Cell biology: a laboratory handbook. - 3rd ed. Vol.3 / ed. by Celis J. - Amsterdam; Boston: Elsevier Academic Press., 2006. - xlii, 527 p. - ISBN 10 0-12-164733-1; ISBN 3-978-0-164733-9  Место хранения:   040 | Институт биофизики СО РАН | Красноярск | Библиотека

Оглавление / Contents

Contents of other Volumes ...................................... xi Contributors ................................................ xxiii Preface ....................................................... xiv PART A. IMAGING TECHNIQUES Section 1. Light Microscopy 1. Fluorescence Microscopy ...................................... 5 WERNER BASCHONG AND LUKAS LANDMANN 2. Total Internal Reflection Fluorescent Microscopy ............ 19 DEREK TOOMRE AND DANIEL AXELROD 3. Band Limit and Appropriate Sampling in Microscopy ........... 29 RAINER HEINTZMANN 4. Optical Tweezers: Application to the Study of Motor Proteins .................................................... 37 WALTER STEFFEN, ALEXANDRE LEWALLE, AND JOHN SLEEP Section 2. Digital Video Microscopy 5. An Introduction to Electronic Image Acquisition during Light Microscopic Observation of Biological Specimens ....... 49 JENNIFER С WATERS 6. Video-Enhanced Contrast Microscopy .......................... 57 DIETER G. WEISS Section 3. Confocal Microscopy of Living Cells and Fixed Cells 7. Spinning Disc Confocal Microscopy of Living Cells ........... 69 TIMO ZIMMERMANN AND DAMIEN BRUNNER 8. Confocal Microscopy of Drosophila Embryos ................... 77 MAITHREYI NARASIMHA AND NICHOLAS H. BROWN 9. Ultraviolet Laser Microbeam for Dissection of Drosophila Embryos ..................................................... 87 DANIEL P. KIEHART, YOICHIRO TOKUTAKE, MING-SHIEN CHANG, M. SHANE HUTSON, JOHN WIEMANN, XOMALIN G. PERALTA, YUSUKE TOYAMA, ADRIENNE R. WELLS, ALICE RODRIGUEZ, AND GLENN S. EDWARDS Section 4. Fluorescent Microscopy of Living Cells 10.Introduction to Fluorescence Imaging of Live Cells: An Annotated Checklist ..................................... 107 YU-LI WANG 11.Cytoskeleton Proteins ...................................... 111 KLEMENS ROTTNER, IRINA N. KAVERINA, AND THERESIA E.B. STRADAL 12.Systematic Subcellular Localization of Novel Proteins ...... 121 JEREMY С SIMPSON AND RAINER PEPPERKOK 13.Single Molecule Imaging in Living Cells by Total Internal Reflection Fluorescence Microscopy ................ 129 ADAM DOUGLASS AND RONALD VALE 14.Live-Cell Fluorescent Speckle Microscopy of Actin Cytoskeletal Dynamics and Their Perturbation by Drug Perfusion ............................................. 137 STEPHANIE L. GUPTON AND CLARE M. WATERMAN-STORER 15.Imaging Fluorescence Resonance Energy Transfer between Green Fluorescent Protein Variants in Live Cells ........... 153 PETER J. VERVEER, MARTIN OFFTERDINGER, AND PHILIPPE I.H. BASTIAENS Section 5. Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters 16.Measurements of Endosomal pH inLive Cells by Dual- Excitation Fluorescence Imaging ............................ 163 NICOLAS DEMAUREX AND SERGIO GRINSTEIN 17.Genome-Wide Screening of Intracellular Protein Localization in Fission Yeast .............................. 171 DA-QIAO DING AND YASUSHI HIRAOKA 18.Large-Scale Protein Localization in Yeast .................. 179 ANUJ KUMAR AND MICHAEL SNYDER Section 6. Digital Image Processing, Analysis, Storage, and Display 19.Lifting the Fog: Image Restoration by Decon volution ....... 187 RICHARD M. PARTON AND ILAN DAVIS 20.The State of the Art in Biological Image Analysis .......... 201 FEDERICO FEDERICI, SILVIA SCAGLIONE, AND ALBERTO DIASPRO 21.Publishing and Finding Images in the Biolmage Database, an Image Database for Biologists ........................... 207 CHRIS CATTON, SIMON SPARKS, AND DAVID M. SHOTTON PART B. ELECTRON MICROSCOPY Section 7. Specimen Preparation Techniques 22.Fixation and Embedding of Cells and Tissues for Transmission Electron Microscopy ........................... 221 ARVID B. MAUNSBACH 23.Negative Staining .......................................... 233 WERNER BASCHONG AND UELI AEBI 24.Glycerol Spraying/Low-Angle Rotary Metal Shadowing ......... 241 UELI AEBI AND WERNER BASCHONG Section 8. Cryotechniques 25.Rapid Freezing of Biological Specimens for Freeze Fracture and Deep Etching .................................. 249 NICHOLAS J. SEVERS AND DAVID M. SHOTTON 26.Freeze Fracture and Freeze Etching ......................... 257 DAVID M. SHOTTON Section 9. Electron Microscopy Studies of the Cytoskeleton 27.Electron Microscopy of Extracted Cytoskeletons: Negative Staining, Cryoelectron Microscopy, and Correlation with Light Microscopy .......................... 267 GUENTER P. RESCH, J. VICTOR SMALL, AND KENNETH N. GOLDIE 28.Correlative Light and Electron Microscopy of the Cytoskeleton ............................................... 