Channarayappa. Molecular biotechnology: principles and practices. - Hyderguda, Hyderabad; London: Universities Press, 2007. - 1217 p.(Biotechnology). - ISBN 1-4200-5157-1  Место хранения:   040 | Институт биофизики СО РАН | Красноярск | Библиотека

Оглавление / Contents

Preface ........................................................ ix PART I: Introduction to Biotechnology ...................... 1-35 1. Biotechnology: Scope and Importance ................... 2 2. Biosafety and Good Laboratory Practices .............. 17 PART II: Advanced Techniques in Molecular Biology ......... 36-124 3. Techniques of Cell Fractionation and Centrifugation .. 37 4. Chemical Synthesis of Nucleic Acids .................. 58 5. DNA Chip Technology and its Potential Applications ... 74 6. Bioinformatics in Biotechnology ...................... 94 PART III: Working with Nucleic Acids ...................... 125-361 7. Isolation of Nucleic Acids .......................... 126 8. Measuring Nucleic Acid Concentration and Purity ..... 166 9. Electrophoretic Techniques .......................... 191 10. DNA Sequencing ...................................... 232 11. Genetic Maps and Marker Analysis .................... 247 12. Polymerase Chain Reaction (PCR) ..................... 288 13. In Situ Hybridization ............................... 317 PART IV: Recombinant DNA and Genetic Engineering ......... 362-547 14. Fundamentals of Recombinant DNA Technology .......... 363 15. Enzymes in Molecular Cloning ........................ 382 16. Gene Constructs and Cloning Vectors ................. 401 17. DNA Libraries ....................................... 441 18. Molecular Biology of Gene Transfer Systems .......... 473 19. Selection and Screening of Recombinant Molecules .... 511 PART V: Applications of Biotechnology ................... 548-644 20. Genetic Engineering of Microorganisms ............... 549 21. Genetic Engineering of Animals ...................... 581 22. Genetic Engineering in Plants ....................... 612 PART VI: Working with Proteins ........................... 645-785 23. Protein Purification Techniques ..................... 646 24. Protein Detection and Estimation .................... 663 25. Protein Fractionation Techniques .................... 679 26. Immunochemical Techniques ........................... 734 PART VII: Bacterial and Mammalian Cell Culture ............ 786-868 27. Biology of Bacteria ................................. 787 28. Cultivation of Mammalian Cells In vitro ............. 836 PART VIII:In Vitro Plant Cell Culture and Crop Improvement .................................... 869-1037 29. Plant Cell Culture Laboratory and Requirements ...... 870 30. Plant Culture Media, Preparation, and Culture Initiation .......................................... 887 31. Micropropagation .................................... 905 32. Cultures of Organized Tissues ....................... 925 33. Culture of Unorganized Tissues ...................... 944 34. Cryopreservation and Distribution of Clonal Material ............................................ 980 35. Measurement of Plant Cell Growth and Cytological Analysis ............................................ 992 36. Protoplast Fusion and Somaclonal Variation ......... 1003 37. Application of Plant Cell, Tissue and Organ Culture ............................................ 1026 PART IX: Environmental Biotechnology .................... 1038-1154 38. Biotechnology in Pollution Control .................. 1039 39. Biodiversity and Genetic Conservation ............... 1073 40. Bioenergy Fuel from Biomass ......................... 1107 41. Regulatory Aspects of Using Genetically-Modified Organisms ........................................... 1118 42. Intellectual Property Rights and Socio-Legal Aspects of Biotechnology ............................ 1136 Appendices ................................................... 1155 Index ........................................................ 1183