Contributors .................................................. vii
Preface ...................................................... xiii
SECTION 1 Agrobacterium-Mediated Transformation
Chapter 1 Host Factors Involved in Genetic Transformation
of Plant Cells by Agrobacterium ...................... 3
BENOÎT LACROIX, ADI ZALTSMAN, AND VITALY CITOVSKY
Chapter 2 Genomics of Agrobacterium-Plant Interaction: An
Approach to Refine the Plant Transformation
Technology .......................................... 31
AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, AND
KIRANKUMAR S. MYSORE
SECTION 2 Other Transformation Technologies
Chapter 3 Particle Bombardment: An Established Weapon in
the Arsenal of Plant Biotechnologists ............... 53
THEODORE M. KLEIN
Chapter 4 A Novel Gene Delivery System in Plants with
Calcium Alginate Micro-Beads ........................ 73
NAOKI WADA, SHIN'ICHIRO KAJIYAMA, NARUEMON
KHEMKLADNGOEN, AND KIICHI FUKUI
Chapter 5 Pollen Transformation Technologies .................. 83
TATIANA RESCH AND ALISHER TOURAEV
Chapter 6 Intragenic Vectors and Marker-Free
Transformation: Tools for a Greener Biotechnology ... 93
CAIUS M. ROMMENS, ANTHONY CONNER, HUA YAN, AND
ZAC HANLEY
Chapter 7 Visualizing Transgene Expression ................... 109
JOHN J. FINER
SECTION 3 Vectors, Promoters, and Other Tools for Plant
Transformation
Chapter 8 Current State and Perspective of Binary Vectors
and Superbinary Vectors ............................ 123
TOSHIYUKI KOMORI AND TOSHIHIKO KOMARI
Chapter 9 Novel Dual Binary Vectors (pCLEAN) for Plant
Transformation ..................................... 139
PHILIPPE VAIN
Chapter 10 pORE Modular Vectors for Plant Transformation ...... 149
DWAYNE D. HEGEDUS
Chapter 11 pANIC: A Versatile Set of Gateway-Compatible
Vectors for Gene Overexpression and RNAi-
Mediated Down-Regulation in Monocots ............... 161
DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L.
ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL
STEWART, JR.
SECTION 4 Transgene Integration, Stability, Methylation,
Silencing
Chapter 12 Understanding and Avoiding Transgene Silencing ..... 171
MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R.
STERNES, AND ROBERT G. BIRCH
Chapter 13 Site-Specific Recombination for Precise and
"Clean" Transgene Integration in Plant Genome ...... 197
VIBHA SRIVASTAVA AND DAVID W. OW
SECTION 5 Selection Systems, Marker-Free Transformation
Chapter 14 Selectable Marker Genes: Types and Interactions .... 213
BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE,
SOUAD EL OUAKFAOUI, DOUGLAS A. JOHNSON,
LORETA GUDYNAITE-SAVITCH, MING HU, AND PHIL
MACDONALD
Chapter 15 Transformation Methods for Obtaining Marker-
Free Genetically Modified Plants ................... 229
JAN G. SCHAART, FRANS A. KRENS, ANNE-MARIE A.
WOLTERS, AND RICHARD G.F. VISSER
Chapter 16 Intellectual Property Aspects of Plant
Transformation ..................................... 243
JIM M. DUNWELL
Index ......................................................... 271
Color Plate between pages 82 and 83
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