Contributors ................................................... ix
Preface ...................................................... xiii
1 Evolution of DNA Marker Technology in Plants ................. 3
Robert J. Henry
Introduction ................................................. 4
Early Marker Technologies .................................... 4
Evolving Range of Applications of DNA Markers in Plants ..... 12
Applications ................................................ 13
Future Developments ......................................... 15
References .................................................. 15
2 Whole-Genome Sequencing for Marker Discovery ................ 21
Mark Edwards
Sequencing Strategies ....................................... 22
Sequencing Technologies ..................................... 23
Epigenetic Markers .......................................... 31
Genome-Wide Selection ....................................... 32
Data Analysis Resources ..................................... 32
References .................................................. 33
Color plate section located between pages 34 and 35.
3 Amplicon Sequencing for Marker Discovery .................... 35
Timothy R. Sexton and Frances M. Shapter
Introduction ................................................ 36
Background .................................................. 36
Maximizing Efficiency Through Sample Pooling ................ 38
Limitations of Amplicon-Based MPS ........................... 44
Bioinformatics .............................................. 51
Concluding Remarks .......................................... 52
Acknowledgments ............................................. 52
References .................................................. 53
4 Transcriptome Sequencing for Marker Discovery ............... 57
Susan Gillies
Introduction ................................................ 58
Basic Approach .............................................. 58
Conclusions ................................................. 64
References .................................................. 64
5 Molecular Markers in Plant Improvement ...................... 67
Peter J. Prentis, Edward K. Gilding, Ana Pavasovic, Celine
H. Frere, and Ian D. Godwin
Introduction ................................................ 68
Plant Domestication and Traditional Breeding ................ 68
Application of Molecular Markers to Breeding ................ 70
Next-Generation Approaches to QTL Discovery ................. 75
Conclusion .................................................. 77
References .................................................. 78
6 Applications of Molecular Markers in Plant Conservation ..... 81
Maurizio Rossetto and Paul D. Rymer
Introduction ................................................ 82
Traditional Approaches ...................................... 86
The Way Forward ............................................. 91
Conclusion .................................................. 95
References .................................................. 96
7 Molecular Markers for Plant Biosecurity ..................... 99
Andrew D.W. Geering
Introduction ............................................... 100
The Present - PCR for Specific Diagnosis and for DNA
Barcoding .................................................. 101
The Future - Next-Generation Sequencing Methods to
Revolutionize Plant Quarantine Diagnostics ................. 105
Conclusions ................................................ 110
Acknowledgments ............................................ 111
References ................................................. 111
8 Molecular Markers for Harnessing Heterosis ................. 119
Gopala S. Krishnan, A.K. Singh, Daniel L.E. Waters, and
Robert J. Henry
Introduction ............................................... 120
Molecular Markers for Understanding the Genetic Basis of
Heterosis .................................................. 122
Molecular Diversity and Heterosis — Molecular Markers for
Predicting Heterosis ....................................... 123
Conclusion ................................................. 131
References ................................................. 132
9 Genetic Variant Discovery and Its Use in Genome
Characterization of Agronomically Important Crop Species ... 137
Stéphane Deschamps and Matthew A. Campbell
Introduction ............................................... 138
Sanger Resequencing ........................................ 140
Single Feature Polymorphisms ............................... 140
Next-Generation Sequencing ................................. 143
High-Density Genotyping using the Illumina Golden Gate
Platform ................................................... 153
Genotyping by Sequencing ................................... 155
Genome Characterization and Haplotypes ..................... 157
Conclusions and Perspectives ............................... 159
References ................................................. 160
10 Future Prospects of Molecular Markers in Plants ............ 169
Reyazul R. Mir and Rajeev K. Varshney
Introduction ............................................... 170
Molecular Markers: The Past ................................ 172
Molecular Markers: The Present ............................. 173
V Molecular Markers: The Future ........................... 175
Novel Approaches or Platforms for Plant Breeding ........... 180
Conclusions ................................................ 183
Acknowledgments ............................................ 184
References ................................................. 184
Index ......................................................... 191
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