Preface ....................................................... vii
Contributors ................................................... xi
1 Challenges for Assessing the Performance of Biomass
Degrading Biocatalysts ....................................... 1
Michael E. Himmel, Stephen R. Decker, and David K. Johnson
Part I Biomass Feedstock and Cellulose
2 Overview of Computer Modeling of Cellulose .................. 11
Malin Bergenstråhle-Wohlert and John W. Brady
3 Imaging Cellulose Using Atomic Force Microscopy ............. 23
Shi-You Ding and Yu-San Liu
4 Preservation and Preparation of Lignocellulosic Biomass
Samples for Multi-scale Microscopy Analysis ................. 31
Bryon S. Donohoe, Peter N. Ciesielski, and Todd B. Vinzant
5 Coherent Raman Microscopy Analysis of Plant Cell Walls ...... 49
Yining Zeng, Michael E. Himmel, and Shi-You Ding
6 Immunological Approaches to Plant Cell Wall and Biomass
Characterization: Glycome Profiling ......................... 61
Sivakumar Pattathil, Utku Avci, Jeffrey S. Miller, and
Michael G. Hahn
7 Immunological Approaches to Plant Cell Wall and Biomass
Characterization: Immunolocalization of Glycan Epitopes ..... 73
Utku Avci, Sivakumar Pattathil, and Michael G. Hahn
8 A Method to Evaluate Biomass Accessibility in Wet State
Based on Thermoporometry .................................... 83
Bon-Wook Koo and Sunkyu Park
Part II Plant Cell Wall
9 Cellulase Processivity ...................................... 93
David B. Wilson and Maxim Kostylev
10 A Simple Method for Determining Specificity of
Carbohydrate-Binding Modules for Purified and Crude
Insoluble Polysaccharide Substrates ........................ 101
Oren Yaniv, Sadanari Jindou, Felix Frolow, Raphael Lamed,
and Edward A. Bayer
11 Bacterial Cadherin Domains as Carbohydrate Binding
Modules: Determination of Affinity Constants to Insoluble
Complex Polysaccharides .................................... 109
Milana Fraiberg, Ilya Borovok, Ronald M. Weiner, Raphael
Lamed, and Edward A. Bayer
12 Affinity Electrophoresis as a Method for Determining
Substrate-Binding Specificity of Carbohydrate-Active
Enzymes for Soluble Polysaccharides ........................ 119
Sarah Moraïs, Raphael Lamed, and Edward A. Bayer
13 Single-Molecule Tracking of Carbohydrate-Binding Modules
on Cellulose Using Fluorescence Microscopy ................. 129
Tu-San Liu, Shi-You Ding, and Michael E. Himmel
14 Bioprospecting Metagenomics for New Glycoside Hydrolases ... 141
Jack Gilbert, Luen-Luen Li, Safiyh Taghavi,
Sean M. McCorkle, Susannah Tringe, and Daniel van
der Lelie
15 Anaerobic High-Throughput Cultivation Method for
Isolation of Thermophiles Using Biomass-Derived
Substrates ................................................. 153
Scott D. Hamilton-Brehm, Tatiana A. Vishnivetskaya,
Steve L. Allman, Jonathan R. Mielenz, and James G. Elkins
16 Assessing the Protein Concentration in Commercial Enzyme
Preparations ............................................... 169
William S. Adney, Nancy Dowe, Edward W. Jennings,
Ali Mohagheghi, John Yarbrough, and James D. McMillan
17 Reducing the Effect of Variable Starch Levels in Biomass
Recalcitrance Screening .................................... 181
Stephen R. Decker, Melissa Carlile, Michael J. Selig,
Crissa Doeppke, Mark Davis, Robert Sykes, Geoffrey
Turner, and Angela Ziebell
18 Analysis of Transgenic Glycoside Hydrolases Expressed in
Plants: T. reesei CBH I and A. cellulolyticus EI ........... 197
Roman Brunecky, John O. Baker, Hui Wei, Larry E. Taylor,
Michael E. Himmel, and Stephen R. Decker
Part III Lignins and Hemicelluloses
19 Structural Characterization of the Heteroxylans from
Poplar and Switchgrass ..................................... 215
Koushik Mazumder, Maria J. Реñа, Malcolm A. O'Neill,
and William S. York
20 Laser Microdissection and Genetic Manipulation
Technologies to Probe Lignin Heterogeneity and
Configuration in Plant Cell Walls .......................... 229
Oliver R.A. Corea, Chanyoung Ki, Claudia L. Cardenas,
Laurence B. Davin, and Norman G. Lewis
21 Lignin-Degrading Enzyme Activities ......................... 251
Yi-ru Chen, Simo Sarkanen, and Yun-Yan Wang
Index ......................................................... 269
|