Preface ......................................................... v
Contributors ................................................... xi
1 Immunohistochemistry Relative to Gravity: A Simple Method
to Retain Information About Gravity for Immunolocalization
and Histochemistry ........................................... 1
Benjamin R. Harrison and Patrick H. Masson
2 A Flat Embedding Method to Orient Thin Biological Samples
for Sectioning .............................................. 13
Utku Avci and Jin Nakashima
3 Quantification of Root Gravitropic Response Using
a Constant Stimulus Feedback System ......................... 23
Chris Wolverton
4 Analysis of Gravitropic Setpoint Angle Control in
Arabidopsis ................................................. 31
Suruchi Roychoudhry and Stefan Kepinski
5 Imaging of Dynamic Ion Signaling During Root Gravitropism ... 43
Gabriele В. Monshausen
6 Live Cell Imaging of Cytoskeletal and Organelle Dynamics
in Gravity-Sensing Cells in Plant Gravitropism .............. 57
Moritaka Nakamura, Masatsugu Toyota, Masao Tasaka, and
Miyo Terao Morita
7 Auxin Carrier and Signaling Dynamics During Gravitropic
Root Growth ................................................. 71
Mugurel I. Feraru, Jürgen Kleine-Vehn, and Elena Feraru
8 Imaging and Quantitative Methods for Studying Cytoskeletal
Rearrangements During Root Development and Gravitropism ..... 81
Eveline Jacques, Darren M. Wells, Malcolm J. Bennett, and
Kris Vissenberg
9 Methods for RNA Profiling of Gravi-Responding Plant
Tissues ..................................................... 91
Jyoti Dalal, Eric Land, Naresb Vasani, Luyan He, Caroline
Smith, Maria Rodriguez-Welsh, Imara Y. Perera, and Heike
Sederoff
10 Proteomic Approaches and Their Application to Plant
Gravitropism ............................................... 119
Proma Basu, Darron R. Luesse, and Sarah E. Wyatt
11 Assays for Root Hydrotropism and Response to Water Stress .. 133
Delfeena Eapen, Jesús J. Martinez, and Gladys I. Cassab
12 Evaluating Mechano-Transduction and Touch Responses in
Plant Roots ................................................ 143
Sarah J. Swanson, Richard Barker, Yonggeng Yе, and Simon
Gilroy
13 Expressing and Characterizing Mechanosensitive Channels
in Xenopus Oocytes ......................................... 151
Grigory Maksaev and Elizabeth S. Haswell
14 Flowering Shoots of Ornamental Crops as a Model to Study
Cellular and Molecular Aspects of Plant Gravitropism ....... 171
Sonia Philosoph-Hadas, Haya Friedman, and Shimon Meir
15 Studying Molecular Changes During Gravity Perception
and Response in a Single Cell .............................. 199
Ashley E. Cannon, Mari L. Salmi, Thomas J. Bushart, and
Stanley J. Roux
16 Live Cell and Immuno-Labeling Techniques to Study
Gravitational Effects on Single Plant Cells ................ 209
Youssef Chebli and Anja Geitmann
17 Use of High Gradient Magnetic Fields to Evaluate Gravity
Perception and Response Mechanisms in Plants and Algae ..... 227
Karl H. Hasenstein
18 Use of Microgravity Simulators for Plant Biological
Studies .................................................... 239
Raul Herranz, Miguel A. Valbuena, Aránzazu Manzano,
Khaled Y. Kamal, and F. Javier Medina
19 Conducting Plant Experiments in Space ...................... 255
John Z. Kiss
20 Spaceflight Exploration in Plant Gravitational Biology ..... 285
Anna-Lisa Paul and Robert J. Ferl
21 Hypergravity Experiments to Evaluate Gravity Resistance
Mechanisms in Plants ....................................... 307
Kouichi Saga, Sachiko Yano, Shouhei Matsumoto, and
Takayuki Hoson
Index ......................................................... 321
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