Mouse cell culture: methods and protocols / ed. by A.Ward, D.Tosh. - New York: Humana Press, 2010. - xi, 257 p.: ill. (some col.). - (Methods in molecular biology; 633) (Springer protocols). - ISBN 978-1-58829-772-3; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies ........................................... 1 Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester 2 Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures .................................................... 19 Alastair S. Garfield 3 Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells ..................... 29 Janet Smith and Deborah Merrick 4 The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference ......................... 57 Jamie A. Davies 5 Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation ............................................. 71 Pierre-Marie del Moral and David Warburton 6 Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine .......................... 81 Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh 7 Isolation and Culture of Embryonic Pancreas and Liver ....... 91 Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh 8 Isolation and Culture of Mouse Satellite Cells ............. 101 Antonio Musarò and Laura Barberi 9 Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures ........................................... 113 Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton 10 Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes .............................. 125 Reto Caldelari and Eliane J. Müller 11 Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells ........................................... 139 Matthew J. Smalley 12 Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes ......................................... 171 Magalie A. Ravier and Guy A. Rutter 13 Isolation and Culture of Adult Mouse Hepatocytes ........... 185 Wan-Chun Li, Kate L. Ralphs, and David Tosh 14 Isolation and Culture of Mouse Intestinal Cells ............ 197 Charles Frederick Campbell 15 Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse ............................................. 207 Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward 16 The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry .............................................. 221 Carla Sciarretta and Liliana Minichiello 17 Cell Culture of Primary Cerebellar Granule Cells ........... 233 Dana Krämer and Liliana Minichiello 18 Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain ............................ 241 Henrik Ahlenius and Zaal Kokaia Subject Index ................................................. 253