Nucleic acid and peptide aptamers: methods and protocols / ed. by G.Mayer. - New York: Humana, 2009. - xi, 408 p.: ill. - Incl. bibl. ref. - Ind.: p.405-408. - ISBN 978-1-934115-89-3  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix Part I: Nucleic Acid Aptamers 1 Nucleic Acid Pool Preparation and Characterization ........... 3 Shawn K. Piasecki, Bradley Hall, and Andrew D. Ellington 2 In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins ............................................. 19 Günter Mayer and Thomas Höver 3 Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX) .................................................. 33 Renee K. Mosing and Michael T. Bowser 4 In Vitro Selection of Allosteric Ribozymes .................. 45 Nicolas Piganeau 5 Cell-Specific Aptamers for Targeted Therapies ............... 59 Laura Cerchia, Paloma H. Giangrande, James O. McNamara, and Vittorio de Franciscis 6 Aptamers Targeting RNA Molecules ............................ 79 Marguerite Watrin, Eric Dausse, Isabelle Lebars, Bernard Rayner, Anthony Bugaut, and Jean-Jacques Toulmé 7 Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction ............. 107 Arne Werner and Ulrich Hahn 8 Structural Probing Techniques on Natural Aptamers .......... 115 Catherine A. Wakeman and Wade С. Winkler 9 Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography ................................... 135 Andrea L. Edwards, Andrew D. Garst, and Robert T. Batey 10 Locked Nucleic Acid Aptamers ............................... 165 Jan Barciszewski, Michael Medgaard, Troels Koch, Jens Kurreck, and Volker A. Erdmann 11 Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay ............... 187 Satoko Yamazaki and Michael Famulok 12 Aptamers as Artificial Gene Regulation Elements ............ 201 Beatrix Suess and Julia E. Weigand 13 Aptamers and Biosensors .................................... 209 Thomas M.A. Gronewold 14 Nanoparticles/Dip Stick .................................... 223 Yi Lu, Juewen Liu and Debapriya Mazumdar 15 In Vivo Imaging of Oligonucleotidic Aptamers ............... 241 Bertrand Tavitian, Frédéric Ducongé, Raphael Boisgard, and Frédéric Dollé Part II: Peptide Aptamers 16 Selection of Peptides Interfering with Protein-Protein Interaction ................................................ 263 Annette Gaida, Urs B. Hagemann, Dinah Mattay, Christina Räuber, Kristian M. Müller, and Katja M. Arndt 17 In Vitro Selection of Protein and Peptide Libraries Using mRNA Display ............................................... 293 Terry T. Takahashi and Richard W. Roberts 18 Ligand-Regulated Peptide Aptamers .......................... 315 Russell A. Miller 19 Isolation of Peptide Aptamers to Target Protein Function ... 333 Luisa Lopez-Ochoa, Тага Е. Nash, Jorge Ramirez-Prado, and Linda Hanley-Bowdoin 20 MicrobodiesTM .............................................. 361 Hans-Ulrich Schmoldt, Matin Daneschdar, Harald Kolmar, and Michael Blind 21 Peptide Aptamers for Small Molecule Drug Discovery ......... 373 Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, and Pierre Colas 22 Synthesis and Application of Peptides as Drug Carriers ..... 389 Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger Index ......................................................... 405