Preface ......................................................... v
Contributors ................................................... ix
Part I: Tissue Processing
1 Tissue Preparation for Histochemistry: Fixation,
Embedding, and Antigen Retrieval for Light Microscopy ........ 3
Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker
2 An Optimized RNA Extraction Method from Archival Formalin-
Fixed Paraffin-Embedded Tissue .............................. 19
Joon-Yong Chung and Stephen M. Hewitt
3 Laser-Capture Microdissection and Pressure Catapulting for
the Analysis of Gene Expression in the Renal Glomerulus ..... 29
Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly
Part II: Staining Techniques
4 Immunofluorescence Detection of the Cytoskeleton and
Extracellular Matrix in Tissue and Cultured Cells ........... 43
Josiane Smith-Clerc and Boris Hinz
5 Double Immunohistochemistry with Horseradish Peroxidase
and Alkaline Phosphatase Detection Systems .................. 59
Vincent Sarrazy and Alexis Desmoulière
6 Retrogradely Transported Neuronal Tracers Combined with
Immunohistochemistry Using Free-Floating Brain Sections ..... 73
Emilio Badoer
7 High-Pressure Freezing, Chemical Fixation and Freeze-
Substitution for Immuno-electron Microscopy ................. 87
Christian Mühlfeld
8 Lectin Histochemistry for Light and Electron Microscopy .... 103
Su Ее Wong, Catherine E. Winbanks, Chrishan S. Samuel,
and Tim D. Hewitson
9 Duplex In Situ Hybridization in the Study of Gene
Co-regulation in the Vertebrate Brain ...................... 115
Raphael Pinaud and Jin K. Jeong
10 Special Stains for Extracellular Matrix .................... 131
Andrea Monte-Alto-Costa and Luís Cristóvão Porto
11 Active Staining of Mouse Embryos for Magnetic Resonance
Microscopy ................................................. 141
Alexandra Petiet and G. Allan Johnson
12 Immunohistochemical Detection of Tumour Hypoxia ............ 151
Richard J. Young and Andreas Möller
13 In Situ Localization of Apoptosis Using TUNEL .............. 161
Tim D. Hewitson and Ian A. Darby
Part III: Imaging Techniques
14 Use of Confocal Microscopy for Three-Dimensional Imaging
of Neurons in the Spinal Cord .............................. 173
Martin Stebbing, Simon Potocnik, Pinglu Yе, and Emilio
Badoer
15 High-Resolution Confocal Imaging in Tissue ................. 183
Verena C. Wimmer and Andreas Möller
16 Software-Based Stacking Techniques to Enhance Depth of
Field and Dynamic Range in Digital Photomicrography ........ 193
Jörg Piper
17 Image Analysis and Quantitative Morphology ................. 211
Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-
Santos, and Marcia Barbosa Aguila
Subject Index ................................................. 227
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