Preface .......................................................... v
Contributors .................................................... ix
1 Developing Extrachromosomal Gene Expression Vector
Technologies: An Overview ..................................... 1
Richard Wade-Martins
2 High Capacity Extrachromosomal Gene Expression Vectors ....... 19
Olivia Hibbitt and Richard Wade-Martins
3 Naturally Occurring Minichromosome Platforms in Chromosome
Engineering: An Overview ..................................... 41
Elena Raimondi
4 Chromosome Transfer Via Cell Fusion .......................... 57
Marianna Paulis
5 Insertional Engineering of Chromosomes with Sleeping
Beauty Transposition: An Overview ............................ 69
Ivana Grabundzija, Zsuzsanna Izsvdk, and Zoltán Ivies
6 'Rodent Transgenesis Mediated by a Novel Hyperactive
Sleeping Beauty Transposon System ............................ 87
Lajos Mátés
7 Construction and Use of a Bottom-Up НАС Vector for
Transgene Expression ........................................ 101
Masashi Ikeno and Nobutaka Suzuki
8 De Novo Generation of Satellite DNA-Based Artificial
Chromosomes by Induced Large-Scale Amplification ............ 111
Erika Csonka
9 Downstream Bioengineering of ACE Chromosomes for
Incorporation of Site-Specific Recombination Cassettes ...... 127
Amy L. Greene and Edward L. Perkins
10 Chromosome Engineering with Lambda-Integrase Mediated
Recombination System: The ACE System ........................ 141
Tünde Praznovszky
11 Dendrimer Mediated Transfer of Engineered Chromosomes ....... 151
Robert L. Katona
12 Engineered Chromosomes in Transgenics ....................... 161
Peter Blazso, Ildiko Sinko, and Robert L. Katona
13 Transfer of Stem Cells Carrying Engineered Chromosomes
with XY Clone Laser System .................................. 183
Ildiko Sinko and Robert L. Katona
14 Mammalian Artificial Chromosomes and Clinical Applications
for Genetic Modification of Stem Cells: An Overview ......... 199
Robert L. Katona, Sandra L. Vanderbyl, and Carl F. Perez
15 Engineered Mammalian Chromosomes in Cellular Protein
Production: Future Prospects ................................ 217
Malcolm L. Kennard
Index .......................................................... 239
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