Introduction: Principles of Electron Tomography ................. 1
Joachim Frank
Chapter 1
Sample Shrinkage and Radiation Damage of Plastic Sections ...... 17
Pradeep K. Luther
Chapter 2
Electron Tomography of Frozen-hydrated Sections of Cells
and Tissues .................................................... 49
Michael Marko, Chyong-Ere Hsieh and Carmen A. Mannella
Chapter 3
The Electron Microscope as a Structure Projector ............... 83
Peter W. Hawkes
Chapter 4
Cryotomography: Low-dose Automated Tomography of Frozen-
hydrated Specimens ............................................ 113
Abraham J. Koster and Montserrat Barcena
Chapter 5
Fiducial Marker and Hybrid Alignment Methods for Single-
and Double-axis Tomography .................................... 163
David N. Mastronarde
Chapter 6
Markerless Alignment in Electron Tomography ................... 187
Sami S. Brandt
Chapter 7
Algorithms for Three-dimensional Reconstruction From the
Imperfect Projection. Data Provided by Electron Microscopy .... 217
Jose-Maria Carazo, Gabor T. Herman, Carlos O.S. Sorzano
and Roberto Marabini
Chapter 8
Weighted Back-projection Methods .............................. 245
Michael Radermacher
Chapter 9
Reconstruction with Orthogonal Functions ...................... 275
Elmar Zeitler
Chapter 10
Resolution in Electron Tomography ............................. 307
Pawel A. Penczek and Joachim Frank
Chapter 11
Denoising of Electron Tomograms ............................... 331
Reiner Hegerl and Achilleas S. Frangakis
Chapter 12
Segmentation of Three-dimensional Electron Tomographic
Images ........................................................ 353
Achilleas S. Frangakis and Reiner Hegerl
Chapter 13
Segmentation of Cell Components Using Prior Knowledge ......... 371
Ming Jiang, Qiang Ji, Xun Wang and Bruce F. McEwen
Chapter 14
Motif Search in Electron Tomography ........................... 401
Achilleas S. Frangakis and Bimal K. Rath
Chapter 15
Localization and Classification of Repetitive Structures in
Electron Tomograms of Paracrystalline Assemblies .............. 417
Kenneth A. Taylor, Jun Liu and Hanspeter Winkler
Index ......................................................... 441
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