Plant signal transduction: methods and protocols / ed. by T.Pfannschmidt. - New York: Humana Press, 2009. - xi, 357 p., [8] p. of plates ill. (some col.). - (Springer protocols) (Methods in molecular biology; 479). - Incl. bibl. ref. - Ind.: p.353-357. - ISBN 978-1-58829-943-7  Место хранения:   05 | Сибирский институт физиологии и биохимии растений СО РАН | Иркутск

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... ix 1 Multiple Dimensions in Plant Signal Transduction: An Overview ..................................................... 1 Jorge J. Casal 2 Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions ................................... 17 Cordelia Bolle 3 Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis .............................................. 35 Cordelia Bolle 4 Accurate Real-time Reverse Transcription Quantitative PCR ... 61 Marco Klatte and Petra Bauer 5 Probing Spatio-temporal Intracellular Calcium Variations in Plants ................................................... 79 Axel Mithöfer, Christian Mazars, and Massimo E. Maffei 6 Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy ................ 93 Andreas J. Meyer and Thorsten Brach 7 Imaging of Reactive Oxygen Species in vivo ................. 109 Steven M. Driever, Michael J. Fryer, Philip M. Mullineaux, and Neil R. Baker 8 Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single Cysteine Mutant-immobilized Resin ....... 117 Ken Motohashi, Patrick G.N. Romano, and Toru Hisabori 9 Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes ................................... 133 Julia P. Vainonen, Alexander V. Vener, and Eva-Mari Aro 10 Examining Protein Stability and Its Relevance for Plant Growth and Development ..................................... 147 Claus Schwechheimer, Björn C. Willige, Melina Zourelidou, and Esther M.N. Dohmann 11 Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics ................................... 173 Volker Wagner and Maria Mittag 12 Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells ......... 189 Katia Schütze, Klaus Harter, and Christina Chaban 13 Fluorescence Cross-Correlation Spectroscopy of Plant Proteins ................................................... 203 Hideki Muto, Masataka Kinjo, and Kotaro T. Yamamoto 14 The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin-system (mbSUS) ................ 217 Christopher Grefen, Petr Obrdlik, and Klaus Harter 15 Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways .................................. 235 Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil, and Markus Teige 16 Phosphatase Activities Analyzed by in vivo Expressions ..... 247 Alois Schweighofer, Zahra Ayatollahi, and Irute Meskiene 17 Chromatin Immunoprecipitation Experiments to Investigate in vivo Binding of Arabidopsis Transcription Factors to Target Sequences ........................................... 261 Benjamin Fode and Christiane Gatz 18 Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins .................... 273 Sebastian Steiner and Thomas Pfannschmidt 19 Analysis of Plant Regulatory DNA sequences by the Yeast- One-Hybrid Assay ........................................... 291 Dierk Wanke and Klaus Harter 20 Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation ... 311 Kenneth W. Berendzen, Klaus Harter, and Dierk Wanke 21 RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System ......................... 337 Jan C. Schöning and Dorothee Staiger Index ......................................................... 353