Preface ......................................................... v
Contributors ................................................... ix
1 Establishment of New Lines of Human Embryonic Stem Cells:
Evolution of the Methodology ................................. 1
Ana Maria Fraga, Érica Sara Souza de Araújo, Raquel
Stabellini, Naja Vergani, and Lygia V. Pereira
2 Human Embryonic Stem Cells Derived in Xeno-Free Conditions .. 13
Amparo Galán and Carlos Simón
3 Procedures for Derivation and Characterisation of Human
Embryonic Stem Cells from Odense, Denmark ................... 33
Linda Harkness and Moustapha Kassem
4 Principles for Derivation of Human Embryonic Stem Cells ..... 53
Mikael C.O. Englund, Catharina Ellerström, Katarina
Andersson, Karin Noaksson, and Johan Hyllner
5 Derivation and Maintenance of Undifferentiated Human
Embryonic Stem Cells ........................................ 69
Guoliang Meng and Derrick E. Rancourt
6 Establishment of hESC Lines from the Inner Cell Mass of
Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell
Stage Embryos ............................................... 81
Ileana Mateizel, Mieke Geens, Hilde Van de Velde, and
Karen Sermon
7 Analysis of LINE-1 Expression in Human Pluripotent Cells ... 113
Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia,
Santiago Morell, and Jose L. Garcia-Perez
8 Characterization and Gene Expression Profiling of Five
Human Embryonic Stem Cell Lines Derived in Taiwan .......... 127
Steven Shoei-Lung Li
9 Derivation of Human Embryonic Stem Cell Lines from Poor
Quality Embryos ............................................ 151
Ronak Shetty and Maneesba S. Inamdar
10 Derivation, Expansion, and Characterization of Human
Embryonic Stem Cell Lines from Aneuploid Embryos ........... 163
Juan-Carlos Biancotti and Neta Lavon
11 Mutated Human Embryonic Stem Cells for the Study of Human
Genetic Disorders .......................................... 179
Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, and Dalit
Ben-Tosef
12 Single-Cell Enzymatic Dissociation of hESC Lines
OxFl-OxF4 and Culture in Feeder-Free Conditions ............ 209
Frances A. Brook
13 Protocol for Expansion of Undifferentiated Human
Embryonic and Pluripotent Stem Cells in Suspension ......... 217
Hossein Baharvand, Mebran Rezaei Larijani, and Maryam
Yousefi
14 Suspension Bioreactor Expansion of Undifferentiated Human
Embryonic Stem Cells ....................................... 227
Roman Krawetz and Derrick E. Rancourt
15 Derivation, Propagation, and Characterization of
Neuroprogenitors from Pluripotent Stem Cells (hESCs and
hiPSCs) .................................................... 237
Khun-Hong Lie, Henry C.Y. Chung, and Kuldip S. Sidhu
16 Comparison of Neural Differentiation Potential of Human
Pluripotent Stem Cell Lines Using a Quantitative Neural
Differentiation Protocol ................................... 247
Dezhong Yin, Tahereh Tavakoli, Wei-Qiang Gao, and Wu Ma
17 Array-Comparative Genomic Hybridization Characterization
of Human Pluripotent Stem Cells ............................ 261
Aaron M. Elliott, Kristi A. Hohenstein Elliott, and Anja
Kammesheidt
18 Chromatin Immunoprecipitation-Based Analysis of Gene
Regulatory Networks Operative in Human Embryonic Stem
Cells ...................................................... 269
Marc Jung and James Adjaye
19 Analysis of the Methylome of Human Embryonic Stem Cells
Employing Methylated DNA Immunoprecipitation Coupled
to Next-Generation Sequencing .............................. 281
Christina Grimm and James Adjaye
20 Stable Isotope Labelling with Amino Acids in Cell Culture
for Human Embryonic Stem Cell Proteomic Analysis ........... 297
Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem,
and Blagoy Blagoev
Index ......................................................... 307
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