Preface ......................................................... v
Contributors ................................................... xi
1 Peptide Antibodies: Past, Present, and Future ................ 1
Gunnar Houen
2 The Structure of Natural and Recombinant Antibodies .......... 7
Hui Ma and Richard O'Kennedy
3 Prediction of Antigenic В and T Cell Epitopes Via
Energy Decomposition Analysis: Description of the
Web-Based Prediction Tool BEPPE ............................. 13
Claudio Peri, Oscar C. Solé, Dario Corrada, Alessandro
Gori, Xavier Daura, and Giorgio Colombo
4 Prediction of Antibody Epitopes ............................. 23
Morten Nielsen and Paolo Marcatili
5 Fmoc Solid-Phase Peptide Synthesis .......................... 33
Paul R. Hansen and Alberto Oddo
6 Peptide-Carrier Conjugation ................................. 51
Paul R. Hansen
7 Solid-Phase Peptide-Carrier Conjugation ..................... 59
Gunnar Houen and Dorthe T. Olsen
8 Analysis of Peptides and Conjugates by Amino Acid Analysis .. 65
Peter Højrup
9 Characterization of Synthetic Peptides by Mass
Spectrometry ................................................ 77
Bala K. Prabhala, Osman Mirza, Peter Højrup, and Paul
R. Hansen
10 Interpretation of Tandem Mass Spectrometry (MSMS) Spectra
for Peptide Analysis ........................................ 83
Karin Hjernø and Peter Højrup
11 Polyclonal Peptide Antisera ................................ 103
Tina H. Pihl, Kristin E. Illigen, and Gunnar Houen
12 Production and Screening of Monoclonal Peptide Antibodies .. 109
Nicole Hartwig Trier, Anne Mortensen, Annette Schiolborg,
and Tina Friis
13 Production of Epitope-Specific Antibodies by Immunization
with Synthetic Epitope Peptide Formulated with
CpG-DNA-Liposome Complex Without Carriers .................. 127
Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon
14 Thioredoxin-Displayed Multipeptide Immunogens .............. 137
Angelo Bolchi, Elena Canali, Andrea Santoni, Gloria
Spagnoli, Daniele Viarisio, Rosita Accardi, Massimo
Tommasino, Martin Müller, and Simone Ottonello
15 The Purification of Natural and Recombinant Pepnde
Antibodies by Affinity Chromatographie Strategies .......... 153
Hui Ma and Richard O'Kennedy
16 Isolation of Camelid Single-Domain Antibodies Against
Native Proteins Using Recombinant Multivalent Peptide
Ligands .................................................... 167
Norah A. Alturki, Kevin A. Henry, C. Roger MacKenzie, and
Mehdi Arbabi-Ghahroudi
17 Generation of TCR-Like Annbodies Using Phage Display ....... 191
Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong
V. Cheung
18 Structural Characterization of Peptide Antibodies .......... 205
Anna Chailyan and Paolo Marcatili
19 Automated High-Throughput Mapping of Linear B-Cell
Epitopes Using a Statistical Analysis of High-Density
Peptide Microarray Data .................................... 215
Thomas Østerbye and Søren Buus
20 Characterization of Peptide Antibodies by Epitope
Mapping Using Resin-Bound and Soluble Peptides ............. 229
Nicole Hartwig Trier
21 Screening and Characterization of Linear В-Cell Epitopes
by Biotinylated Peptide Libraries .......................... 241
Ida Rosenkrands and Anja Olsen
22 Bead-Based Peptide Arrays for Profiling the Specificity
of Modification State Specific Antibodies .................. 251
Angela Filomena, Yvonne Beiter, Markus F. Templin,
Thomas O. Joos, Nicole Schneiderhan-Marra, and Oliver
Poetz
23 Surface Plasmon Resonance Method to Evaluate
Anti-citrullinated Protein/Peptide Antibody Affinity to
Citrullinated Peptides ..................................... 267
Feliciana Real-Fernández, Giada Rossi, Filomena Panza,
Federico Pratesi, Paola Migliorini, and Paolo Rovero
24 Specificity Analysis of Histonc Modification Specific
Antibodies or Reading Domains on Histone Peptide Arrays .... 275
Goran Kungulovski, Ina Kycia, Rebekka Mauser, and Albert
Jeltsch
25 Prion-Specific Antibodies Produced in Wild-Type Mice ....... 285
Peter M.H. Heegaard, Ann-Louise Bergström, Heidi Gertz
Andersen, and Henriette Cordes
26 Immunoblotting with Pepnde Antibodies: Differential
Immunoreactivities Caused by Certain Amino Acid
Substitutions in a Short Peptide and Possible Effects
of Differential Refolding of the Peptide on a
Nitrocellulose or PVDF Membrane ............................ 303
Takenori Yamamoto, Taisuke Matsuo, Atsushi Yamamoto,
Ryohei Yamagoshi, Kazuto Ohkura, Masatoshi Kataoka, and
Yasuo Shinohara
27 Immunocytochemical and Immunohistochemical Staining
with Peptide Antibodies .................................... 311
Tina Friis, Klaus Boberg Pedersen, David Hougaard, and
Gunnar Hauen
28 Designing B-Cell Epitopes for Immunotherapy and Subunit
Vaccines ................................................... 327
Harinder Singh, Sudheer Gupta, Ankur Gautam, and Gajendra
P.S. Raghava
29 Enterovirus-Specific Anti-peptide Antibodies ............... 341
Chit Laa Poh, Katherine Kirk, Hui Na Chua, and Lara Grollo
30 Therapeutic HIV Peptide Vaccine ............................ 351
Anders Fomsgaard
Index ......................................................... 359
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