Petrasek Z. Fluorescence fluctuation methods in biophysical sciences: habilitationsschrift (Dresden, 2006). - ОГЛАВЛЕНИЕ / CONTENTS

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ОбложкаPetrasek Z. Fluorescence fluctuation methods in biophysical sciences: habilitationsschrift. - Dresden: [Technische Universitat Dresden], 2006. - 1 vol. (pag. var.): ill. - Bibliogr. at the end of the art.
 

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Оглавление / Contents
 
1  Summary ...................................................... 3
2  Fluctuation methods .......................................... 5
   2.1  Introduction ............................................ 5
   2.2  Fluorescence fluctuation methods, FCS ................... 6
        2.2.1  Principle and experimental realization ........... 6
        2.2.2  Theory ........................................... 8
        2.2.3  Extensions of FCS and combination with other
               techniques ...................................... 11
        2.2.4  Practical aspects ............................... 11
3  Spatio-temporal correlations ................................ 13
   3.1  Fixed configuration .................................... 13
   3.2  Scanning FCS ........................................... 14
4  Two-photon excitation ....................................... 19
   4.1  Principle .............................................. 19
   4.2  Two-photon excitation in fluorescence microscopy ....... 19
        4.2.1  Two-photon excitation in FCS .................... 20
        4.2.2  Saturation ...................................... 20
   4.3  Photobleaching ......................................... 22
        4.3.1  Photobleaching mechanisms ....................... 24
        4.3.2  Reduction of photobleaching ..................... 25
5  Biophysical applications of fluctuation methods ............. 27
6  Perspectives ................................................ 29
7  References .................................................. 32
8  Included articles ........................................... 41
A1 Fluctuations as a source of information in fluorescence
   microscopy .................................................. 41
A2 Scanning fluorescence correlation spectroscopy .............. 55
A3 Simultaneous two-photon fluorescence correlation
   spectroscopy and lifetime imaging of dye molecules in
   submicrometer fluidic structures ............................ 81
A4 Precise measurement of diffusion coefficients using
   scanning fluorescence correlation spectroscopy .............. 91
A5 Circular scanning fluorescence correlation spectroscopy on
    membranes ................................................. 105


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