Preface ......................................................... v
Contributors ................................................... ix
1. Overview of Membrane Rafts
Thomas J. Mclntosh ........................................ 7
2. Analysis of Raft Affinity of Membrane Proteins by
Detergent-Insolubility
Deborah A. Brown .......................................... 9
3. Nondetergent Isolation of Rafts
Mehul B. Shah and Pravin B. Sehgal ....................... 21
4. Detecting Ordered Domain Formation (Lipid Rafts)
in Model Membranes Using Tempo
Omar Bakht and Erwin London .............................. 29
5. Using Monomolecular Films to Characterize Lipid
Lateral Interactions
Rhoderick E. Brown and Howard L. Brockman ................ 41
6. Electro-Formation and Fluorescence Microscopy of Giant
Vesicles With Coexisting Liquid Phases
Sarah L Veatch ........................................... 59
7. Fluorescence Correlation Spectroscopy
Kirsten Bacia and Petra Schwille ......................... 73
8. Multiphoton Laser-Scanning Microscopy and Spatial Analysis
of Dehydroergosterol Distributions on Plasma Membrane
of Living Cells
Avery L. Mclntosh, Barbara P. Atshaves, Huan Huang,
Adalberto M. Gallegos, Ann B. Kier, Friedhelm
Schroeder, Hal Xu, Weimin Zhang, Suojin Wang, and
Jyh-Charn Liu ............................................ 85
9. NMR Detection of Lipid Domains
Ivan V. Polozov and Klaus Gawrisch ...................... 107
10.Lateral Diffusion Coefficients of Raft Lipids From
Pulsed Field Gradient NMR
Greger Orädd and Göran Lindblom ......................... 127
11.Saturation-Recovery Electron Paramagnetic Resonance
Discrimination by Oxygen Transport (DOT) Method for
Characterizing
Membrane Domains Witold K. Subczynski, Justyna
Widomska, Anna Wisniewska, and Akihiro Kusumi ........... 143
12.Plasmon-Waveguide Resonance Spectroscopy Studies of
Lateral Segregation in Solid-Supported Proteolipid
Bilayers
Zdzislaw Salamon, Savitha Devanathan, and Gordon
Tollin .................................................. 159
13. Fluorescence Recovery After Photobleaching Studies
of Lipid Rafts
Anne K. Kenworthy ....................................... 179
14.Single-Molecule Tracking
Marija Vrljic, Stefanie Y. Nishimura, and
W.E. Moerner ............................................ 193
15.X-Ray Diffraction to Determine the Thickness of Raft
and Nonraft Bilayers
Thomas J. Mclntosh ...................................... 221
16.Small-Angle Neutron Scattering to Detect Rafts and Lipid
Domains
Jeremy Pencer, Thalia T. Mills, Norbert Kucerka,
Мu-Ping Nieh, and John Katsaras ......................... 231
17.Exploring Membrane Domains Using Native Membrane Sheets
and Transmission Electron Microscopy
Bridget S. Wilson, Janet R. Pfeiffer, Mary Ann
Raymond-Stintz, Diane lidke, Nicholas Andrews, Jun
Zhang, Wenxia Yin, Stanly Steinberg, and Janet
M. Oliver ............................................... 245
18.Atomic Force Microscopy of Lipid Domains
in Supported Model Membranes
Alan R. Burns ........................................... 263
19.Atomistic and Coarse-Grained Computer Simulations
of Raft-Like Lipid Mixtures
Sagar A. Pandit and H. Larry Scott ...................... 283
20.A Microscopic Model Calculation of the Phase Diagram
of Ternary Mixtures of Cholesterol and Saturated
and Unsaturated Phospholipids
R. Elliott, I. Szleifer, and M. Schick .................. 303
Index ......................................................... 319
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