Avidin-biotin interactions: methods and applications / ed. by McMahon R.J. - Totowa: Humana, 2008. - xiii, 232 p.: ill. - (Methods in molecular biology; 418). - Ind.: p.221-232. - ISBN 978-1-58829-583-5; ISSN 1064-3745  Место хранения:   056 | Институт химической биологии и фундаментальной медицины CO РАН | Новосибирск| Библиотека

Оглавление / Contents

Preface ......................................................... v Contributors ................................................... xi 1. Preparation of Avidin Conjugates Rosaria P. Haugland and Mahesh K. Bhalgat ................ 7 2. Coupling of Antibodies With Biotin Rosaria P. Haugland and Wendy W. You .................... 13 3. Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin Sara R. Zwart and Brandon J. Lewis ...................... 25 4. Use of the Avidin (Imino) Biotin System as a General Approach to Affinity Precipitation Ruth Freitag and Frank Hilbrig .......................... 35 5. Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed Salman Lateef, and Shalini Gupta ................... 51 6. Functionality Screen of Streptavidin Mutantsby Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein Nicolas Humbert and Thomas R. Ward ...................... 63 7. Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives Andreas Ebner, Markus Marek, Karl Kaiser, Gerald Kada, Christoph D. Hahn, Bernd Lackner, and Hermann J. Gruber ....................................... 73 8. Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins Christoph Rösli, Jascha-N. Rybak, Dario Neri, and Giuliano Elia ....................................... 89 9. High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli Nicolas Humbert, Peter Schürmann, Andrea Zocchi, Jean-Marc Neuhaus, and Thomas R. Ward .................. 101 10. Endogenous Biotin in Rat Brain: Implications for False-Positive Results With Avidin-Biotin and Strepta vidin-Biotin Techniques Bruce E. McKay, Michael L Molineux, and Ray W. Turner ... 111 11. Pseudo-lmmunolabelling With the Avidin-Biotin- Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina Willem Kamphuis and Jan Klooster ....................... 129 12. Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones Gabriela Camporeale, Yap Ching Chew, Alice Kueh, Gautam Sarath, and Janos Zempleni ...................... 139 13. Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay Kirsten Liebert and Albert Jeltsch ..................... 149 14. Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux .......................................... 157 15. Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure Karel Smetana, Jr., and Sabine André ................... 171 16. A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin- Phycoerythrin Krista McCutcheon ...................................... 187 17. Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications Donald M. Mock and Anna Bogusiewicz .................... 209 Index ......................................................... 221