Plant stress tolerance: methods and protocols / ed. by R.Sunkar. - New York: Humana Press: Springer, 2010. - xiv, 386 p.: ill. - (Methods in molecular biology; 639) (Springer protocols). - Incl. bibl. ref. and ind. - ISBN 978-1-60761-701-3; ISSN 1064-3745  Место хранения:   05 | Сибирский институт физиологии и биохимии растений СО РАН | Иркутск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi Part I Reviews and Overview Chapters 1 Dehydration Tolerance in Plants .............................. 3 Melvin J. Oliver, John C. Cusbman, and Karen L. Koster 2 Approaches to Identifying Genes for Salinity Tolerance and the Importance of Timescale ............................. 25 Rana Munns 3 Gene Regulation During Cold Stress Acclimation in Plants .... 39 Viswanathan Chinnusamy, Jian-Kang Zhu, and Ramanjulu Sunkar 4 Redox-Dependent Regulation, Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress ........... 57 Karl-Josef Dietz 5 Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress ........ 71 Nese Sreenivasulu, Ramanjulu Sunkar, Ulrich Wobus, and Marc Strickert 6 Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants .................................. 95 Ananda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek, and Sudhir K. Sopory Part II Genome-Wide Approaches for Identification of Stress- Regulated Genes, Proteins, and Small RNAs 7 Genetic Screens to Identify Plant Stress Genes ............. 121 Csaba Papdi, Jeffrey Leung, Mary Prathiba Joseph, Imma Perez Salamу, and László Szabados 8 Arabidopsis Tiling Array Analysis to Identify the Stress- Responsive Genes ........................................... 141 Akihiro Matsui, Junko Ishida, Taeko Morosawa, Masanori Okamoto, Jong-Myong Kim, Yukio Kuribara, Makiko Kawasbima, Maho Tanaka, Taiko Kim To, Kentaro Nakaminami, Eli Kaminuma, Takaho A. Endo, Yoshiki Mochizuki, Shuji Kawaguchi, Norio Kobayashi, Kazuo Shinozaki, Tetsuro Toyoda, and Motoaki Seki 9 Identification of Stress-Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example ....................................... 157 Lila Peal, Michael Puckette, and Ramamurthy Mahalingam 10 Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen ..................................................... 171 Peter Klein and Karl-Josef Dietz 11 Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi ................................ 193 Muthappa Senthil-Kumar, Makarla Udayakumar, and Kirankumar S. Mysore 12 Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins .................................. 207 Jenny Renaut 13 Thiol-Disulfide Redox Proteomics in Plant Research ......... 219 Meenakumari Muthuramalingam, Karl-Josef Dietz, and Elke Ströher 14 Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants ........................................... 239 Jose Luis Reyes, Catalina Arenas-Huertero, and Ramanjulu Sunkar 15 An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants ........................................ 253 Xiaoyun Jia, Venugopal Men Ли, and Guiliang Tang Part III Antioxidant Enzymes and Metabolites 16 Spectrophotometric Assays for Antioxidant Enzymes in Plants ..................................................... 273 Sathya Elavarthi and Bjorn Martin 17 Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases ............................................. 281 Hans-Hubert Kirch and Horst Röhrig 18 Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants ..................................................... 291 Niranjani Jambunathan Part IV Osmotic Adjustment and Ion Measurements 19 Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies .................................. 301 Paul E. Verslues 20 Methods for Determination of Proline in Plants ............. 317 Edit Abraham, Cecile Hourton-Cabassa, László Erdei, and László Szabados 21 A New Method for Accurately MeasuringΔ1Pyrroline-5- Carboxylate Synthetase Activity ............................ 333 Elodie Parre, Jacques de Virville, Françoise Cochet, Anne-Sophie Leprince, Luc Richard, Delphine Lefebvre-De Vos, Mohamed Ali Ghars, Marianne Bordenave, Alain Zachowski, and Arnould Savouré 22 Extraction and Analysis of Soluble Carbohydrates ........... 341 Niels Maness 23 Measuring Soluble Ion Concentrations (Na+, K+, Cl-) in Salt-Treated Plants ..................................... 371 Rana Munns, Patricia A. Wallace, Natasha L. Teakle, and Timothy D. Colmer Subject Index ......................................................... 383