Preface ......................................................... v
Contributors ................................................... ix
1 Drosophila melanogaster and the Development of Biology in
the 20th Century
Alfonso Martinez Arias ...................................... 1
2 Getting Started: An Overview on Raising and Handling
Drosophila
Hugo Stocker and Peter Gallant ............................. 27
3 FlyBase: A Database for the Drosophila Research Community
Rachel Drysdale and the FlyBase Consortium ................. 45
4 The Use of P-Element Transposons to Generate Transgenic
Flies
André Bachmann and Elisabeth Knust ......................... 67
5 The GAL4 System: A Versatile System for the Expression of
Genes
David A. Elliott and Andrea H. Brand ....................... 79
6 P-Element Mutagenesis
Thomas Hummel and Christian Klämbt ......................... 97
7 EMS Screens: From Mutagenesis to Screening and Mapping
Christian Bökel ........................................... 119
8 RNAi Screening in Cultured Drosophila Cells
Sandra Steinbrink and Michael Boutros ..................... 139
9 Methods for Homologous Recombination in Drosophila
Keith A. Maggert, Wei J. Gong, and Kent G. Golic .......... 155
10 Recombinases and Their Use in Gene Activation, Gene
Inactivation, and Transgenesis
Johannes Bischof and Konrad Basier ........................ 175
11 Cuticle Preparation of Drosophila Embryos and Larvae
Cyrille Alexandre ......................................... 197
12 Immunolabeling of Embryos
H.-Arno J. Müller ......................................... 207
13 Imaging Cellular and Molecular Dynamics in Live Embryos
Using Fluorescent Proteins
Matthieu Cavey and Thomas Lecuit .......................... 219
14 Analysis of Neuromuscular Junctions: Histology and
In Vivo Imaging
Andreas Schmid and Stephan J. Sigrist ..................... 239
15 Immunolabeling of Imaginal Discs
Thomas Klein .............................................. 253
16 Imaging Drosophila Pupal Wing Morphogenesis
Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande,
and Suzanne Eaton ......................................... 265
17 Microscopic Analysis of the Adult Drosophila Retina
Using Semithin Plastic Sections
Konstantin Gaengel and Marek Mlodzik ...................... 277
18 Fluorescent In Situ Hybridization Protocols in
Drosophila Embryos and Tissues
Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause .... 289
19 Linear RNA Amplification for the Production of
Microarray Hybridization Probes
Ansgar Klebes and Thomas B. Kornberg ...................... 303
20 An Overview of the Identification, Detection, and
Functional Analysis of Drosophila MicroRNAs
Nicholas S. Sokol ......................................... 319
21 Extraction and Immunoblotting of Proteins From Embryos
Andreas Wodarz ............................................ 335
22 Purification of Drosophila Protein Complexes for Mass
Spectrometry
Christoph Jüschke and Jürgen A. Knoblich .................. 347
23 Mass Production of Drosophila Embryos and
Chromatographic Purification of Native Protein Complexes
Natascha Kunert and Alexander Brehm ....................... 359
24 Flow Cytometric Analysis of Drosophila Cells
Aida Flor A. de la Cruz and Bruce A. Edgar ................ 373
25 Drosophila Cell Lines as Model Systems and as
an Experimental Tool
Buzz Baum and Lucy Cherbas ................................ 391
Appendix: Phenotypic Markers in Drosophila
Jennifer Childress and Georg Halder ....................... 425
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