extract
with Hydrophobin Fusion Technology ......................... 527
Jussi J. Joensuu, Andrew J. Conley, Mark us B. binder,
and Rima Menassa
29 Quality Assessment of Recombinant Proteins Produced in
Plants ..................................................... 535
Giuliana Medrano, Maureen C. Dolan, Jose Condori, David
N. Radin, and Carole L. Cramer
30 Cell-Free Protein Synthesis as a Promising Expression
System for Recombinant Proteins ............................ 565
Xumeng Ge and Jianfeng Xu
Part VI Animals and Animal Cells
31 The Use of Bacterial Artificial Chromosomes for
Recombinant Protein Production in Mammalian Cell Lines ..... 581
Leander Blaas, Monica Musteanu, Beatrice Grabner, Robert
Eferl, Anton Bauer, and Emilio Casanova
32 Engineering the Chaperone Network of CHO Cells for
Optimal Recombinant Protein Production and Authenticity .... 595
Lyne Jossé, C. Mark Smales, and Mick F. Tuite
33 High-Throughput Baculovirus Expression in Insect Cells ..... 609
Richard B. Hitchman, Robert D. Possee, and Linda A. King
34 Recombinant Protein Expression in Milk of Livestock
Species .................................................... 629
Zsuzsanna Bösze and László Hiripi
Index ......................................................... 643
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