Handbook of cryo-preparation methods for electron microscopy / ed. by A.Cavalier, D.Spehner, B.M.Humbel. - Boca Raton: CRC Press, 2009. - xix, 682 p.: ill. - (Methods in visualization). - Incl. bibl. ref. - Ind.: p.675-682. - ISBN 978-0-8493-7227-8  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Introduction ................................................. XVII Abbreviations ................................................. XIX Chapter 1 ....................................................... 1 Vitreous Water Jacques Dubochet Part I - Cryo-Fixation Methods Chapter 2 ...................................................... 17 Slam-Freezing, Metal-Mirror Freezing Danièle Spehner and Ludwig Edelmann Chapter 3 ...................................................... 49 Plunge-Freezing (Holey Carbon Method) Sacha De Carlo Chapter 4 ...................................................... 69 Controlled Vitrification Peter M. Frederik, Felix de Haas and Marc M.H. Storms Chapter 5 ..................................................... 101 BAL-TEC HPM 010 High-Pressure Freezing Machine Andres Kaech Chapter 6 ..................................................... 129 High-Pressure Freezing LEICA EMPACT Dimitri Vanhecke and Daniel Studer Part II - Cryo-Electron Microscopy Chapter 7 ..................................................... 159 Frozen-Hydrated Macromolecules for Structural Analysis Corinne Crucifix, Gabor Papai and Patrick Schultz Chapter 8 ..................................................... 191 Two-Dimensional Crystals Patrick Bron and Janet Vonck Chapter 9 ..................................................... 219 Cryo-Negative Staining Sacha De Carlo Chapter 10 .................................................... 237 Vitrification of Dynamic Microtubules Isabelle Arnal, Marine Malleter and Denis Chrétien Chapter 11 .................................................... 259 CEMOVIS: Cryo-Electron Microscopy of Vitreous Sections Jacques Dubochet, Ashraf Al-Amoudi, Cédric Вouchet- Marquis, Mikhail Eltsov and Benoît Zuber Chapter 12 .................................................... 291 Cryo-Electron Tomography Andrew Leis, Manuela Gruska and Wolfgang Baumeister Part III - Low-Temperature Embedding Chapter 13 .................................................... 319 Freeze-Substitution Bruno M. Humbel Chapter 14 .................................................... 343 Cryo-Fixation, Freeze-Substitution, Rehydration and Tokuyasu Cryo-Sectioning York-Dieter Stierhof, Elly van Donselaar, Heinz Schwarz, Bruno M. Humbel Chapter 15 .................................................... 367 Freeze-Drying and Embedding of Biological Material Ludwig Edelmann Part IV - Freeze-Fracture and Metal Shadowing Chapter 16 .................................................... 391 The Shadow of Hydrated Biological Specimens Daniel Thomas Chapter 17 .................................................... 411 Cryo-Fracture of Self-Assembled Systems in Organic Solvent Marc Schmutz and Philippe J. Mésini Part V - Analysis Chapter 18 .................................................... 433 Progressive Lowering of Temperature for Immunolabeling and in situ Hybridization Annie Cavalier and Danièle Spehner Chapter 19 .................................................... 467 Cryo-Sectioning According to Tokuyasu Bruno M. Humbel and York-Dieter Stierhof Chapter 20 .................................................... 499 Cryo-Preparation Procedures for Elemental Imaging by SIMS and EFTEM Christian Bordat and Jean-Luc Guerquin-Kern Chapter 21 .................................................... 537 Correlative Light and Electron Microscopy Heinz Schwarz and Bruno M. Humbel Chapter 22 .................................................... 567 SDS-digested Freeze-Fracture Replica Labeling (SDS-FRL) Yugo Fukazawa, Miwako Masugi-Tokita, Etsuko Tarusawa, Akari Hagiwara and Ryuichi Shigemoto Chapter 23 .................................................... 587 Immunolabeling of Ultrathin Sections with Enlarged 1 nm Gold or QDots York-Dieter Stierhof Chapter 24 .................................................... 617 3-D Electron Tomography of Cells and Organelles Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij Final Considerations .......................................... 651 Hellmuth Sitte Glossary ...................................................... 657 Index ......................................................... 673