Molecular beacons: signalling nucleic acid probes, methods, and protocols / ed. by A.Marx, O.Seitz. - Totowa: Humana Press, 2008. - xii, 266 p.: ill. (some col.). - (Methods in molecular biology; 429). - Incl. bibl. ref. - Ind.: p.265-266. - ISBN 978-1-58829-700-6  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ...................................................... v Contributors ................................................ xi I Introduction 1 Basic Principles of Fluorescence and Energy Transfer ......... 3 Larry E. Morrison II Applications 2 Multiplex Detection of Mutations ............................ 23 David S. Perlin, Sergey Balashov, and Steven Park 3 Detection of Salmonella Species in Foodstuffs ............... 33 Arvind A. Bhagwat, Jitu Patel, Trina Chua, Audrey Chan, Saúl Ruiz Cruz, and Gustavo A. González Aguilar 4 Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR ............................................... 45 Gunter Schmidtke and Marcus Groettrup 5 Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR Greenl-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma ............... 59 Sebastian Lukasiak, Kai Breuhahn, Claudia Schiller, Gunter Schmidtke, and Marcus Groettrup 6 Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-Time PCR and RT-PCR ...................................................... 73 Valentino Cattori and Regina Hofmann-Lehmann 7 High-Throughput Real-Time PCR ............................... 89 Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang 8 Design and Use of Scorpions Fluorescent Signalling Molecules ................................................... 99 Rachael Carters, Jennifer Ferguson, Rupert Gaut, Paul Ravetto, Nicola Thelwell, and David Whitcombe 9 Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification ..... 117 Thomas Froehlich and Oliver Geulen III New Technologies 10 EasyBeaconsTM for the Detection of Methylation Status of Single CpG Duplets ...................................... 137 Ulf B. Christensen 11 Quenched Autoligation Probes ............................... 161 Adam P. Silverman, Hiroshi Abe, and Eric T. Kool 12 HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination ...................................... 171 David J. French, David G. McDowell, Paul Debenham, Nittaya Gale, and Tom Brown 13 FIT-Probes in Real-Time PCR ................................ 187 Elke Socher and Oliver Seitz 14 SNP Genotyping by Unlabeled Probe Melting Analysis ......... 199 Maria Erali, Robert Palais, and Carl Wittwer IV Non-Nucleic Acid Targets 15 Molecular Beacons for Protein —DNA Interaction Studies ..... 209 Jun Li, Zehui Charles Cao, Zhiwen Tang, Kemin Wang, and Weihong Tan 16 DNA Polymerase Profiling ................................... 225 D. Summerer 17 Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions ..................................... 237 S. Hani Najafi-Shoushtari and Michael Famulok 18 Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes ....................................... 251 Jörg S. Hartig and Michael Famulok Index ...................................................... 265