277 TATYANA M. SVITKINA AND GARY G. BORISY Section 10. Immunoelectron Microscopy 29.Immunoelectron Microscopy with Lowicryl Resins ............. 289 ARVID B. MAUNSBACH 30.Use of Ultrathin Cryo- and Plastic Sections for Immunocytochemistry ........................................ 299 NORBERT ROOS, PAUL WEBSTER, AND GARETH GRIFFITHS 31.Direct Immunogold Labeling of Components within Protein Complexes .......................................... 307 JULIE L. HODGKINSON AND WALTER STEFFEN PART C. SCANNING PROBE AND SCANNING ELECTRON MICROSCOPY Section 11. Scanning Probe and Scanning Electron Microscopy 32.Atomic Force Microscopy in Biology ......................... 317 DIMITRIOS FOTIADIS, PATRICK L.T.M. FREDERIX, AND ANDREAS ENGEL 33.Field Emission Scanning Electron Microscopy and Visualization of the Cell Interior ......................... 325 TERENCE ALLEN, SANDRA RUTHERFORD, STEVE MURRAY, SIEGFREID REIPERT, AND MARTIN GOLDBERG PART D. MICRODISSECTION Section 12. Tissue and Chromosome Microdissection 34.Laser Capture Microdissection .............................. 339 VIRGINIA ESPINA AND LANCE LIOTTA 35.Chromosome Microdissection Using Conventional Methods ...... 345 NANCY WANG, LIQIONG LI, AND HARINDRA R. ABEYSINGHE 36.Micromanipulation of Chromosomes and the Mitotic Spindle Using Laser Microsurgery (Laser Scissors) and Laser- Induced Optical Forces (Laser Tweezers) .................... 351 MICHAEL W. BERNS, ELLIOT BOTVINICK, LIH-HUEI LIAW, CHUNG-HO SUN, AND JAGESH SHAH PART E. TISSUE ARRAYS Section 13. Tissue Arrays 37.Tissue Microarrays ......................................... 369 RONALD SIMON, MARTINA MIRLACHER, AND GUIDO SAUTER PART F. CYTOGENETICS AND IN SITU HYBRIDIZATION Section 14. Cytogenetics 38.Basic Cytogenetic Techniques: Culturing, Slide Making, and G Banding .............................................. 381 KIM SMITH 39.A General and Reliable Method for Obtaining High-Yield Metaphasic Preparations from Adherent Cell Lines: Rapid Verification of Cell Chromosomal Content ............. 387 DORIS CASSIO Section 15. In Situ Hybridization 40.Mapping Cloned DNA on Metaphase Chromosomes Using Fluorescence in Situ Hybridization ......................... 395 MARGARET LEVERSHA 41.Human Genome Project Resources for Breakpoint Mapping ...... 403 DEBORAH С BURFORD, SUSAN M. GRIBBLE, AND ELENA PRIGMORE 42.Fine Mapping of Gene Ordering by Elongated Chromosome Methods .................................................... 409 THOMAS HAAF 43.In Situ Hybridization Applicable to mRNA Species in Cultured Cells ............................................. 413 ROELAND W. DIRKS 44.In Situ Hybridization for Simultaneous Detection of DNA, RNA, and Protein ........................................... 419 NOELIA CUSTODIO, CELIA CARVALHO, T. CARNEIRO, AND MARIA CARMO-FONSECA 45.Fluorescent Visualization of Genomic Structure and DNA Replication at the Single Molecule Level ................... 429 RONALD LEBOFSKY AND AARON BENSIMON PART G. GENOMICS Section 16. Genomics 46.Genomic DNA Microarray for Comparative Genomic Hybridization .............................................. 445 ANTOINE M. SNIJDERS, RICHARD SEGRAVES, STEPHANIE BLACKWOOD, DANIEL PINKEL, AND DONNA G. ALBERTSON 47.Genotyping of Single Nucleotide Polymorphisms by Minisequencing Using Tag Arrays ............................ 455 LOVISA LOVMAR, SNAEVAR SIGURDSSON, AND ANN-CHRISTINE SYVANEN 48.Single Nucleotide Polymorphism Analysis by Matrix- Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry .......................................... 463 PAMELA WHITTAKER, SUZANNAH BUMPSTEAD, KATE DOWNES, JILUR GHORI, AND PANOS DELOUKAS 49.Single Nucleotide Polymorphism Analysis by ZipCode- Tagged Microspheres ........................................ 471 J. DAVID TAYLOR, J. DAVID BRILEY, DAVID P. YARNALL, AND JINGWEN CHEN 50.Polymerase Chain Reaction-Based Amplification Method of Retaining the Quantitative Difference between Two Complex Genomes ............................................ 477 GANG WANG, BRENDAN D. PRICE, AND G. MIKE MAKRIGIORGOS PART H. TRANSGENIC, KNOCKOUTS, AND KNOCKDOWN METHODS Section 17. Transgenic, Knockouts and Knockdown Methods 51.Production of Transgenic Mice by Pronuclear Microinjection ............................................. 487 JON W. GORDON 52.Gene Targeting by Homologous Recombination in Embryonic Stem Cells ................................................. 491 AHMED MANSOURI 53.Conditional Knockouts: Cre-lox Systems ..................... 501 DANIEL METZGER, MEI LI, ARUP KUMAR INDRA, MICHAEL SCHULER, AND PIERRE CHAMBON 54.RNAi-Mediated Gene Silencing in Mammalian Cells ............ 511 DEREK M. DYKXHOORN 55.Antisense Oligonucleotides ................................. 523 ERICH KOLLER AND NICHOLAS M. DEAN