Reich E. High-performance thin-layer chromatography for the analysis of medicinal plants / E.Reich, A.Schibli. - New York; Stuttgart: Thieme, 2007. - xv, 264 p.: ill. - Incl. bibl. ref. - Ind.: p.259-264. - ISBN 1-58890-409-1; ISBN 978-1-58890-409-6 (US); ISBN 3-13-141601-7; ISBN 978-3-13-141601-8 (GTV)  Место хранения:   03 | Центральная библиотека Иркутского научного центра СО РАН | Иркутск

Оглавление / Contents

Foreword ....................................................... xi Preface ...................................................... xiii Acknowledgments ................................................ xv 1 Introduction ................................................. 1 Analysis of Herbal Drugs ..................................... 1 What Is Chromatography? ...................................... 6 What Is TLC? ................................................ 11 Modern TLC .................................................. 19 2 Theoretical Concepts ........................................ 22 General Chromatographic Principles .......................... 22 Special Aspects of TLC ...................................... 33 The Stationary Phase ........................................ 42 The Mobile Phase ............................................ 48 The Chromatographic Result in TLC ........................... 55 Densitometry ................................................ 61 3 Practical Aspects of Modern TLC ............................. 67 Sample Preparation .......................................... 68 Selecting the Stationary Phase .............................. 73 Sample Application .......................................... 92 Chromatogram Development .................................... 97 Derivatization ............................................. 110 Visualization and Documentation of the Chromatogram ........ 114 Densitometry ............................................... 116 Reporting and Record Keeping ............................... 121 TLC Software ............................................... 125 Standardization ............................................ 125 Practical Recommendations .................................. 127 4 Typical Applications in Herbal Analysis .................... 129 Why HPTLC? ................................................. 129 HPTLC Analysis of Raw Material ............................. 132 HPTLC Analysis of Herbal Drug Preparations and Products .... 160 5 Method Development ......................................... 175 Theoretical Aspects ........................................ 175 Method Development in Practice ............................. 179 6 Validation ................................................. 193 General Information ........................................ 193 Validation of Qualitative HPTLC Methods .................... 206 Validation of Quantitative HTPLC Methods ................... 210 The Final Word ............................................. 214 List of Abbreviations ......................................... 215 Glossary of TLC Terms ......................................... 218 Appendixes A Definitions .............................................. 224 В Standard Operating Procedure for HPTLC of Herbal Raw Materials and Preparations ............................... 227 С Resources ................................................ 231 D Common Mobile Phases for the Screening of Unknown Herbal Drugs ............................................. 233 E Most Common Nonspecific Derivation Reagents for Analysis of Herbal Drugs ................................. 234 F Worksheet ................................................ 238 G Validation of Method for Identification of Eleuthero by HPTLC Fingerprint ........................................ 240 Index ......................................................... 259