Preface ......................................................... v
Contributors ................................................... ix
Part I Sampling and Experimental design
1 Sampling in Landscape Genomics ............................... 3
Stéphanie Mattel, Cécile H. Albert, and Nigel G. Yoccoz
2 OligoTag: A Program for Designing Sets of Tags for
Next-Generation Sequencing of Multiplexed Samples ........... 13
Eric Coissac
3 SNP Discovery in Non-model Organisms Using 454
Next Generation Sequencing .................................. 33
Christopher W. Wheat
4 In Silico Fingerprinting (ISIF): A User-Friendly In Silico
AFLP Program ................................................ 55
Margot Paris and Laurence Despres
Part II Producing Data
5 Diversity Arrays Technology: A Generic Genome Profiling
Technology on Open Platforms ................................ 67
Andrzej Kilian, Peter Wenzl, Eric Huttner, Jason Carling,
Ling Xia, Hélène Blots, Vanessa Caig, Katarzyna
Heller-Uszynska, Damian Jaccoud, Colleen Hopper,
Malgorzata Aschenbrenner-Kilian, Margaret Evers, Kaiman
Peng, Cyril Cayla, Puthick Hok, and Grzegorz Uszynski
6 Two Methods to Easily Obtain Nucleotide Sequences from
AFLP Loci of Interest ....................................... 91
Margot Paris, Claire-Lise Meyer, Christelle Blassiau, Eric
Coissac, Pierre Taberlet, and Laurence Desprès
7 Roche Genome Sequencer FLX Based High-Throughput
Sequencing of Ancient DNA .................................. 109
David E. Alquezar-Planas and Sarah L. Fordyce
8 Preparation of Normalized cDNA Libraries for 454
Titanium Transcriptome Sequencing .......................... 119
Zhao Lai, Yi Zou, Nolan C. Kane, Jeong-Hyeon Choi, Xinguo
Wang, and Loren H. Rieseberg
9 RAD Paired-End Sequencing for Local de Novo Assembly
and SNP Discovery in Non-model Organisms ................... 135
Paul D. Etter and Eric Johnson
Part III Analyzing Data
10 Automated Scoring of AFLPs Using RawGeno 2.0, a Free R
CRAN Library ............................................... 155
Nils Arrigo, Rolf Holderegger, and Nadir Alvarez
11 Haplotype Inference ........................................ 177
Olivier Delaneau and Jean-François Zagury
12 Allele Identification in Assembled Genomic Sequence
Datasets ................................................... 197
Katrina M. Dlugosch and Aurélie Bonin
13 Multiple Testing in Large-Scale Genetic Studies ............ 213
Matthieu Bouaziz, Marine Jeanmougin, and Mickaël Guedj
14 Population Genomic Analysis of Model and Nonmodel
Organisms Using Sequenced RAD Tags ......................... 235
Paul A. Hohenlohe, Julian Catchen, and William A. Cresko
15 Analysis and Management of Gene and Allelic Diversity in
Subdivided Populations Using the Software Program METAPOP .. 261
Andrés Pérez-Figueroa, Silvia T. Rodríguez-Ramilo, and
Armando Caballero
16 DetSel: An R-Package to Detect Marker Loci Responding to
Selection .................................................. 277
Renaud Vitalis
17 Use of Qualitative Environmental and Phenotypic Variables
in the Context of Allele Distribution Models: Detecting
Signatures of Selection in the Genome of Lake Victoria
Cichilds ................................................... 295
Stephane Joost, Michael Kalbermatten, Etienne Bezault,
and Ole Seehausen
18 Genomic Scan as a Tool for Assessing the Genetic
Component of Phenotypic Variance in Wild Populations ....... 315
Carlos M. Herrera
Index ......................................................... 331
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